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Article

Astragaloside IV suppresses inflammatory mediator production in synoviocytes and collagen‑induced arthritic rats

  • Authors:
    • Hao Xu
    • Chang‑Yao Wang
    • Hai‑Ning Zhang
    • Cheng‑Yu Lv
    • Ying‑Zhen Wang
  • View Affiliations / Copyright

    Affiliations: Joint Department of Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, Shandong 266100, P.R. China
  • Pages: 3289-3296
    |
    Published online on: February 22, 2016
       https://doi.org/10.3892/mmr.2016.4923
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Abstract

The aim of the current study was to investigate the effects of Astragaloside‑IV (AS‑IV) on inflammatory mediators in synoviocytes and collagen‑induced arthritic rats. Synoviocytes were stimulated with lipopolysaccharides (LPS) and Sprague‑Dawley rats were injected with type II collagen. AS‑IV was administered to the LPS‑stimulated synoviocytes and collagen‑induced arthritis (CIA) rats. The inflammation of LPS‑stimulated synoviocytes and CIA rats was assessed using enzyme‑linked immunosorbent assays and western blotting. Using Cell Counting Kit‑8 analysis, it was demonstrated that AS‑IV (5, 20 and 50 mg/ml) inhibited the LPS‑stimulated synoviocytes proliferation in a dose‑dependent manner. AS‑IV significantly inhibited the LPS‑stimulated inflammatory response, as indicated by the expression levels of TNF‑α, IL‑1β, IL‑6 and IL‑8. In addition, treatment with AS‑IV significantly reduced the LPS‑stimulated cyclooxygenase (COX)‑1, COX‑2, high mobility group box 1 protein (HMGB1) and intercellular adhesion molecule 1 overexpression, and intranuclear nuclear factor (NF)‑κBp65 subunit accumulation and activation of c‑Jun N‑terminal kinase (JNK)1/2 and p38. Similar to the protective effects of AS‑IV on LPS‑stimulated synoviocytes, AS‑IV treatment significantly reduced the expression levels of tumor necrosis factor α, interleukin (IL)‑1β, IL‑6 and IL‑8 expression levels, and attenuated intranuclear NF‑κBp65 subunit accumulation and overexpression of COX‑2 and inducible nitric oxide synthase in CIA rats. In conclusion, the results of the present study demonstrated for the first time, to the best of our knowledge, that AS‑IV protects synoviocytes against LPS‑ and collagen‑induced inflammatory responses through inhibition of the HMGB1‑dependent JNK1/2‑ and p38‑activated NF‑κB/COX‑2 pathway.
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View References

1 

Davignon JL, Hayder M, Baron M, Boyer JF, Constantin A, Apparailly F, Poupot R and Cantagrel A: Targeting monocytes/macrophages in the treatment of rheumatoid arthritis. Rheumatology (Oxford). 52:590–598. 2013. View Article : Google Scholar

2 

Walter GJ, Evans HG, Menon B, Gullick NJ, Kirkham BW, Cope AP, Geissmann F and Taams LS: Interaction with activated monocytes enhances cytokine expression and suppressive activity of human CD4 CD45RO CD25 CD127low regulatory T cells. Arthritis Rheum. 65:627–638. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Bianchi R, Giambanco I and Donato R: S100B/RAGE-dependent activation of microglia via NF-κB and AP-1: Co-regulation of COX-2 expression by S100B, IL-1β and TNF-α. Neurobiol Aging. 31:665–677. 2010. View Article : Google Scholar

4 

Salminen A, Huuskonen J, Ojala J, Kauppinen A, Kaarniranta K and Suuronen T: Activation of innate immunity system during aging: NF-κB signaling is the molecular culprit of inflamm-aging. Ageing Res Rev. 7:83–105. 2008. View Article : Google Scholar

5 

Sheeba M and Asha V: Cardiospermum halicacabum ethanol extract inhibits LPS induced COX-2, TNF-α and iNOS expression, which is mediated by NF-κB regulation, in RAW264.7 cells. J Ethnopharmacol. 124:39–44. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Kumar S, Singhal V, Roshan R, Sharma A, Rembhotkar GW and Ghosh B: Piperine inhibits TNF-α induced adhesion of neutrophils to endothelial monolayer through suppression of NF-κB and IκB kinase activation. Eur J Pharmacol. 575:177–186. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Wang B: Anti-arthritic effect of astragaloside IV and its molecular mechanism. ICS. 12014.Review.

