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Article

Monoacylglycerol lipase inhibitor JZL184 regulates apoptosis and migration of colorectal cancer cells

  • Authors:
    • Muyuan Ma
    • Jie Bai
    • Ye Ling
    • Weilong Chang
    • Gengchen Xie
    • Ruidong Li
    • Guobin Wang
    • Kaixiong Tao
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Surgery II, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
  • Pages: 2850-2856
    |
    Published online on: January 29, 2016
       https://doi.org/10.3892/mmr.2016.4829
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Abstract

Monoacylglycerol lipase (MAGL) is involved in the degradation of triacylglycerol. Previous studies have demonstrated that MAGL regulates tumor growth and metastasis via fatty acid networks, and is associated with colorectal cancer. JZL184 is a MAGL inhibitor, which in the present study was administered to colorectal cancer cell lines, resulting in decreased tumor proliferation, increased apoptosis and increased tumor cell sensitivity to 5-fluorouracil. B‑cell lymphoma 2 (Bcl‑2) and Bcl‑2‑associated X protein (Bax) are key proteins in apoptosis. The expression levels of Bcl‑2/Bax were determined in colorectal cancer cell lines following JZL184 administration, and it was observed that the mRNA and protein expression levels of Bcl‑2 were decreased, whereas the expression levels of Bax were increased. These results indicated that JZL184 may induce tumor cell apoptosis by regulating the expression of Bcl‑2 and Bax. Epithelial-mesenchymal transition (EMT) is closely associated with metastasis. Administration of JZL184 in various malignant colorectal cancer cell lines suppressed migration and altered the expression of EMT markers; E‑cadherin was increased, whereas the expression levels of vimentin and zinc finger protein SNAI1 were decreased. These results suggested that JZL184 was able to regulate the EMT process, in order to control the migration of colorectal cancer cells, particularly in tumors with a stronger metastatic capability. Therefore, in colorectal cancer, MAGL may be considered a potential therapeutic target and JZL184 may be a possible therapeutic agent.
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1 

King AR, Lodola A, Carmi C, Fu J, Mor M and Piomelli D: A critical cysteine residue in monoacylglycerol lipase is targeted by a new class of isothiazolinone-based enzyme inhibitors. Br J Pharmacol. 157:974–983. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Blankman JL, Simon GM and Cravatt BF: A comprehensive profile of brain enzymes that hydrolyze the endocannabinoid 2-arachidonoylglycerol. Chem Biol. 14:1347–1356. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Pisanti S, Picardi P, D'Alessandro A, Laezza C and Bifulco M: The endocannabinoid signaling system in cancer. Trends Pharmacol Sci. 34:273–282. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Hermanson DJ and Marnett LJ: Cannabinoids, endocannabinoids, and cancer. Cancer Metastasis Rev. 30:599–612. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Panikashvili D, Simeonidou C, Ben-Shabat S, Hanus L, Breuer A, Mechoulam R and Shohami E: An endogenous cannabinoid (2-AG) is neuroprotective after brain injury. Nature. 413:527–531. 2001. View Article : Google Scholar : PubMed/NCBI

6 

Long JZ, Li W, Booker L, Burston JJ, Kinsey SG, Schlosburg JE, Pavón FJ, Serrano AM, Selley DE, Parsons LH, et al: Selective blockade of 2-arachidonoylglycerol hydrolysis produces cannabinoid behavioral effects. Nat Chem Biol. 5:37–44. 2009. View Article : Google Scholar :

7 

Nomura DK, Long JZ, Niessen S, Hoover HS, Ng SW and Cravatt BF: Monoacylglycerol lipase regulates a fatty acid network that promotes cancer pathogenesis. Cell. 140:49–61. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Nomura DK, Lombardi DP, Chang JW, Niessen S, Ward AM, Long JZ, Hoover HH and Cravatt BF: Monoacylglycerol lipase exerts dual control over endocannabinoid and fatty acid pathways to support prostate cancer. Chem Biol. 18:846–856. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Zlobec I and Lugli A: Epithelial mesenchymal transition and tumor budding in aggressive colorectal cancer: Tumor budding as oncotarget. Oncotarget. 1:651–661. 2010. View Article : Google Scholar

