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Electroacupuncture stimulation at CV4 prevents ovariectomy‑induced osteoporosis in rats via Wnt‑β‑catenin signaling

  • Authors:
    • Huailing Fan
    • Feng Ji
    • Ying Lin
    • Mulan Zhang
    • Wei Qin
    • Qi Zhou
    • Qiang Wu
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Medicine, Xiamen Medical College, Xiamen, Fujian 361008, P.R. China, Academy of Acupuncture and Moxibustion, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China
    Copyright: © Fan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2485-2491
    |
    Published online on: February 3, 2016
       https://doi.org/10.3892/mmr.2016.4849
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Abstract

The present study aimed to investigate the effect of electroacupuncture stimulation at CV4 (also termed Guanyuan) on femoral osteocalcin also termed bone gla protein (BGP), alkaline phosphatase (ALP), bone mineral density (BMD) and biomechanics, as well as the Wnt‑β‑catenin signaling pathway in rats with postmenopausal osteoporosis. Female Sprague‑Dawley rats (4.5‑months old) were randomly divided into sham, Ovx, CV4 and mock groups (n=10/group). With the exception of those in the sham group, the rats were ovariectomized to induce postmenopausal osteoporosis. The rats in the CV4 and mock groups were given electroacupuncture at CV4 and non‑acupoint, respectively. The rats in the Ovx model and sham groups underwent identical fixing procedures, but did not undergo electroacupuncture. Following treatment, hematoxylin and eosin staining was used to observe morphological changes in the left femoral trabecular bone, and a three‑point‑bending test was used to analyze femur biomechanics and determine the BMD. In addition, an enzyme‑linked immunosorbent assay was used to measure the serum levels of ALP/BGP and reverse transcription‑quantitative polymerase chain reaction was used detect the expression levels of Wnt3a, β‑catenin and Runx2. In the present study, it was demonstrated that electroacupuncture at CV4 significantly improved the osteoporotic morphological changes that occurred in the ovariectomized rats, increased serum ALP and BGP levels, enhanced the maximum and fracture loads, increased BMD (P<0.01), and activated the Wnt‑β‑catenin signaling pathway. These findings demonstrated that electroacupuncture stimulation at CV4 affected bone formation and promoted bone metabolism in rats with postmenopausal osteoporosis, possibly by activating the Wnt‑β‑catenin signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Fan H, Ji F, Lin Y, Zhang M, Qin W, Zhou Q and Wu Q: Electroacupuncture stimulation at CV4 prevents ovariectomy‑induced osteoporosis in rats via Wnt‑β‑catenin signaling. Mol Med Rep 13: 2485-2491, 2016.
APA
Fan, H., Ji, F., Lin, Y., Zhang, M., Qin, W., Zhou, Q., & Wu, Q. (2016). Electroacupuncture stimulation at CV4 prevents ovariectomy‑induced osteoporosis in rats via Wnt‑β‑catenin signaling. Molecular Medicine Reports, 13, 2485-2491. https://doi.org/10.3892/mmr.2016.4849
MLA
Fan, H., Ji, F., Lin, Y., Zhang, M., Qin, W., Zhou, Q., Wu, Q."Electroacupuncture stimulation at CV4 prevents ovariectomy‑induced osteoporosis in rats via Wnt‑β‑catenin signaling". Molecular Medicine Reports 13.3 (2016): 2485-2491.
Chicago
Fan, H., Ji, F., Lin, Y., Zhang, M., Qin, W., Zhou, Q., Wu, Q."Electroacupuncture stimulation at CV4 prevents ovariectomy‑induced osteoporosis in rats via Wnt‑β‑catenin signaling". Molecular Medicine Reports 13, no. 3 (2016): 2485-2491. https://doi.org/10.3892/mmr.2016.4849
Copy and paste a formatted citation
x
Spandidos Publications style
Fan H, Ji F, Lin Y, Zhang M, Qin W, Zhou Q and Wu Q: Electroacupuncture stimulation at CV4 prevents ovariectomy‑induced osteoporosis in rats via Wnt‑β‑catenin signaling. Mol Med Rep 13: 2485-2491, 2016.
APA
Fan, H., Ji, F., Lin, Y., Zhang, M., Qin, W., Zhou, Q., & Wu, Q. (2016). Electroacupuncture stimulation at CV4 prevents ovariectomy‑induced osteoporosis in rats via Wnt‑β‑catenin signaling. Molecular Medicine Reports, 13, 2485-2491. https://doi.org/10.3892/mmr.2016.4849
MLA
Fan, H., Ji, F., Lin, Y., Zhang, M., Qin, W., Zhou, Q., Wu, Q."Electroacupuncture stimulation at CV4 prevents ovariectomy‑induced osteoporosis in rats via Wnt‑β‑catenin signaling". Molecular Medicine Reports 13.3 (2016): 2485-2491.
Chicago
Fan, H., Ji, F., Lin, Y., Zhang, M., Qin, W., Zhou, Q., Wu, Q."Electroacupuncture stimulation at CV4 prevents ovariectomy‑induced osteoporosis in rats via Wnt‑β‑catenin signaling". Molecular Medicine Reports 13, no. 3 (2016): 2485-2491. https://doi.org/10.3892/mmr.2016.4849
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