Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
March-2016 Volume 13 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2016 Volume 13 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Age-dependent changes in the protein expression levels of Redd1 and mTOR in the gerbil hippocampus during normal aging

  • Authors:
    • Hee‑Soo Choi
    • Ji Hyeon Ahn
    • Joon Ha Park
    • Moo‑Ho Won
    • Choong‑Hyun Lee
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan, South Chungcheong 31116, Republic of Korea, Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea, Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea
    Copyright: © Choi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2409-2414
    |
    Published online on: January 29, 2016
       https://doi.org/10.3892/mmr.2016.4835
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Redd1, also known as RTP801/Dig2/DDIT4, is a stress-induced protein and a negative regulator of mammalian target of rapamycin (mTOR). Redd1 is also closely associated with oxidative stress and DNA damage. In the present study, age‑related changes in the protein expression levels of mTOR and Redd1 were investigated using immunohistochemistry and western blot in the gerbil hippocampus at postnatal month (PM) 3, 6, 12 and 24. No significant differences were identified in the levels of mTOR among the experimental groups, whereas, the levels of phosphorylated mTOR decreased with age. The protein expression levels of Redd1 were observed to gradually increase with age; in the PM 24 group, the level was significantly increased (~189.2%), compared with the PM 3 group. In addition, Redd1 immunoreactivity was significantly increased in the hippocampal principal neurons of the PM 24 group, including the pyramidal cells in the hippocampus proper and granule cells in the dentate gyrus, compared with the other experimental groups. These results demonstrated that the protein expression of Redd1 in the hippocampus was markedly increased during normal aging, indicating that the age-related increase in the expression of Redd1 may be closely associated with age-related hippocampal change.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Hou XQ, Wu DW, Zhang CX, Yan R, Yang C, Rong CP, Zhang L, Chang X, Su RY, Zhang SJ, et al: Bushen-Yizhi formula ameliorates cognition deficits and attenuates oxidative stress-related neuronal apoptosis in scopolamine-induced senescence in mice. Int J Mol Med. 34:429–439. 2014.PubMed/NCBI

2 

Lister JP and Barnes CA: Neurobiological changes in the hippocampus during normative aging. Arch Neurol. 66:829–833. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Yang X, Wang JG, Ma DB, Ma XF, Zhu GJ, Zhou H, Yu CJ, Qian XY and Gao X: Myosin light chain kinase regulates hearing in mice by influencing the F-actin cytoskeleton of outer hair cells and cochleae. Int J Mol Med. 33:905–912. 2014.PubMed/NCBI

4 

Zou L, Yang R, Zhang P and Dai Y: The enhancement of amyloid precursor protein and beta-site amyloid cleavage enzyme 1 interaction: Amyloid-beta production with aging. Int J Mol Med. 25:401–407. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Cardozo-Pelaez F, Brooks PJ, Stedeford T, Song S and Sanchez-Ramos J: DNA damage, repair, and antioxidant systems in brain regions: A correlative study. Free Radic Biol Med. 28:779–785. 2000. View Article : Google Scholar : PubMed/NCBI

6 

Lee CH, Park JH, Choi JH, Yoo KY, Ryu PD and Won MH: Heat shock protein 90 and its cochaperone, p23, are markedly increased in the aged gerbil hippocampus. Exp Gerontol. 46:768–772. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Rutten BP, Korr H, Steinbusch HW and Schmitz C: The aging brain: Less neurons could be better. Mech Ageing Dev. 124:349–355. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Shigenaga MK, Hagen TM and Ames BN: Oxidative damage and mitochondrial decay in aging. Proc Natl Acad Sci USA. 91:10771–10778. 1994. View Article : Google Scholar : PubMed/NCBI

9 

Yang F, Chu X, Yin M, Liu X, Yuan H, Niu Y and Fu L: MTOR and autophagy in normal brain aging and caloric restriction ameliorating age-related cognition deficits. Behav Brain Res. 264:82–90. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Zhang R, Kadar T, Sirimanne E, MacGibbon A and Guan J: Age-related memory decline is associated with vascular and microglial degeneration in aged rats. Behav Brain Res. 235:210–217. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Jacobson L, Zhang R, Elliffe D, Chen KF, Mathai S, McCarthy D, Waldvogel H and Guan J: Correlation of cellular changes and spatial memory during aging in rats. Exp Gerontol. 43:929–938. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Rosenzweig ES and Barnes CA: Impact of aging on hippocampal function: Plasticity, network dynamics, and cognition. Prog Neurobiol. 69:143–179. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Laplante M and Sabatini DM: MTOR signaling in growth control and disease. Cell. 149:274–293. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Harrison DE, Strong R, Sharp ZD, Nelson JF, Astle CM, Flurkey K, Nadon NL, Wilkinson JE, Frenkel K, Carter CS, et al: Rapamycin fed late in life extends lifespan in genetically heterogeneous mice. Nature. 460:392–395. 2009.PubMed/NCBI

