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Article

Electroacupuncture improves cognitive function through Rho GTPases and enhances dendritic spine plasticity in rats with cerebral ischemia‑reperfusion

  • Authors:
    • Ruhui Lin
    • Yunan Wu
    • Jing Tao
    • Bin Chen
    • Jixiang Chen
    • Congkuai Zhao
    • Kunqiang Yu
    • Xiaojie Li
    • Li‑Dian Chen
  • View Affiliations / Copyright

    Affiliations: College of Rehabilitation Medicine, Fujian University of Traditional Medicine, Fuzhou, Fujian 350122, P.R. China, TCM Rehabilitation Research Center of SATCM, Fujian University of Traditional Medicine, Fuzhou, Fujian 350122, P.R. China, Fujian Key Laboratory of Exercise Rehabilitation, Fujian University of Traditional Medicine, Fuzhou, Fujian 350122, P.R. China, Fujian Rehabilitation Tech Co‑innovation Center, Fujian University of Traditional Medicine, Fuzhou, Fujian 350122, P.R. China
  • Pages: 2655-2660
    |
    Published online on: February 5, 2016
       https://doi.org/10.3892/mmr.2016.4870
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Abstract

The aim of the present study was to evaluate the effect of electroacupuncture (EA) on cognitive function following cerebral ischemia‑reperfusion (I/R) injury, and to clarify the mechanism through which Rho GTPase is associated with EA analgesia modulation of dendritic spine plasticity. Rats were randomly divided into three groups: The sham surgery group, the middle cerebral artery occlusion (MCAO) model of ischemia group, and the MCAO with EA (MCAO+EA) treatment group. The MCAO+EA group received treatment with EA at points of Baihui (DU20) and Shenting (DU24) following surgery. It was demonstrated that treatment with EA significantly (P<0.05) protected the cognitive function of rats from impairment caused by cerebral I/R injury. Furthermore, EA treatment increased the density of dendritic spines in the hippocampus of cerebral I/R‑injured rats. Simultaneously, EA increased the expression of cell division cycle 42, Ras‑related C3 botulinum toxin substrate 1 and F‑actin proteins. By contrast, EA treatment inhibited the expression of Ras homologous member A. Collectively, these findings suggest that Rho GTPases and dendritic spine plasticity are critical in mediating the effects of EA treatment at the points of Shenting and Baihui, and that EA protects against impairment of cognitive function following ischemic stroke.
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View References

1 

Go AS, Mozaffarian D, Roger VL, Benjamin EJ, Berry JD, Borden WB, Bravata DM, Dai S, Ford ES, Fox CS, et al: Heart disease and stroke statistics-2013 update: A report from the American heart association. Circulation. 127:e6–e245. 2013. View Article : Google Scholar

2 

Shim H: Vascular cognitive impairment and post-stroke cognitive deficits. Curr Neurol Neurosci Rep. 14:4182014. View Article : Google Scholar

3 

Béjot Y and Giroud M: Mean age at stroke onset: An instructive tool from epidemiological studies. Eur J Neurol. 16:e32009. View Article : Google Scholar

4 

Douiri A, Rudd AG and Wolfe CD: Prevalence of poststroke cognitive impairment: South London stroke register 1995–2010. Stroke. 44:138–145. 2013. View Article : Google Scholar

5 

Feng X, Yang S, Liu J, Huang J, Peng J, Lin J, Tao J and Chen L: Electroacupuncture ameliorates cognitive impairment through inhibition of NF-κ-mediated neuronal cell apoptosis in cerebral ischemia-reperfusion injured rats. Mol Med Rep. 7:1516–1522. 2013.PubMed/NCBI

6 

Zhang H, Zhao L, Yang S, Chen Z, Li Y, Peng X, Yang Y and Zhu M: Clinical observation on effect of scalp electroacupuncture for mild cognitive impairment. J Tradit Chin Med. 33:46–50. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Zhao L, Zhang H, Zheng Z and Huang J: Electroacupuncture on the head points for improving gnosia in patients with vascular dementia. J Tradit Chin Med. 29:29–34. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Chen Y, Zhou J, Li J, Yang SB, Mo LQ, Hu JH and Yuan WL: Electroacupuncture pretreatment prevents cognitive impairment induced by limb ischemia-reperfusion via inhibition of microglial activation and attenuation of oxidative stress in rats. Brain Res. 1432:36–45. 2012. View Article : Google Scholar

9 

Govek EE, Hatten ME and Van Aelst L: The role of Rho GTPase proteins in CNS neuronal migration. Dev Neurobiol. 71:528–553. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Matus A: Actin-based plasticity in dendritic spines. Science. 290:754–758. 2000. View Article : Google Scholar : PubMed/NCBI

