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March-2016 Volume 13 Issue 3

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Enhancer of zeste homolog 2 depletion arrests the proliferation of hepatoblastoma cells

  • Authors:
    • Yue Wang
    • Yongtao Xiao
    • Kai Chen
    • Sheng Chen
    • Min Zhang
    • Zhixiang Wu
    • Yeming Wu
  • View Affiliations / Copyright

    Affiliations: Department of Pediatric Surgery, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, P.R. China, Shanghai Institute for Pediatric Research, Shanghai 200092, P.R. China, Department of Pediatric Surgery, Shanghai Children's Medical Center, Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200127, P.R. China
  • Pages: 2724-2728
    |
    Published online on: February 4, 2016
       https://doi.org/10.3892/mmr.2016.4864
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Abstract

Hepatoblastoma is the most common type of malignant liver tumor in children. While outcomes have been greatly improved in the past decades, the treatment of advanced hepatoblastoma has remained challenging. Enhancer of zeste homologue 2 (EZH2), a member of the polycomb group regulators of gene activity, is amplified and overexpressed in a variety of cancers. However, the role of EZH2 in hepatoblastoma has remained to be fully elucidated. The purpose of the present study was to investigate the expression patterns of EZH2 in hepatoblastoma cells and to assess the anti‑cancer effects of EZH2 depletion. Western blot analysis revealed that EZH2 expression was significantly higher in hepatoblastoma specimens compared with that in peri‑tumor tissues, while p27 was reduced in hepatoblastoma. Suppression of EHZ2 using lentiviral small hairpin RNA inhibited hepatoblastoma cell proliferation, induced cell cycle arrest in G1 phase and enhanced the expression of G1/S‑phase checkpoint protein p27. These results suggested that EZH2 may represent a potential diagnostic marker and therapeutic target for the treatment of hepatoblastoma.
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1 

Yang SS, Brough AJ and Bernstein J: Tumors of the liver in early infancy: Hepatoblastoma. Mich Med. 71:539–543. 1972.PubMed/NCBI

2 

Rowland JM: Hepatoblastoma: Assessment of criteria for histologic classification. Med Pediatr Oncol. 39:478–483. 2002. View Article : Google Scholar : PubMed/NCBI

3 

Malogolowkin MH, Katzenstein HM, Krailo M and Meyers RL: Treatment of hepatoblastoma: The North American cooperative group experience. Front Biosci (Elite Ed). 4:1717–1723. 2012. View Article : Google Scholar

4 

Warmann SW and Fuchs J: Drug resistance in hepatoblastoma. Curr Pharm Biotechnol. 8:93–97. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Cao R, Wang L, Wang H, Xia L, Erdjument-Bromage H, Tempst P, Jones RS and Zhang Y: Role of histone H3 lysine 27 methylation in Polycomb-group silencing. Science. 298:1039–1043. 2002. View Article : Google Scholar : PubMed/NCBI

6 

Xiao Y: Enhancer of zeste homolog 2: A potential target for tumor therapy. Int J Biochem Cell Biol. 43:474–477. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Varambally S, Dhanasekaran SM, Zhou M, Barrette TR, Kumar-Sinha C, Sanda MG, Ghosh D, Pienta KJ, Sewalt RG, Otte AP, et al: The polycomb group protein EZH2 is involved in progression of prostate cancer. Nature. 419:624–629. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Chen Y, Lin MC, Yao H, Wang H, Zhang AQ, Yu J, Hui CK, Lau GK, He ML, Sung J and Kung HF: Lentivirus-mediated RNA interference targeting enhancer of zeste homolog 2 inhibits hepatocellular carcinoma growth through down-regulation of stathmin. Hepatology. 46:200–208. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Cai MY, Tong ZT, Zheng F, Liao YJ, Wang Y, Rao HL, Chen YC, Wu QL, Liu YH, Guan XY, et al: EZH2 protein: A promising immunomarker for the detection of hepatocellular carcinomas in liver needle biopsies. Gut. 60:967–976. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Hajósi-Kalcakosz S, Dezső K, Bugyik E, Bödör C, Paku S, Pávai Z, Halász J, Schlachter K, Schaff Z and Nagy P: Enhancer of zeste homologue 2 (EZH2) is a reliable immunohistochemical marker to differentiate malignant and benign hepatic tumors. Diagn Pathol. 7:862012. View Article : Google Scholar : PubMed/NCBI

