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Article

Characterization of the malaria parasite protein PfTip, a novel invasion-related protein

  • Authors:
    • Xuewu Liu
    • Yuxiao Huang
    • Jiao Liang
    • Jun Wang
    • Yan Shen
    • Yinghui Li
    • Ya Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Pathogenic Biology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China
  • Pages: 3303-3310
    |
    Published online on: February 18, 2016
       https://doi.org/10.3892/mmr.2016.4910
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Abstract

Malaria is one of the most common infective diseases in the world. Invasion of host erythrocytes by the malaria parasite is crucial for pathogen survival and pathogenesis. Various proteins mediate parasite invasion and identification of novel invasion-related proteins may aid in elucidating the underlying molecular mechanism and new intervention strategies for malaria control. This study characterized the PfTip protein, a homolog of the human T‑cell immunomodulatory protein, and examined its function in preventing parasite infection. Bioinformatics analysis and experimental validation were adopted in the present study. Bioinformatics analysis showed that PfTip has a β‑propeller fold in its structure and is highly expressed at the early ring stage. TNFRSF14 was predicted to be a candidate interactant of PfTip. Further analyses showed that PfTip blockage by sera inhibited erythrocyte invasion by the malaria parasite. The protective effect of PfTip was further confirmed through in vivo analysis. To the best of our knowledge, this study is the first to provide evidence on the function of PfTip in erythrocyte parasite invasion. Additional assays involving the receptor of this protein are currently underway.
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Copy and paste a formatted citation
Spandidos Publications style
Liu X, Huang Y, Liang J, Wang J, Shen Y, Li Y and Zhao Y: Characterization of the malaria parasite protein PfTip, a novel invasion-related protein. Mol Med Rep 13: 3303-3310, 2016.
APA
Liu, X., Huang, Y., Liang, J., Wang, J., Shen, Y., Li, Y., & Zhao, Y. (2016). Characterization of the malaria parasite protein PfTip, a novel invasion-related protein. Molecular Medicine Reports, 13, 3303-3310. https://doi.org/10.3892/mmr.2016.4910
MLA
Liu, X., Huang, Y., Liang, J., Wang, J., Shen, Y., Li, Y., Zhao, Y."Characterization of the malaria parasite protein PfTip, a novel invasion-related protein". Molecular Medicine Reports 13.4 (2016): 3303-3310.
Chicago
Liu, X., Huang, Y., Liang, J., Wang, J., Shen, Y., Li, Y., Zhao, Y."Characterization of the malaria parasite protein PfTip, a novel invasion-related protein". Molecular Medicine Reports 13, no. 4 (2016): 3303-3310. https://doi.org/10.3892/mmr.2016.4910
Copy and paste a formatted citation
x
Spandidos Publications style
Liu X, Huang Y, Liang J, Wang J, Shen Y, Li Y and Zhao Y: Characterization of the malaria parasite protein PfTip, a novel invasion-related protein. Mol Med Rep 13: 3303-3310, 2016.
APA
Liu, X., Huang, Y., Liang, J., Wang, J., Shen, Y., Li, Y., & Zhao, Y. (2016). Characterization of the malaria parasite protein PfTip, a novel invasion-related protein. Molecular Medicine Reports, 13, 3303-3310. https://doi.org/10.3892/mmr.2016.4910
MLA
Liu, X., Huang, Y., Liang, J., Wang, J., Shen, Y., Li, Y., Zhao, Y."Characterization of the malaria parasite protein PfTip, a novel invasion-related protein". Molecular Medicine Reports 13.4 (2016): 3303-3310.
Chicago
Liu, X., Huang, Y., Liang, J., Wang, J., Shen, Y., Li, Y., Zhao, Y."Characterization of the malaria parasite protein PfTip, a novel invasion-related protein". Molecular Medicine Reports 13, no. 4 (2016): 3303-3310. https://doi.org/10.3892/mmr.2016.4910
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