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Inhibition of miR-29c promotes proliferation, and inhibits apoptosis and differentiation in P19 embryonic carcinoma cells

  • Authors:
    • Bin Chen
    • Guixian Song
    • Ming Liu
    • Lingmei Qian
    • Lihua Wang
    • Haitao Gu
    • Yahui Shen
  • View Affiliations / Copyright

    Affiliations: Department of Cardiothoracic Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Cardiology and Respiratory Medicine, Taizhou People's Hospital, Taizhou, Jiangsu 225300, P.R. China, Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Neonatology, Nanjing Children's Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2527-2535
    |
    Published online on: January 29, 2016
       https://doi.org/10.3892/mmr.2016.4832
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Abstract

In our previous study, the upregulation of microRNA (miR)-29c was identified in the mother of a fetus with a congenital heart defect. However, the functional and regulatory mechanisms of miR‑29c in the development of the heart remain to be elucidated. In the present study, the role and mechanism of miR‑29c inhibition in heart development were investigated in an embryonic carcinoma cell model. Inhibition of miR‑29c promoted proliferation, and suppressed the apoptosis and differentiation of P19 cells. It was also demonstrated that Wingless‑related MMTV integration site 4 (Wnt4) was a target of miR‑29c, determined using bioinformatic analysis combined with luciferase assays. The inhibition of miR‑29c stimulated the WNT4/β‑catenin pathway, promoting proliferation of the P19 cells, but suppressing their differentiation into cardiomyocytes. Furthermore, the inhibition of miR‑29c promoted the expression of B cell lymphoma‑2 and inhibited cell apoptosis. These results demonstrate the significance of miR‑29c in the process of cardiac development and suggest that miR-29c dysregulation may be associated with the occurrence of CHD. Thus, miR-29c may have therapeutic potential in the future.
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Copy and paste a formatted citation
Spandidos Publications style
Chen B, Song G, Liu M, Qian L, Wang L, Gu H and Shen Y: Inhibition of miR-29c promotes proliferation, and inhibits apoptosis and differentiation in P19 embryonic carcinoma cells. Mol Med Rep 13: 2527-2535, 2016.
APA
Chen, B., Song, G., Liu, M., Qian, L., Wang, L., Gu, H., & Shen, Y. (2016). Inhibition of miR-29c promotes proliferation, and inhibits apoptosis and differentiation in P19 embryonic carcinoma cells. Molecular Medicine Reports, 13, 2527-2535. https://doi.org/10.3892/mmr.2016.4832
MLA
Chen, B., Song, G., Liu, M., Qian, L., Wang, L., Gu, H., Shen, Y."Inhibition of miR-29c promotes proliferation, and inhibits apoptosis and differentiation in P19 embryonic carcinoma cells". Molecular Medicine Reports 13.3 (2016): 2527-2535.
Chicago
Chen, B., Song, G., Liu, M., Qian, L., Wang, L., Gu, H., Shen, Y."Inhibition of miR-29c promotes proliferation, and inhibits apoptosis and differentiation in P19 embryonic carcinoma cells". Molecular Medicine Reports 13, no. 3 (2016): 2527-2535. https://doi.org/10.3892/mmr.2016.4832
Copy and paste a formatted citation
x
Spandidos Publications style
Chen B, Song G, Liu M, Qian L, Wang L, Gu H and Shen Y: Inhibition of miR-29c promotes proliferation, and inhibits apoptosis and differentiation in P19 embryonic carcinoma cells. Mol Med Rep 13: 2527-2535, 2016.
APA
Chen, B., Song, G., Liu, M., Qian, L., Wang, L., Gu, H., & Shen, Y. (2016). Inhibition of miR-29c promotes proliferation, and inhibits apoptosis and differentiation in P19 embryonic carcinoma cells. Molecular Medicine Reports, 13, 2527-2535. https://doi.org/10.3892/mmr.2016.4832
MLA
Chen, B., Song, G., Liu, M., Qian, L., Wang, L., Gu, H., Shen, Y."Inhibition of miR-29c promotes proliferation, and inhibits apoptosis and differentiation in P19 embryonic carcinoma cells". Molecular Medicine Reports 13.3 (2016): 2527-2535.
Chicago
Chen, B., Song, G., Liu, M., Qian, L., Wang, L., Gu, H., Shen, Y."Inhibition of miR-29c promotes proliferation, and inhibits apoptosis and differentiation in P19 embryonic carcinoma cells". Molecular Medicine Reports 13, no. 3 (2016): 2527-2535. https://doi.org/10.3892/mmr.2016.4832
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