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Article Open Access

Prognostic value of legumain in uveal melanoma

  • Authors:
    • Tong Wu
    • Lei Sun
    • Ying Wu
    • Rong Xiang
    • Yuwei Li
    • Weining Rong
    • Fengyuan Sun
    • Ningli Wang
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Tianjin First Central Hospital, Tianjin Medical University, Tianjin 300070, P.R. China, Department of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China, Department of Immunology, Nankai University, Tianjin 300071, P.R. China, Clinical Medical College, Tianjin Medical University, Tianjin 300070, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2377-2384
    |
    Published online on: January 29, 2016
       https://doi.org/10.3892/mmr.2016.4838
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Abstract

The present study aimed to assess the expression of legumain in uveal melanoma (UM) cell lines and primary UM specimens, and to determine the possible association between legumain expression and clinical as well as pathological characteristics to reveal its impact on the prognosis of patients with UM. Records of primary UM cases treated at Beijing Tongren Hospital and Tianjin Eye Hospital between 1996 and 2005 were retrieved for analysis and a total of 82 patients with uveal melanoma were included in the study. The expression of legumain in the formalin‑fixed and paraffin‑embedded surgical specimens of these 82 patients was determined using immunohistochemical analysis. In addition, the expression of legumain was examined in two uveal melanoma cell lines using polymerase chain reaction and western blot analyses. The association of legumain expression with clinical/pathological characteristics was analyzed using the χ2 and Fisher's exact test. In addition, the impact of legumain on the prognosis of patients with uveal melanoma was examined. Upregulation of legumain was more predominant in the highly invasive uveal melanoma cell line MUM‑2B compared with that in the MUM‑2C with low invasiveness. Of 82 primary uveal melanoma tissues, 35 exhibited high expression of legumain, while the other 47 specimens exhibited low or negative expression of legumain. High legumain expression was primarily associated with local invasion of UM. Overall survival analysis revealed that the patients with high legumain expression exhibited poorer survival than patients with low/negative legumain expression. These findings suggested that upregulation of legumain is associated with malignant behavior of UM and that legumain may be used as an negative prognostic factor as well as a therapeutic target.
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1 

Mudhar HS, Parsons MA, Sisley K, Rundle P, Singh A and Rennie IG: A critical appraisal of the prognostic and predictive factors for uveal malignant melanoma. Histopathology. 45:1–12. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Singh AD and Borden EC: Metastatic uveal melanoma. Ophthalmol Clin North Am. 18:143–150. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Desjardins L, Levy-Gabriel C, Lumbroso-Lerouic L, Sastre X, Dendale R, Couturier J, Piperno-Neumann S, Dorval T, Mariani P, Salmon R, et al: Prognostic factors for malignant uveal melanoma. Retrospective study on 2,241 patients and recent contribution of monosomy-3 research. J Fr Ophtalmol. 29:741–749. 2006.In French. View Article : Google Scholar : PubMed/NCBI

4 

Kembhavi AA, Buttle DJ, Knight CG and Barrett AJ: The two cysteine endopeptidases of legume seeds: Purification and characterization by use of specific fluorometric assays. Arch Biochem Biophys. 303:208–213. 1993. View Article : Google Scholar : PubMed/NCBI

5 

Dalton JP, Hola-Jamriska L and Brindley PJ: Asparaginyl endopeptidase activity in adult Schistosoma mansoni. Parasitology. 111:575–580. 1995. View Article : Google Scholar : PubMed/NCBI

6 

Chen JM, Dando PM, Rawlings ND, Brown MA, Young NE, Stevens RA, Hewitt E, Watts C and Barrett AJ: Cloning, isolation and characterization of mammalian legumain, an asparaginyl endopeptidase. J Biol Chem. 272:8090–8098. 1997. View Article : Google Scholar : PubMed/NCBI

7 

Liu C, Sun C, Huang H, Janda K and Edgington T: Overexpression of legumain in tumors is significant for invasion/metastasis and a candidate enzymatic target for prodrug therapy. Cancer Res. 63:2957–2964. 2003.PubMed/NCBI

8 

Murthy RV, Arbman G, Gao J, Roodman GD and Sun XF: Legumain expression in relation to clinicopathologic and biological variables in colorectal cancer. Clin Cancer Res. 11:2293–2299. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Gawenda J, Traub F, Lück HJ, Kreipe H and von Wasielewski R: Legumain expression as a prognostic factor in breast cancer patients. Breast Cancer Res Treat. 102:1–6. 2007. View Article : Google Scholar

10 

Barrett AJ and Rawlings ND: Evolutionary lines of cysteine peptidases. Biol Chem. 382:727–733. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Chen JM, Fortunato M, Stevens RA and Barrett AJ: Activation of progelatinase A by mammalian legumain, a recently discovered cysteine proteinase. Biol Chem. 382:777–783. 2001. View Article : Google Scholar : PubMed/NCBI

