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Article

Eriocalyxin B blocks human SW1116 colon cancer cell proliferation, migration, invasion, cell cycle progression and angiogenesis via the JAK2/STAT3 signaling pathway

  • Authors:
    • Yun‑Min Lu
    • Wei Chen
    • Jin‑Shui Zhu
    • Wei‑Xiong Chen
    • Ni‑Wei Chen
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China
  • Pages: 2235-2240
    |
    Published online on: January 19, 2016
       https://doi.org/10.3892/mmr.2016.4800
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Abstract

Eriocalyxin B, a natural ent-kaurene diterpene compound, has been shown to prevent carcinogenesis and tumor development. However, little is known regarding the mechanism underlying the antitumor activity of Eriocalyxin B in human colon cancer. The aim of the present study was to examine the role of Eriocalyxin B in SW1116 cells, and to verify the hypothesis that the Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) signaling pathway may serve as a therapeutic target in human colon cancer treatment. Cell proliferation was measured with a Cell Counting kit‑8 assay, and the cell cycle was assessed by flow cytometry. Cell migration and invasion were measured by Transwell analysis. In addition, western blot analysis was performed to detect the protein expression levels in SW1116 cells treated with various concentrations of Eriocalyxin B. The results demonstrated that 1 µmol/l Eriocalyxin B was effective at inhibiting JAK2 and STAT3 phosphorylation, followed by the downregulation of JAK2 and STAT3 downstream target expression, which resulted in the inhibition of cell proliferation, migration, invasion and angiogenesis. Eriocalyxin B also suppressed the expression of proliferation‑associated protein (proliferating cell nuclear antigen) and angiogenesis‑associated proteins (vascular endothelial growth factor and vascular endothelial growth factor receptor 2), as well as that of migration- and invasion‑associated proteins (matrix metalloproteinase 2 and 9). These results suggested that Eriocalyxin B may suppress JAK2/STAT3 signaling, and thus act as a therapeutic or preventive agent in the treatment of human colon cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Lu YM, Chen W, Zhu JS, Chen WX and Chen NW: Eriocalyxin B blocks human SW1116 colon cancer cell proliferation, migration, invasion, cell cycle progression and angiogenesis via the JAK2/STAT3 signaling pathway. Mol Med Rep 13: 2235-2240, 2016.
APA
Lu, Y., Chen, W., Zhu, J., Chen, W., & Chen, N. (2016). Eriocalyxin B blocks human SW1116 colon cancer cell proliferation, migration, invasion, cell cycle progression and angiogenesis via the JAK2/STAT3 signaling pathway. Molecular Medicine Reports, 13, 2235-2240. https://doi.org/10.3892/mmr.2016.4800
MLA
Lu, Y., Chen, W., Zhu, J., Chen, W., Chen, N."Eriocalyxin B blocks human SW1116 colon cancer cell proliferation, migration, invasion, cell cycle progression and angiogenesis via the JAK2/STAT3 signaling pathway". Molecular Medicine Reports 13.3 (2016): 2235-2240.
Chicago
Lu, Y., Chen, W., Zhu, J., Chen, W., Chen, N."Eriocalyxin B blocks human SW1116 colon cancer cell proliferation, migration, invasion, cell cycle progression and angiogenesis via the JAK2/STAT3 signaling pathway". Molecular Medicine Reports 13, no. 3 (2016): 2235-2240. https://doi.org/10.3892/mmr.2016.4800
Copy and paste a formatted citation
x
Spandidos Publications style
Lu YM, Chen W, Zhu JS, Chen WX and Chen NW: Eriocalyxin B blocks human SW1116 colon cancer cell proliferation, migration, invasion, cell cycle progression and angiogenesis via the JAK2/STAT3 signaling pathway. Mol Med Rep 13: 2235-2240, 2016.
APA
Lu, Y., Chen, W., Zhu, J., Chen, W., & Chen, N. (2016). Eriocalyxin B blocks human SW1116 colon cancer cell proliferation, migration, invasion, cell cycle progression and angiogenesis via the JAK2/STAT3 signaling pathway. Molecular Medicine Reports, 13, 2235-2240. https://doi.org/10.3892/mmr.2016.4800
MLA
Lu, Y., Chen, W., Zhu, J., Chen, W., Chen, N."Eriocalyxin B blocks human SW1116 colon cancer cell proliferation, migration, invasion, cell cycle progression and angiogenesis via the JAK2/STAT3 signaling pathway". Molecular Medicine Reports 13.3 (2016): 2235-2240.
Chicago
Lu, Y., Chen, W., Zhu, J., Chen, W., Chen, N."Eriocalyxin B blocks human SW1116 colon cancer cell proliferation, migration, invasion, cell cycle progression and angiogenesis via the JAK2/STAT3 signaling pathway". Molecular Medicine Reports 13, no. 3 (2016): 2235-2240. https://doi.org/10.3892/mmr.2016.4800
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