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Cardiopulmonary bypass increases pulmonary microvascular permeability through the Src kinase pathway: Involvement of caveolin-1 and vascular endothelial cadherin

  • Authors:
    • Junwen Zhang
    • Zhaolei Jiang
    • Chunrong Bao
    • Ju Mei
    • Jiaquan Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Cardiothoracic Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2918-2924
    |
    Published online on: January 29, 2016
       https://doi.org/10.3892/mmr.2016.4831
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Abstract

Changes in pulmonary microvascular permeability following cardiopulmonary bypass (CPB) and the underlying mechanisms have not yet been established. Therefore, the aim of the present study was to elucidate the alterations in pulmonary microvascular permeability following CPB and the underlying mechanism. The pulmonary microvascular permeability was measured using Evans Blue dye (EBD) exclusion, and the neutrophil infiltration and proinflammatory cytokine secretion was investigated. In addition, the activation of Src kinase and the phosphorylation of caveolin‑1 and vascular endothelial cadherin (VE‑cadherin) was examined. The results revealed that CPB increased pulmonary microvascular leakage, neutrophil count and proinflammatory cytokines in the bronchoalveolar lavage fluid, and activated Src kinase. The administration of PP2, an inhibitor of Src kinase, decreased the activation of Src kinase and attenuated the increase in pulmonary microvascular permeability observed following CPB. Two important proteins associated with vascular permeability, caveolin‑1 and VE‑cadherin, were significantly activated at 24 h in the lung tissues following CPB, which correlated with the alterations in pulmonary microvascular permeability and Src kinase. PP2 administration inhibited their activation, suggesting that they are downstream factors of Src kinase activation. The data indicated that the Src kinase pathway increased pulmonary microvascular permeability following CPB, and the activation of caveolin‑1 and VE‑cadherin may be involved. Inhibition of this pathway may provide a potential therapy for acute lung injury following cardiac surgery.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Jiang Z, Bao C, Mei J and Zhu J: Cardiopulmonary bypass increases pulmonary microvascular permeability through the Src kinase pathway: Involvement of caveolin-1 and vascular endothelial cadherin. Mol Med Rep 13: 2918-2924, 2016.
APA
Zhang, J., Jiang, Z., Bao, C., Mei, J., & Zhu, J. (2016). Cardiopulmonary bypass increases pulmonary microvascular permeability through the Src kinase pathway: Involvement of caveolin-1 and vascular endothelial cadherin. Molecular Medicine Reports, 13, 2918-2924. https://doi.org/10.3892/mmr.2016.4831
MLA
Zhang, J., Jiang, Z., Bao, C., Mei, J., Zhu, J."Cardiopulmonary bypass increases pulmonary microvascular permeability through the Src kinase pathway: Involvement of caveolin-1 and vascular endothelial cadherin". Molecular Medicine Reports 13.3 (2016): 2918-2924.
Chicago
Zhang, J., Jiang, Z., Bao, C., Mei, J., Zhu, J."Cardiopulmonary bypass increases pulmonary microvascular permeability through the Src kinase pathway: Involvement of caveolin-1 and vascular endothelial cadherin". Molecular Medicine Reports 13, no. 3 (2016): 2918-2924. https://doi.org/10.3892/mmr.2016.4831
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Jiang Z, Bao C, Mei J and Zhu J: Cardiopulmonary bypass increases pulmonary microvascular permeability through the Src kinase pathway: Involvement of caveolin-1 and vascular endothelial cadherin. Mol Med Rep 13: 2918-2924, 2016.
APA
Zhang, J., Jiang, Z., Bao, C., Mei, J., & Zhu, J. (2016). Cardiopulmonary bypass increases pulmonary microvascular permeability through the Src kinase pathway: Involvement of caveolin-1 and vascular endothelial cadherin. Molecular Medicine Reports, 13, 2918-2924. https://doi.org/10.3892/mmr.2016.4831
MLA
Zhang, J., Jiang, Z., Bao, C., Mei, J., Zhu, J."Cardiopulmonary bypass increases pulmonary microvascular permeability through the Src kinase pathway: Involvement of caveolin-1 and vascular endothelial cadherin". Molecular Medicine Reports 13.3 (2016): 2918-2924.
Chicago
Zhang, J., Jiang, Z., Bao, C., Mei, J., Zhu, J."Cardiopulmonary bypass increases pulmonary microvascular permeability through the Src kinase pathway: Involvement of caveolin-1 and vascular endothelial cadherin". Molecular Medicine Reports 13, no. 3 (2016): 2918-2924. https://doi.org/10.3892/mmr.2016.4831
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