10 

Joyce T, Cantarella D, Isella C, Medico E and Pintzas A: A molecular signature for epithelial to mesenchymal transition in a human colon cancer cell system is revealed by large-scale microarray analysis. Clin Exp Metastasis. 26:569–587. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Ye L, Zhang B, Seviour EG, Tao KX, Liu XH, Ling Y, Chen JY and Wang GB: Monoacylglycerol lipase (MAGL) knockdown inhibits tumor cells growth in colorectal cancer. Cancer Lett. 307:6–17. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Hector S and Prehn JH: Apoptosis signaling proteins as prognostic biomarkers in colorectal cancer: A review. Biochim Biophys Acta. 1795:117–129. 2009.PubMed/NCBI

13 

Prabhudesai SG, Rekhraj S, Roberts G, Darzi AW and Ziprin P: Apoptosis and chemo-resistance in colorectal cancer. J Surg Oncol. 96:77–88. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Oltvai ZN, Milliman CL and Korsmeyer SJ: Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell. 74:609–619. 1993. View Article : Google Scholar : PubMed/NCBI

15 

O'Leary DP, Bhatt L, Woolley JF, Gough DR, Wang JH, Cotter TG and Redmond HP: TLR-4 signalling accelerates colon cancer cell adhesion via NF-κB mediated transcriptional up-regulation of Nox-1. PLoS One. 7:e441762012. View Article : Google Scholar

16 

Bai J, Chen J, Ma M, Cai M, Xu F, Wang G, Tao K and Shuai X: Inhibiting enhancer of zeste homolog 2 promotes cellular senescence in gastric cancer cells SGC-7901 by activation of p21 and p16. DNA Cell Biol. 33:337–344. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Leng Z, Tao K, Xia Q, Tan J, Yue Z, Chen J, Xi H, Li J and Zheng H: Krüppel-like factor 4 acts as an oncogene in colon cancer stem cell-enriched spheroid cells. PLoS One. 8:e560822013. View Article : Google Scholar

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar

19 

Croker BA, O'Donnell JA, Nowell CJ, Metcalf D, Dewson G, Campbell KJ, Rogers KL, Hu Y, Smyth GK, Zhang JG, et al: Fas-mediated neutrophil apoptosis is accelerated by Bid, Bak, and Bax and inhibited by Bcl-2 and Mcl-1. Proc Natl Acad Sci USA. 108:13135–13140. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Lee JG, McKinney KQ, Pavlopoulos AJ, Park JH and Hwang S: Identification of anti-metastatic drug and natural compound targets in isogenic colorectal cancer cells. J Proteomics. 113:326–336. 2015. View Article : Google Scholar

21 

Schlosburg JE, Blankman JL, Long JZ, Nomura DK, Pan B, Kinsey SG, Nguyen PT, Ramesh D, Booker L, Burston JJ, et al: Chronic monoacylglycerol lipase blockade causes functional antagonism of the endocannabinoid system. Nat Neurosci. 13:1113–1119. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Gustafsson SB, Palmqvist R, Henriksson ML, Dahlin AM, Edin S, Jacobsson SO, Öberg Å and Fowler CJ: High tumour cannabinoid CB1 receptor immunoreactivity negatively impacts disease-specific survival in stage II microsatellite stable colorectal cancer. PLoS One. 6:e230032011. View Article : Google Scholar : PubMed/NCBI

23 

Guzmán M: Cannabinoids: Potential anticancer agents. Nat Rev Cancer. 3:745–755. 2003. View Article : Google Scholar : PubMed/NCBI

24 

Zeestraten EC, Benard A, Reimers MS, Schouten PC, Liefers GJ, van de Velde CJ and Kuppen PJ: The prognostic value of the apoptosis pathway in colorectal cancer: A review of the literature on biomarkers identified by immunohistochemistry. Biomark Cancer. 5:13–29. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Moreno A, Figueras A, Lloveras B, Escobedo A, Griera E, Sierra A and Fabra A: Apoptosis in ductal carcinoma in situ of the breast. Breast J. 7:245–248. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Choi BH, Kim W, Wang QC, Kim DC, Tan SN, Yong JW, Kim KT and Yoon HS: Kinetin riboside preferentially induces apoptosis by modulating Bcl-2 family proteins and caspase-3 in cancer cells. Cancer Lett. 261:37–45. 2008. View Article : Google Scholar