11 

Yonemitsu Y, Imazeki F, Chiba T, Fukai K, Nagai Y, Miyagi S, Arai M, Aoki R, Miyazaki M, Nakatani Y, et al: Distinct expression of polycomb group proteins EZH2 and BMI1 in hepatocellular carcinoma. Hum Pathol. 40:1304–1311. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Ougolkov AV, Bilim VN and Billadeau DD: Regulation of pancreatic tumor cell proliferation and chemoresistance by the histone methyltransferase enhancer of zeste homologue 2. Clin Cancer Res. 14:6790–6796. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Kotake Y, Cao R, Viatour P, Sage J, Zhang Y and Xiong Y: pRB family proteins are required for H3K27 trimethylation and Polycomb repression complexes binding to and silencing p16INK4alpha tumor suppressor gene. Genes Dev. 21:49–54. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Fujii S, Ito K, Ito Y and Ochiai A: Enhancer of zeste homologue 2 (EZH2) down-regulates RUNX3 by increasing histone H3 methylation. J Biol Chem. 283:17324–17332. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Nakagawa S, Okabe H, Sakamoto Y, Hayashi H, Hashimoto D, Yokoyama N, Sakamoto K, Kuroki H, Mima K, Nitta H, et al: Enhancer of zeste homolog 2 (EZH2) promotes progression of cholangiocarcinoma cells by regulating cell cycle and apoptosis. Ann Surg Oncol. 20(Suppl 3): S667–S675. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Sakurai T, Bilim VN, Ugolkov AV, Yuuki K, Tsukigi M, Motoyama T and Tomita Y: The enhancer of zeste homolog 2 (EZH2), a potential therapeutic target, is regulated by miR-101 in renal cancer cells. Biochem Biophys Res Commun. 422:607–614. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Xiao Y, Chen K, Chen S, Zhang M, Wu Z and Wu Y: Enhancer of zeste homolog 2 depletion arrests the proliferation of hepatoblastoma cells. Mol Med Rep 13: 2724-2728, 2016.
APA
Wang, Y., Xiao, Y., Chen, K., Chen, S., Zhang, M., Wu, Z., & Wu, Y. (2016). Enhancer of zeste homolog 2 depletion arrests the proliferation of hepatoblastoma cells. Molecular Medicine Reports, 13, 2724-2728. https://doi.org/10.3892/mmr.2016.4864
MLA
Wang, Y., Xiao, Y., Chen, K., Chen, S., Zhang, M., Wu, Z., Wu, Y."Enhancer of zeste homolog 2 depletion arrests the proliferation of hepatoblastoma cells". Molecular Medicine Reports 13.3 (2016): 2724-2728.
Chicago
Wang, Y., Xiao, Y., Chen, K., Chen, S., Zhang, M., Wu, Z., Wu, Y."Enhancer of zeste homolog 2 depletion arrests the proliferation of hepatoblastoma cells". Molecular Medicine Reports 13, no. 3 (2016): 2724-2728. https://doi.org/10.3892/mmr.2016.4864
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Xiao Y, Chen K, Chen S, Zhang M, Wu Z and Wu Y: Enhancer of zeste homolog 2 depletion arrests the proliferation of hepatoblastoma cells. Mol Med Rep 13: 2724-2728, 2016.
APA
Wang, Y., Xiao, Y., Chen, K., Chen, S., Zhang, M., Wu, Z., & Wu, Y. (2016). Enhancer of zeste homolog 2 depletion arrests the proliferation of hepatoblastoma cells. Molecular Medicine Reports, 13, 2724-2728. https://doi.org/10.3892/mmr.2016.4864
MLA
Wang, Y., Xiao, Y., Chen, K., Chen, S., Zhang, M., Wu, Z., Wu, Y."Enhancer of zeste homolog 2 depletion arrests the proliferation of hepatoblastoma cells". Molecular Medicine Reports 13.3 (2016): 2724-2728.
Chicago
Wang, Y., Xiao, Y., Chen, K., Chen, S., Zhang, M., Wu, Z., Wu, Y."Enhancer of zeste homolog 2 depletion arrests the proliferation of hepatoblastoma cells". Molecular Medicine Reports 13, no. 3 (2016): 2724-2728. https://doi.org/10.3892/mmr.2016.4864
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