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Anti‑angiogenic therapy for normalization of tumor vasculature: A potential effect of Buyang Huanwu decoction on nude mice bearing human hepatocellular carcinoma xenografts with high metastatic potential

  • Authors:
    • Liang Min
    • Wei Ling
    • Rong Hua
    • Hong Qi
    • Shenxu Chen
    • Haiqiao Wang
    • Lumen Tang
    • Wenji Shangguan
  • View Affiliations / Copyright

    Affiliations: Department of Traditional Chinese Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R. China, Department of Gastrointestinal Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R. China, Department of Hepatopancreatobiliary Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R. China
    Copyright: © Min et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2518-2526
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    Published online on: February 3, 2016
       https://doi.org/10.3892/mmr.2016.4854
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Abstract

The present study aimed to investigate the effect of Buyang Huanwu decoction (BYHWD) on tumor growth, metastasis and angiogenesis in nude mice bearing human hepatocellular carcinoma (HCC) HCCLM3 xenografts. A total of 96 nude mice bearing HCCLM3 xenografts were randomly divided into four groups: BYHWD group (LB), Yi‑qi decoction group (LY), Huo‑xue decoction group (LH) and model group (LM). Each of these groups was divided into three subgroups (n=8), which were observed on days 21, 25, 38 following treatment, respectively. The tumor weights, volumes and pulmonary metastases were recorded. The expression of CD105 and the microvessel density (MVD) were assessed, and the expression levels of vascular endothelial growth factor (VEGF), hypoxia‑inducible factor 1α (HIF‑1α), and regulator of G protein signaling 5 (RGS‑5) were analyzed using immunohistochemical staining. Compared with the LM group, no significant decrease in tumor weight or volume were observed in the herbal medicine treatment groups, the number of the metastases in the lungs decreased, whereas the expression levels of RGS‑5 and HIF‑1α decreased in the LB group on day 35. However, the expression levels of VEGF increased in the LB group on days 28 and 35 post‑treatment. The results of the present study suggested that BYHWD may inhibit angiogenesis and metastasis by affecting the expression levels of VEGF, RGS‑5 and HIF‑1α, and suggested that BYHWD may contribute to the tumor microenvironment and vasculature normalization in HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Min L, Ling W, Hua R, Qi H, Chen S, Wang H, Tang L and Shangguan W: Anti‑angiogenic therapy for normalization of tumor vasculature: A potential effect of Buyang Huanwu decoction on nude mice bearing human hepatocellular carcinoma xenografts with high metastatic potential. Mol Med Rep 13: 2518-2526, 2016.
APA
Min, L., Ling, W., Hua, R., Qi, H., Chen, S., Wang, H. ... Shangguan, W. (2016). Anti‑angiogenic therapy for normalization of tumor vasculature: A potential effect of Buyang Huanwu decoction on nude mice bearing human hepatocellular carcinoma xenografts with high metastatic potential. Molecular Medicine Reports, 13, 2518-2526. https://doi.org/10.3892/mmr.2016.4854
MLA
Min, L., Ling, W., Hua, R., Qi, H., Chen, S., Wang, H., Tang, L., Shangguan, W."Anti‑angiogenic therapy for normalization of tumor vasculature: A potential effect of Buyang Huanwu decoction on nude mice bearing human hepatocellular carcinoma xenografts with high metastatic potential". Molecular Medicine Reports 13.3 (2016): 2518-2526.
Chicago
Min, L., Ling, W., Hua, R., Qi, H., Chen, S., Wang, H., Tang, L., Shangguan, W."Anti‑angiogenic therapy for normalization of tumor vasculature: A potential effect of Buyang Huanwu decoction on nude mice bearing human hepatocellular carcinoma xenografts with high metastatic potential". Molecular Medicine Reports 13, no. 3 (2016): 2518-2526. https://doi.org/10.3892/mmr.2016.4854
Copy and paste a formatted citation
x
Spandidos Publications style
Min L, Ling W, Hua R, Qi H, Chen S, Wang H, Tang L and Shangguan W: Anti‑angiogenic therapy for normalization of tumor vasculature: A potential effect of Buyang Huanwu decoction on nude mice bearing human hepatocellular carcinoma xenografts with high metastatic potential. Mol Med Rep 13: 2518-2526, 2016.
APA
Min, L., Ling, W., Hua, R., Qi, H., Chen, S., Wang, H. ... Shangguan, W. (2016). Anti‑angiogenic therapy for normalization of tumor vasculature: A potential effect of Buyang Huanwu decoction on nude mice bearing human hepatocellular carcinoma xenografts with high metastatic potential. Molecular Medicine Reports, 13, 2518-2526. https://doi.org/10.3892/mmr.2016.4854
MLA
Min, L., Ling, W., Hua, R., Qi, H., Chen, S., Wang, H., Tang, L., Shangguan, W."Anti‑angiogenic therapy for normalization of tumor vasculature: A potential effect of Buyang Huanwu decoction on nude mice bearing human hepatocellular carcinoma xenografts with high metastatic potential". Molecular Medicine Reports 13.3 (2016): 2518-2526.
Chicago
Min, L., Ling, W., Hua, R., Qi, H., Chen, S., Wang, H., Tang, L., Shangguan, W."Anti‑angiogenic therapy for normalization of tumor vasculature: A potential effect of Buyang Huanwu decoction on nude mice bearing human hepatocellular carcinoma xenografts with high metastatic potential". Molecular Medicine Reports 13, no. 3 (2016): 2518-2526. https://doi.org/10.3892/mmr.2016.4854
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