11 

Newey SE, Velamoor V, Govek EE and Van Aelst L: Rho GTPases, dendritic structure and mental retardation. J Neurobiol. 64:58–74. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Bosch M and Hayashi Y: Structural plasticity of dendritic spines. Curr Opin Neurobiol. 22:383–388. 2012. View Article : Google Scholar

13 

Vadodaria KC, Brakebusch C, Suter U and Jessberger S: Stage-specific functions of the small Rho GTPases Cdc42 and Rac1 for adult hippocampal neurogenesis. J Neurosci. 33:1179–1189. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Georges PC, Hadzimichalis NM, Sweet ES and Firestein BL: The yin-yang of dendrite morphology: Unity of actin and microtubules. Mol Neurobiol. 38:270–284. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Kasai H, Fukuda M, Watanabe S, Hayashi-Takagi A and Noguchi J: Structural dynamics of dendritic spines in memory and cognition. Trends Neurosci. 33:121–129. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Rodriguez GA, Burns MP, Weeber EJ and Rebeck GW: Young APOE4 targeted replacement mice exhibit poor spatial learning and memory, with reduced dendritic spine density in the medial entorhinal cortex. Learn Mem. 20:256–266. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Longa EZ, Weinstein PR, Carlson S and Cummins R: Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke. 20:84–91. 1989. View Article : Google Scholar : PubMed/NCBI

18 

Koyama Y and Tohyama M: A modified and highly sensitive Golgi-Cox method to enable complete and stable impregnation of embryonic neurons. J Neurosci Methods. 209:58–61. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Jacquin A, Binquet C, Rouaud O, Graule-Petot A, Daubail B, Osseby GV, Bonithon-Kopp C, Giroud M and Béjot Y: Post-stroke cognitive impairment: High prevalence and determining factors in a cohort of mild stroke. J Alzheimers Dis. 40:1029–1038. 2014.PubMed/NCBI

20 

Thrift AG, Dewey HM, Macdonell RA, McNeil JJ and Donnan GA: Incidence of the major stroke subtypes: Initial findings from the north east Melbourne stroke incidence study (NEMESIS). Stroke. 32:1732–1738. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Chen L, Fang J, Ma R, Froym R, Gu X, Li J, Chen L, Xu S and Ji C: Acupuncture for acute stroke: Study protocol for a multi-center, randomized, controlled trial. Trials. 15:2142014. View Article : Google Scholar

22 

Liu F, Li ZM, Jiang YJ and Chen LD: A meta-analysis of acupuncture use in the treatment of cognitive impairment after stroke. J Alternat Complement Med. 20:535–544. 2014. View Article : Google Scholar

23 

Deng W, Aimone JB and Gage FH: New neurons and new memories: How does adult hippocampal neurogenesis affect learning and memory? Nat Rev Neurosci. 11:339–350. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Manns JR and Eichenbaum H: A cognitive map for object memory in the hippocampus. Learn Mem. 16:616–624. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Eyre MD, Richter-Levin G, Avital A and Stewart MG: Morphological changes in hippocampal dentate gyrus synapses following spatial learning in rats are transient. Eur J Neurosci. 17:1973–1980. 2003. View Article : Google Scholar : PubMed/NCBI

26 

Halpain S, Spencer K and Graber S: Dynamics and pathology of dendritic spines. Prog Brain Res. 147:29–37. 2005. View Article : Google Scholar

27 

Govek EE, Newey SE and Van Aelst L: The role of the Rho GTPases in neuronal development. Genes Dev. 19:1–49. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Babayan AH and Kramár EA: Rapid effects of oestrogen on synaptic plasticity: Interactions with actin and its signaling proteins. J Neuroendocrinol. 25:1163–1172. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Santos Da Silva J, Schubert V and Dotti CG: RhoA, Rac1 and cdc42 intracellular distribution shift during hippocampal neuron development. Mol Cell Neurosci. 27:1–7. 2004. View Article : Google Scholar : PubMed/NCBI

30 

Murakoshi H, Wang H and Yasuda R: Local, persistent activation of Rho GTPases during plasticity of single dendritic spines. Nature. 472:100–104. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Auer M, Hausott B and Klimaschewski L: Rho GTPases as regulators of morphological neuroplasticity. Ann Anat. 193:259–266. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Iden S and Collard JG: Crosstalk between small GTPases and polarity proteins in cell polarization. Nat Rev Mol Cell Biol. 9:846–859. 2008. View Article : Google Scholar : PubMed/NCBI

33 

Edwards DC, Sanders LC, Bokoch GM and Gill GN: Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase signalling to actin cytoskeletal dynamics. Nat Cell Biol. 1:253–259. 1999. View Article : Google Scholar : PubMed/NCBI