12 

Luo Y, Zhou H, Krueger J, Kaplan C, Lee SH, Dolman C, Markowitz D, Wu W, Liu C, Reisfeld RA and Xiang R: Targeting tumor-associated macrophages as a novel strategy against breast cancer. J Clin Invest. 116:2132–2141. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Lewēn S, Zhou H, Hu HD, Cheng T, Markowitz D, Reisfeld RA, Xiang R and Luo Y: A legumain-based minigene vaccine targets the tumor stroma and suppresses breast cancer growth and angiogenesis. Cancer Immunol Immunother. 57:507–515. 2008. View Article : Google Scholar

14 

Xiang R, Luo Y, Niethammer AG and Reisfeld RA: Oral DNA vaccines target the tumor vasculature and microenvironment and suppress tumor growth and metastasis. Immunol Rev. 222:117–128. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Bajjuri KM, Liu Y, Liu C and Sinha SC: The legumain protease-activated auristatin prodrugs suppress tumor growth and metastasis without toxicity. Chem Med Chem. 6:54–59. 2011. View Article : Google Scholar

16 

Wu W, Luo Y, Sun C, et al: Targeting cell-impermeable prodrug activation to tumor microenvironment eradicates multiple drug-resistant neoplasms. Cancer Res. 66:970–980. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Stern L, Perry R, Ofek P, Many A, Shabat D and Satchi-Fainaro R: A novel antitumor prodrug platform designed to be cleaved by the endoprotease legumain. Bioconjug Chem. 20:500–510. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Seftor EA, Meltzer PS, Kirschmann DA, Pe'er J, Maniotis AJ, Trent JM, Folberg R and Hendrix MJ: Molecular determinants of human uveal melanoma invasion and metastasis. Clin Exp Metastasis. 19:233–246. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar

20 

Briggs JJ, Haugen MH, Johansen HT, Riker AI, Abrahamson M, Fodstad Ø, Maelandsmo GM and Solberg R: Cystatin E/M suppresses legumain activity and invasion of human melanoma. BMC Cancer. 10:172010. View Article : Google Scholar : PubMed/NCBI

21 

Andrade V, Guerra M, Jardim C, Melo F, Silva W, Ortega JM, Robert M, Nathanson MH and Leite F: Nucleoplasmic calcium regulates cell proliferation through legumain. J Hepatol. 55:626–635. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Bingle L, Brown NJ and Lewis CE: The role of tumour-associated macrophages in tumour progression: Implications for new anticancer therapies. J Pathol. 196:254–265. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Clerin V, Shih HH, Deng N, Hebert G, Resmini C, Shields KM, Feldman JL, Winkler A, Albert L, Maganti V, et al: Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis. Atherosclerosis. 201:53–66. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wu T, Sun L, Wu Y, Xiang R, Li Y, Rong W, Sun F and Wang N: Prognostic value of legumain in uveal melanoma. Mol Med Rep 13: 2377-2384, 2016.
APA
Wu, T., Sun, L., Wu, Y., Xiang, R., Li, Y., Rong, W. ... Wang, N. (2016). Prognostic value of legumain in uveal melanoma. Molecular Medicine Reports, 13, 2377-2384. https://doi.org/10.3892/mmr.2016.4838
MLA
Wu, T., Sun, L., Wu, Y., Xiang, R., Li, Y., Rong, W., Sun, F., Wang, N."Prognostic value of legumain in uveal melanoma". Molecular Medicine Reports 13.3 (2016): 2377-2384.
Chicago
Wu, T., Sun, L., Wu, Y., Xiang, R., Li, Y., Rong, W., Sun, F., Wang, N."Prognostic value of legumain in uveal melanoma". Molecular Medicine Reports 13, no. 3 (2016): 2377-2384. https://doi.org/10.3892/mmr.2016.4838
Copy and paste a formatted citation
x
Spandidos Publications style
Wu T, Sun L, Wu Y, Xiang R, Li Y, Rong W, Sun F and Wang N: Prognostic value of legumain in uveal melanoma. Mol Med Rep 13: 2377-2384, 2016.
APA
Wu, T., Sun, L., Wu, Y., Xiang, R., Li, Y., Rong, W. ... Wang, N. (2016). Prognostic value of legumain in uveal melanoma. Molecular Medicine Reports, 13, 2377-2384. https://doi.org/10.3892/mmr.2016.4838
MLA
Wu, T., Sun, L., Wu, Y., Xiang, R., Li, Y., Rong, W., Sun, F., Wang, N."Prognostic value of legumain in uveal melanoma". Molecular Medicine Reports 13.3 (2016): 2377-2384.
Chicago
Wu, T., Sun, L., Wu, Y., Xiang, R., Li, Y., Rong, W., Sun, F., Wang, N."Prognostic value of legumain in uveal melanoma". Molecular Medicine Reports 13, no. 3 (2016): 2377-2384. https://doi.org/10.3892/mmr.2016.4838
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