27 

Zhao S, Konopleva M, Cabreira-Hansen M, Xie Z, Hu W, Milella M, Estrov Z, Mills GB and Andreeff M: Inhibition of phosphatidylinositol 3-kinase dephosphorylates BAD and promotes apoptosis in myeloid leukemias. Leukemia. 18:267–275. 2004. View Article : Google Scholar

28 

Kalluri R and Weinberg RA: The basics of epithelialmesenchymal transition. J Clin Invest. 119:1420–1428. 2009. View Article : Google Scholar : PubMed/NCBI

29 

Satelli A and Li S: Vimentin in cancer and its potential as a molecular target for cancer therapy. Cell Mol Life Sci. 68:3033–3046. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Joyce T, Cantarella D, Isella C, Medico E and Pintzas A: A molecular signature for Epithelial to Mesenchymal transition in a human colon cancer cell system is revealed by large-scale microarray analysis. Clin Exp Metastasis. 26:569–587. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Hu WR, Lian YF, Peng LX, Lei JJ, Deng CC, Xu M, Feng QS, Chen LZ, Bei JX and Zeng YX: Monoacylglycerol lipase promotes metastases in nasopharyngeal carcinoma. Int J Clin Exp Pathol. 7:3704–3713. 2014.PubMed/NCBI

32 

Neil JR, Johnson KM, Nemenoff RA and Schiemann WP: Cox-2 inactivates Smad signaling and enhances EMT stimulated by TGF-beta through a PGE2-dependent mechanism. Carcinogenesis. 29:2227–2235. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ma M, Bai J, Ling Y, Chang W, Xie G, Li R, Wang G and Tao K: Monoacylglycerol lipase inhibitor JZL184 regulates apoptosis and migration of colorectal cancer cells. Mol Med Rep 13: 2850-2856, 2016.
APA
Ma, M., Bai, J., Ling, Y., Chang, W., Xie, G., Li, R. ... Tao, K. (2016). Monoacylglycerol lipase inhibitor JZL184 regulates apoptosis and migration of colorectal cancer cells. Molecular Medicine Reports, 13, 2850-2856. https://doi.org/10.3892/mmr.2016.4829
MLA
Ma, M., Bai, J., Ling, Y., Chang, W., Xie, G., Li, R., Wang, G., Tao, K."Monoacylglycerol lipase inhibitor JZL184 regulates apoptosis and migration of colorectal cancer cells". Molecular Medicine Reports 13.3 (2016): 2850-2856.
Chicago
Ma, M., Bai, J., Ling, Y., Chang, W., Xie, G., Li, R., Wang, G., Tao, K."Monoacylglycerol lipase inhibitor JZL184 regulates apoptosis and migration of colorectal cancer cells". Molecular Medicine Reports 13, no. 3 (2016): 2850-2856. https://doi.org/10.3892/mmr.2016.4829
Copy and paste a formatted citation
x
Spandidos Publications style
Ma M, Bai J, Ling Y, Chang W, Xie G, Li R, Wang G and Tao K: Monoacylglycerol lipase inhibitor JZL184 regulates apoptosis and migration of colorectal cancer cells. Mol Med Rep 13: 2850-2856, 2016.
APA
Ma, M., Bai, J., Ling, Y., Chang, W., Xie, G., Li, R. ... Tao, K. (2016). Monoacylglycerol lipase inhibitor JZL184 regulates apoptosis and migration of colorectal cancer cells. Molecular Medicine Reports, 13, 2850-2856. https://doi.org/10.3892/mmr.2016.4829
MLA
Ma, M., Bai, J., Ling, Y., Chang, W., Xie, G., Li, R., Wang, G., Tao, K."Monoacylglycerol lipase inhibitor JZL184 regulates apoptosis and migration of colorectal cancer cells". Molecular Medicine Reports 13.3 (2016): 2850-2856.
Chicago
Ma, M., Bai, J., Ling, Y., Chang, W., Xie, G., Li, R., Wang, G., Tao, K."Monoacylglycerol lipase inhibitor JZL184 regulates apoptosis and migration of colorectal cancer cells". Molecular Medicine Reports 13, no. 3 (2016): 2850-2856. https://doi.org/10.3892/mmr.2016.4829
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