8 

Ma XQ, Shi Q, Duan J, Dong TT and Tsim KW: Chemical analysis of Radix Astragali (Huangqi) in China: A comparison with its adulterants and seasonal variations. J Agric Food Chem. 50:4861–4866. 2002. View Article : Google Scholar : PubMed/NCBI

9 

Lei H, Wang B, Li WP, Yang Y, Zhou AW and Chen MZ: Anti-aging effect of astragalosides and its mechanism of action. Acta Pharmacol Sin. 24:230–234. 2003.PubMed/NCBI

10 

Yu QT, Qi LW, Li P, Yi L, Zhao J and Bi Z: Determination of seventeen main flavonoids and saponins in the medicinal plant Huang-qi (Radix Astragali) by HPLC-DAD-ELSD. J Sep Sci. 30:1292–1299. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Cho WCS and Leung KN: In vitro and in vivo immunomodulating and immunorestorative effects of Astragalus membranaceus. J Ethnopharmacol. 113:132–141. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Chan WS, Durairajan SSK, Lu JH, Wang Y, Xie LX, Kum WF, Koo I, Yung KKL and Li M: Neuroprotective effects of Astragaloside IV in 6-hydroxydopamine-treated primary nigral cell culture. Neurochem Int. 55:414–422. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Zhang WD, Chen H, Zhang C, Liu RH, Li HL and Chen HZ: Astragaloside IV from Astragalus membranaceus shows cardioprotection during myocardial ischemia in vivo and in vitro. Planta Med. 72:4–8. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Nagy G, Koncz A, Telarico T, Fernandez D, Érsek B, Buzás E and Perl A: Central role of nitric oxide in the pathogenesis of rheumatoid arthritis and sysemic lupus erythematosus. Arthritis Res Ther. 12:2102010. View Article : Google Scholar :

15 

Schett G, Coates LC, Ash ZR, Finzel S and Conaghan PG: Structural damage in rheumatoid arthritis, psoriatic arthritis, and ankylosing spondylitis: Traditional views, novel insights gained from TNF blockade, and concepts for the future. Arthritis Res Ther. 13:S42011.PubMed/NCBI

16 

Wang B and Chen MZ: Astragaloside IV possesses antiarthritic effect by preventing interleukin 1β-induced joint inflammation and cartilage damage. Arch Pharm Res. 37:793–802. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Zhu L, Wei W, Zheng YQ and Jia XY: Effects and mechanisms of total glucosides of paeony on joint damage in rat collagen-induced arthritis. Inflamm Res. 54:211–220. 2005. View Article : Google Scholar : PubMed/NCBI

18 

McInnes IB and Schett G: The pathogenesis of rheumatoid arthritis. N Engl J Med. 365:2205–2219. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Takaishi S, Okumura T, Tu S, Wang SSW, Shibata W, Vigneshwaran R, Gordon SAK, Shimada Y and Wang TC: Identification of gastric cancer stem cells using the cell surface marker CD44. Stem Cells. 27:1006–1020. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Lee Y, Hwang J, Lee H, Cheon YJ, Ryu JH, Lee SI, Kwak HB, Lee SM, Kim JS, Park JW, et al: SPA0355, a thiourea analogue, inhibits inflammatory responses and joint destruction in fibroblast-like synoviocytes and mice with collagen-induced arthritis. Br J Pharmacol. 164:794–806. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Han I, Jeong SJ, Lee HJ, Koh W, Lee HJ, Lee EO, Kim HS, Lee SJ, Chen CY, Jung MH and Kim SH: Proteomic analysis of mesenchymal stem-like cells derived from ovarian teratoma: Potential role of glutathione S-transferase M2 in ovarian teratoma. Proteomics. 11:352–360. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Paramalingam SS, Thumboo J, Vasoo S, Thio ST, Tse C and Fong K-Y: In vivo pro-and anti-inflammatory cytokines in normal and patients with rheumatoid arthritis. Ann Acad Med Singap. 36:962007.

23 

Katchamart W, Trudeau J, Phumethum V and Bombardier C: The efficacy and toxicity of methotrexate (MTX) monotherapy versus MTX combination therapy with non-biological disease-modifying anti-rheumatic drugs in rheumatoid arthritis: A systematic review and meta-analysis. Ann Rheum Dis. 68:1105–1112. 2009. View Article : Google Scholar

24 

Simon L and Yocum D: New and future drug therapies for rheumatoid arthritis. Rheumatology (Oxford). 39(Suppl 1): 36–42. 2000. View Article : Google Scholar

25 

Xin W, Huang C, Zhang X, Xin S, Zhou Y, Ma X, Zhang D, Li Y, Zhou S, Zhang D, et al: Methyl salicylate lactoside inhibits inflammatory response of fibroblast-like synoviocytes and joint destruction in collagen-induced arthritis in mice. Br J Pharmacol. 171:3526–3538. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Yang C, Yang Z, Zhang M, Dong Q, Wang X, Lan A, Zeng F, Chen P, Wang C and Feng J: Hydrogen sulfide protects against chemical hypoxia-induced cytotoxicity and inflammation in HaCaT cells through inhibition of ROS/NF-κB/COX-2 pathway. PLoS One. 6:e219712011. View Article : Google Scholar