15 

Maiese K, Chong ZZ, Shang YC and Wang S: MTOR: On target for novel therapeutic strategies in the nervous system. Trends Mol Med. 19:51–60. 2013. View Article : Google Scholar :

16 

Selman C, Tullet JM, Wieser D, Irvine E, Lingard SJ, Choudhury AI, Claret M, Al-Qassab H, Carmignac D, Ramadani F, et al: Ribosomal protein S6 kinase 1 signaling regulates mammalian life span. Science. 326:140–144. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Jaworski J, Spangler S, Seeburg DP, Hoogenraad CC and Sheng M: Control of dendritic arborization by the phosphoinositide-3′-kina se-Akt-mammalian target of rapamycin pathway. J Neurosci. 25:11300–11312. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Kumar V, Zhang MX, Swank MW, Kunz J and Wu GY: Regulation of dendritic morphogenesis by Ras-PI3K-Akt-mTOR and Ras-MAPK signaling pathways. J Neurosci. 25:11288–11299. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Lee CC, Huang CC and Hsu KS: Insulin promotes dendritic spine and synapse formation by the PI3K/Akt/mTOR and Rac1 signaling pathways. Neuropharmacology. 61:867–879. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Ellisen LW: Growth control under stress: MTOR regulation through the REDD1-TSC pathway. Cell Cycle. 4:1500–1502. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Shoshani T, Faerman A, Mett I, Zelin E, Tenne T, Gorodin S, Moshel Y, Elbaz S, Budanov A, Chajut A, et al: Identification of a novel hypoxia-inducible factor 1-responsive gene, RTP801, involved in apoptosis. Mol Cell Biol. 22:2283–2293. 2002. View Article : Google Scholar : PubMed/NCBI

22 

Ellisen LW, Ramsayer KD, Johannessen CM, Yang A, Beppu H, Minda K, Oliner JD, McKeon F and Haber DA: REDD1, a developmentally regulated transcriptional target of p63 and p53, links p63 to regulation of reactive oxygen species. Mol Cell. 10:995–1005. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Lee CH, Park JH, Cho JH, Ahn JH, Yan BC, Lee JC, Shin MC, Cheon SH, Cho YS, Cho JH, et al: Changes and expressions of Redd1 in neurons and glial cells in the gerbil hippocampus proper following transient global cerebral ischemia. J Neurol Sci. 344:43–50. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Malagelada C, Jin ZH and Greene LA: RTP801 is induced in Parkinson's disease and mediates neuron death by inhibiting Akt phosphorylation/activation. J Neurosci. 28:14363–14371. 2008. View Article : Google Scholar

25 

Horak P, Crawford AR, Vadysirisack DD, Nash ZM, DeYoung MP, Sgroi D and Ellisen LW: Negative feedback control of HIF-1 through REDD1-regulated ROS suppresses tumorigenesis. Proc Natl Acad Sci USA. 107:4675–4680. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Malagelada C, López-Toledano MA, Willett RT, Jin ZH, Shelanski ML and Greene LA: RTP801/REDD1 regulates the timing of cortical neurogenesis and neuron migration. J Neurosci. 31:3186–3196. 2011. View Article : Google Scholar : PubMed/NCBI

27 

Mansfield KD, Guzy RD, Pan Y, Young RM, Cash TP, Schumacker PT and Simon MC: Mitochondrial dysfunction resulting from loss of cytochrome c impairs cellular oxygen sensing and hypoxic HIF-alpha activation. Cell Metab. 1:393–399. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Yang L, Zhang J, Zheng K, Shen H and Chen X: Long-term ginsenoside Rg1 supplementation improves age-related cognitive decline by promoting synaptic plasticity associated protein expression in C57BL/6J mice. J Gerontol A Biol Sci Med Sci. 69:282–294. 2014. View Article : Google Scholar

29 

Cheal ML: The gerbil: A unique model for research on aging. Exp Aging Res. 12:3–21. 1986. View Article : Google Scholar : PubMed/NCBI

30 

Institute of Laboratory Animal Research, Committee for the Update of the Guide for the Care and Use of Laboratory Animals and National Research Council: Guide For The Care And Use Of Laboratory Animals. 8th edition. National Academies Press; Washington, DC: pp. 2202011