34 

Meng Y, Zhang Y, Tregoubov V, Janus C, Cruz L, Jackson M, Lu WY, MacDonald JF, Wang JY, Falls DL and Jia Z: Abnormal spine morphology and enhanced LTP in LIMK-1 knockout mice. Neuron. 35:121–133. 2002. View Article : Google Scholar : PubMed/NCBI

35 

Sun ico CR, González-Forero D, Dom ínguez G, García-Verdugo JM and Moreno-López B: Nitric oxide induces pathological synapse loss by a protein kinase G-, Rho kinase-dependent mechanism preceded by myosin light chain phosphorylation. J Neurosci. 30:973–984. 2010. View Article : Google Scholar

36 

Aburima A, Wraith KS, Raslan Z, Law R, Magwenzi S and Naseem KM: cAMP signaling regulates platelet myosin light chain (MLC) phosphorylation and shape change through targeting the RhoA-Rho kinase-MLC phosphatase signaling pathway. Blood. 122:3533–3545. 2013. View Article : Google Scholar : PubMed/NCBI

37 

Pilpel Y and Segal M: Activation of PKC induces rapid morphological plasticity in dendrites of hippocampal neurons via Rac and Rho-dependent mechanisms. Eur J Neurosci. 19:3151–3164. 2004. View Article : Google Scholar : PubMed/NCBI

38 

Lin X, Ogiya M, Takahara M, Yamaguchi W, Furuyama T, Tanaka H, Tohyama M and Inagaki S: Sema4D-plexin-B1 implicated in regulation of dendritic spine density through RhoA/ROCK pathway. Neurosci Lett. 428:1–6. 2007. View Article : Google Scholar : PubMed/NCBI

39 

Tashiro A, Minden A and Yuste R: Regulation of dendritic spine morphology by the rho family of small GTPases: Antagonistic roles of Rac and Rho. Cereb Cortex. 10:927–938. 2000. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Lin R, Wu Y, Tao J, Chen B, Chen J, Zhao C, Yu K, Li X and Chen LD: Electroacupuncture improves cognitive function through Rho GTPases and enhances dendritic spine plasticity in rats with cerebral ischemia‑reperfusion. Mol Med Rep 13: 2655-2660, 2016.
APA
Lin, R., Wu, Y., Tao, J., Chen, B., Chen, J., Zhao, C. ... Chen, L. (2016). Electroacupuncture improves cognitive function through Rho GTPases and enhances dendritic spine plasticity in rats with cerebral ischemia‑reperfusion. Molecular Medicine Reports, 13, 2655-2660. https://doi.org/10.3892/mmr.2016.4870
MLA
Lin, R., Wu, Y., Tao, J., Chen, B., Chen, J., Zhao, C., Yu, K., Li, X., Chen, L."Electroacupuncture improves cognitive function through Rho GTPases and enhances dendritic spine plasticity in rats with cerebral ischemia‑reperfusion". Molecular Medicine Reports 13.3 (2016): 2655-2660.
Chicago
Lin, R., Wu, Y., Tao, J., Chen, B., Chen, J., Zhao, C., Yu, K., Li, X., Chen, L."Electroacupuncture improves cognitive function through Rho GTPases and enhances dendritic spine plasticity in rats with cerebral ischemia‑reperfusion". Molecular Medicine Reports 13, no. 3 (2016): 2655-2660. https://doi.org/10.3892/mmr.2016.4870
Copy and paste a formatted citation
x
Spandidos Publications style
Lin R, Wu Y, Tao J, Chen B, Chen J, Zhao C, Yu K, Li X and Chen LD: Electroacupuncture improves cognitive function through Rho GTPases and enhances dendritic spine plasticity in rats with cerebral ischemia‑reperfusion. Mol Med Rep 13: 2655-2660, 2016.
APA
Lin, R., Wu, Y., Tao, J., Chen, B., Chen, J., Zhao, C. ... Chen, L. (2016). Electroacupuncture improves cognitive function through Rho GTPases and enhances dendritic spine plasticity in rats with cerebral ischemia‑reperfusion. Molecular Medicine Reports, 13, 2655-2660. https://doi.org/10.3892/mmr.2016.4870
MLA
Lin, R., Wu, Y., Tao, J., Chen, B., Chen, J., Zhao, C., Yu, K., Li, X., Chen, L."Electroacupuncture improves cognitive function through Rho GTPases and enhances dendritic spine plasticity in rats with cerebral ischemia‑reperfusion". Molecular Medicine Reports 13.3 (2016): 2655-2660.
Chicago
Lin, R., Wu, Y., Tao, J., Chen, B., Chen, J., Zhao, C., Yu, K., Li, X., Chen, L."Electroacupuncture improves cognitive function through Rho GTPases and enhances dendritic spine plasticity in rats with cerebral ischemia‑reperfusion". Molecular Medicine Reports 13, no. 3 (2016): 2655-2660. https://doi.org/10.3892/mmr.2016.4870
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