27 

Tsutakawa S, Kobayashi D, Kusama M, Moriya T and Nakahata N: Nicotine enhances skin necrosis and expression of inflammatory mediators in a rat pressure ulcer model. Br J Dermatol. 161:1020–1027. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Ha MK, Song YH, Jeong SJ, Lee HJ, Jung JH, Kim B, Song HS, Huh JE and Kim SH: Emodin inhibits proinflammatory responses and inactivates histone deacetylase 1 in hypoxic rheumatoid synoviocytes. Biol Pharm Bull. 34:1432–1437. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Peng C, Perera PK, Li YM, Fang WR, Liu LF and Li FW: Anti-inflammatory effects of Clematis chinensis Osbeck extract (AR-6) may be associated with NF-κB, TNF-α, and COX-2 in collagen-induced arthritis in rat. Rheumatol Int. 32:3119–3125. 2012. View Article : Google Scholar

30 

Hah YS, Lee YR, Jun JS, Lim H-S, Kim HO, Jeong YG, Hur GM, Lee SY, Chung MJ, Park JW, et al: A20 suppresses inflammatory responses and bone destruction in human fibroblast-like synoviocytes and in mice with collagen-induced arthritis. Arthritis Rheum. 62:2313–2321. 2010. View Article : Google Scholar : PubMed/NCBI

31 

García-Arnandis I, Guillén MI, Gomar F, Pelletier JP, Martel-Pelletier J and Alcaraz MJ: High mobility group box 1 potentiates the pro-inflammatory effects of interleukin-1β in osteoarthritic synoviocytes. Arthritis Res Ther. 12:R1652010. View Article : Google Scholar

32 

Loeser RF, Yammani RR, Carlson CS, Chen H, Cole A, Im HJ, Bursch LS and Yan SD: Articular chondrocytes express the receptor for advanced glycation end products: Potential role in osteoarthritis. Arthritis Rheum. 52:2376–2385. 2005. View Article : Google Scholar : PubMed/NCBI

33 

Zhang HG, Wang Y, Xie JF, Liang X, Liu D, Yang P, Hsu HC, Ray RB and Mountz JD: Regulation of tumor necrosis factor alpha-mediated apoptosis of rheumatoid arthritis synovial fibroblasts by the protein kinase Akt. Arthritis Rheum. 44:1555–1567. 2001. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xu H, Wang CY, Zhang HN, Lv CY and Wang YZ: Astragaloside IV suppresses inflammatory mediator production in synoviocytes and collagen‑induced arthritic rats. Mol Med Rep 13: 3289-3296, 2016.
APA
Xu, H., Wang, C., Zhang, H., Lv, C., & Wang, Y. (2016). Astragaloside IV suppresses inflammatory mediator production in synoviocytes and collagen‑induced arthritic rats. Molecular Medicine Reports, 13, 3289-3296. https://doi.org/10.3892/mmr.2016.4923
MLA
Xu, H., Wang, C., Zhang, H., Lv, C., Wang, Y."Astragaloside IV suppresses inflammatory mediator production in synoviocytes and collagen‑induced arthritic rats". Molecular Medicine Reports 13.4 (2016): 3289-3296.
Chicago
Xu, H., Wang, C., Zhang, H., Lv, C., Wang, Y."Astragaloside IV suppresses inflammatory mediator production in synoviocytes and collagen‑induced arthritic rats". Molecular Medicine Reports 13, no. 4 (2016): 3289-3296. https://doi.org/10.3892/mmr.2016.4923
Copy and paste a formatted citation
x
Spandidos Publications style
Xu H, Wang CY, Zhang HN, Lv CY and Wang YZ: Astragaloside IV suppresses inflammatory mediator production in synoviocytes and collagen‑induced arthritic rats. Mol Med Rep 13: 3289-3296, 2016.
APA
Xu, H., Wang, C., Zhang, H., Lv, C., & Wang, Y. (2016). Astragaloside IV suppresses inflammatory mediator production in synoviocytes and collagen‑induced arthritic rats. Molecular Medicine Reports, 13, 3289-3296. https://doi.org/10.3892/mmr.2016.4923
MLA
Xu, H., Wang, C., Zhang, H., Lv, C., Wang, Y."Astragaloside IV suppresses inflammatory mediator production in synoviocytes and collagen‑induced arthritic rats". Molecular Medicine Reports 13.4 (2016): 3289-3296.
Chicago
Xu, H., Wang, C., Zhang, H., Lv, C., Wang, Y."Astragaloside IV suppresses inflammatory mediator production in synoviocytes and collagen‑induced arthritic rats". Molecular Medicine Reports 13, no. 4 (2016): 3289-3296. https://doi.org/10.3892/mmr.2016.4923
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