31 

Lee CH and Won MH: Increased dynamin-1 and -2 protein expression in the aged gerbil hippocampus. Cell Mol Neurobiol. 34:791–796. 2014. View Article : Google Scholar : PubMed/NCBI

32 

Lazic SE: Statistical evaluation of methods for quantifying gene expression by autoradiography in histological sections. BMC Neuroscience. 10:1–15. 2009. View Article : Google Scholar

33 

Varghese F, Bukhari AB, Malhotra R and De A: IHC Profiler: an open source plugin for the quantitative evaluation and automated scoring of immunohistochemistry images of human tissue samples. PloS one. 9:e968012014. View Article : Google Scholar : PubMed/NCBI

34 

Murakami M, Ichisaka T, Maeda M, Oshiro N, Hara K, Edenhofer F, Kiyama H, Yonezawa K and Yamanaka S: MTOR is essential for growth and proliferation in early mouse embryos and embryonic stem cells. Mol Cell Biol. 24:6710–6718. 2004. View Article : Google Scholar : PubMed/NCBI

35 

Halloran J, Hussong SA, Burbank R, Podlutskaya N, Fischer KE, Sloane LB, Austad SN, Strong R, Richardson A, Hart MJ and Galvan V: Chronic inhibition of mammalian target of rapamycin by rapamycin modulates cognitive and non-cognitive components of behavior throughout lifespan in mice. Neuroscience. 223:102–113. 2012. View Article : Google Scholar : PubMed/NCBI

36 

Majumder S, Caccamo A, Medina DX, Benavides AD, Javors MA, Kraig E, Strong R, Richardson A and Oddo S: Lifelong rapamycin administration ameliorates age-dependent cognitive deficits by reducing IL-1β and enhancing NMDA signaling. Aging Cell. 11:326–335. 2012. View Article : Google Scholar : PubMed/NCBI

37 

Kolosova NG, Vitovtov AO, Muraleva NA, Akulov AE, Stefanova NA and Blagosklonny MV: Rapamycin suppresses brain aging in senescence-accelerated OXYS rats. Aging (Albany NY). 5:474–484. 2013.

38 

Jinno S and Kosaka T: Stereological estimation of numerical densities of glutamatergic principal neurons in the mouse hippocampus. Hippocampus. 20:829–840. 2010.

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Choi HS, Ahn JH, Park JH, Won MH and Lee CH: Age-dependent changes in the protein expression levels of Redd1 and mTOR in the gerbil hippocampus during normal aging. Mol Med Rep 13: 2409-2414, 2016.
APA
Choi, H., Ahn, J.H., Park, J.H., Won, M., & Lee, C. (2016). Age-dependent changes in the protein expression levels of Redd1 and mTOR in the gerbil hippocampus during normal aging. Molecular Medicine Reports, 13, 2409-2414. https://doi.org/10.3892/mmr.2016.4835
MLA
Choi, H., Ahn, J. H., Park, J. H., Won, M., Lee, C."Age-dependent changes in the protein expression levels of Redd1 and mTOR in the gerbil hippocampus during normal aging". Molecular Medicine Reports 13.3 (2016): 2409-2414.
Chicago
Choi, H., Ahn, J. H., Park, J. H., Won, M., Lee, C."Age-dependent changes in the protein expression levels of Redd1 and mTOR in the gerbil hippocampus during normal aging". Molecular Medicine Reports 13, no. 3 (2016): 2409-2414. https://doi.org/10.3892/mmr.2016.4835
Copy and paste a formatted citation
x
Spandidos Publications style
Choi HS, Ahn JH, Park JH, Won MH and Lee CH: Age-dependent changes in the protein expression levels of Redd1 and mTOR in the gerbil hippocampus during normal aging. Mol Med Rep 13: 2409-2414, 2016.
APA
Choi, H., Ahn, J.H., Park, J.H., Won, M., & Lee, C. (2016). Age-dependent changes in the protein expression levels of Redd1 and mTOR in the gerbil hippocampus during normal aging. Molecular Medicine Reports, 13, 2409-2414. https://doi.org/10.3892/mmr.2016.4835
MLA
Choi, H., Ahn, J. H., Park, J. H., Won, M., Lee, C."Age-dependent changes in the protein expression levels of Redd1 and mTOR in the gerbil hippocampus during normal aging". Molecular Medicine Reports 13.3 (2016): 2409-2414.
Chicago
Choi, H., Ahn, J. H., Park, J. H., Won, M., Lee, C."Age-dependent changes in the protein expression levels of Redd1 and mTOR in the gerbil hippocampus during normal aging". Molecular Medicine Reports 13, no. 3 (2016): 2409-2414. https://doi.org/10.3892/mmr.2016.4835
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team