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Article Open Access

Increased expression of dermatopontin and its implications for testicular dysfunction in mice

  • Authors:
    • Jun Cai
    • Weijia Liu
    • Jie Hao
    • Maoxin Chen
    • Gang Li
  • View Affiliations / Copyright

    Affiliations: Institute of Life Sciences, Chongqing Medical University, Chongqing 400016, P.R. China, Experimental Research Center, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, P.R. China
    Copyright: © Cai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2431-2438
    |
    Published online on: February 5, 2016
       https://doi.org/10.3892/mmr.2016.4879
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Abstract

An array of specific and non‑specific molecules, which are expressed in the testis, have been demonstrated to be responsible for testicular function. Our previous study revealed that dermatopontin (DPT) is expressed in Sertoli cells of the testis, however, its roles in testicular function remains somewhat elusive. In the present study, CdCl2‑ and busulfan‑induced testicular dysfunction models were used to investigate the implications of DPT expression for testicular function. The mRNA and protein expression levels of DPT were detected using reverse transcription‑quantitative polymerase chain reaction and western blotting, respectively. A negative correlation was observed between testicular damage and the expression of DPT, which suggested that an increase in DPT expression may be a marker for testicular dysfunction. This result was corroborated by the finding that transgenic mice exhibiting Sertoli cell‑specific overexpression of DPT exhibited damage to their testicular morphology. Additionally, DPT overexpression in the testis affected the expression levels of claudin‑11 and zonula occludens‑1, which indicated that DPT may affect testicular function by affecting the integrity of the blood‑testis barrier (BTB). In conclusion, the present study provided evidence to suggest that DPT may be indicative of mouse testicular dysfunction, since increased expression may be associated with damage to the BTB.
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Copy and paste a formatted citation
Spandidos Publications style
Cai J, Liu W, Hao J, Chen M and Li G: Increased expression of dermatopontin and its implications for testicular dysfunction in mice. Mol Med Rep 13: 2431-2438, 2016.
APA
Cai, J., Liu, W., Hao, J., Chen, M., & Li, G. (2016). Increased expression of dermatopontin and its implications for testicular dysfunction in mice. Molecular Medicine Reports, 13, 2431-2438. https://doi.org/10.3892/mmr.2016.4879
MLA
Cai, J., Liu, W., Hao, J., Chen, M., Li, G."Increased expression of dermatopontin and its implications for testicular dysfunction in mice". Molecular Medicine Reports 13.3 (2016): 2431-2438.
Chicago
Cai, J., Liu, W., Hao, J., Chen, M., Li, G."Increased expression of dermatopontin and its implications for testicular dysfunction in mice". Molecular Medicine Reports 13, no. 3 (2016): 2431-2438. https://doi.org/10.3892/mmr.2016.4879
Copy and paste a formatted citation
x
Spandidos Publications style
Cai J, Liu W, Hao J, Chen M and Li G: Increased expression of dermatopontin and its implications for testicular dysfunction in mice. Mol Med Rep 13: 2431-2438, 2016.
APA
Cai, J., Liu, W., Hao, J., Chen, M., & Li, G. (2016). Increased expression of dermatopontin and its implications for testicular dysfunction in mice. Molecular Medicine Reports, 13, 2431-2438. https://doi.org/10.3892/mmr.2016.4879
MLA
Cai, J., Liu, W., Hao, J., Chen, M., Li, G."Increased expression of dermatopontin and its implications for testicular dysfunction in mice". Molecular Medicine Reports 13.3 (2016): 2431-2438.
Chicago
Cai, J., Liu, W., Hao, J., Chen, M., Li, G."Increased expression of dermatopontin and its implications for testicular dysfunction in mice". Molecular Medicine Reports 13, no. 3 (2016): 2431-2438. https://doi.org/10.3892/mmr.2016.4879
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