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Article

Effect of transforming growth factor-β3 on the expression of Smad3 and Smad7 in tenocytes

  • Authors:
    • Ke Jiang
    • Guo Chun
    • Ziming Wang
    • Quanyin Du
    • Aimin Wang
    • Yan Xiong
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China, Department of Medicine, Luohe Medical College, Luohe, Henan 462002, P.R. China, Department of Orthopedics, Daping Hospital, The Third Military Medical University, Chongqing 400042, P.R. China
  • Pages: 3567-3573
    |
    Published online on: February 29, 2016
       https://doi.org/10.3892/mmr.2016.4944
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Abstract

Tendon adhesion is a common problem in the healing of injured tendons. The molecular mechanisms of the TGF-β/Smad signaling pathway have been determined, and the role of TGF-β has been well characterized in wound healing. However, the intracellular mechanism or downstream signals by which TGF-β3 modulates its effects on tendon healing have not been well elucidated. The aim of this study was to determine the effect of TGF‑β3 on the TGF-β/Smad signaling pathway in tenocytes. Quantitative polymerase chain reaction and western blot analysis were used to analyze the effect of TGF‑β3 on the regulation of the expression of Smad proteins in tenocytes. The results demonstrated that TGF‑β3 has no significant effect on the proliferation of tendon cells. The addition of TGF‑β3 to tenocytes can significantly downregulate the expression of Smad3 and upregulate the expression of Smad7 at the gene and protein levels. The results demonstrate that TGF‑β3 may regulate Smad3 and Smad7 proteins through the TGF-β/Smad signaling pathway to minimize extrinsic scarring. Thus, it may provide a novel approach to decrease tendon adhesion and promote tendon healing.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang K, Chun G, Wang Z, Du Q, Wang A and Xiong Y: Effect of transforming growth factor-β3 on the expression of Smad3 and Smad7 in tenocytes. Mol Med Rep 13: 3567-3573, 2016.
APA
Jiang, K., Chun, G., Wang, Z., Du, Q., Wang, A., & Xiong, Y. (2016). Effect of transforming growth factor-β3 on the expression of Smad3 and Smad7 in tenocytes. Molecular Medicine Reports, 13, 3567-3573. https://doi.org/10.3892/mmr.2016.4944
MLA
Jiang, K., Chun, G., Wang, Z., Du, Q., Wang, A., Xiong, Y."Effect of transforming growth factor-β3 on the expression of Smad3 and Smad7 in tenocytes". Molecular Medicine Reports 13.4 (2016): 3567-3573.
Chicago
Jiang, K., Chun, G., Wang, Z., Du, Q., Wang, A., Xiong, Y."Effect of transforming growth factor-β3 on the expression of Smad3 and Smad7 in tenocytes". Molecular Medicine Reports 13, no. 4 (2016): 3567-3573. https://doi.org/10.3892/mmr.2016.4944
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang K, Chun G, Wang Z, Du Q, Wang A and Xiong Y: Effect of transforming growth factor-β3 on the expression of Smad3 and Smad7 in tenocytes. Mol Med Rep 13: 3567-3573, 2016.
APA
Jiang, K., Chun, G., Wang, Z., Du, Q., Wang, A., & Xiong, Y. (2016). Effect of transforming growth factor-β3 on the expression of Smad3 and Smad7 in tenocytes. Molecular Medicine Reports, 13, 3567-3573. https://doi.org/10.3892/mmr.2016.4944
MLA
Jiang, K., Chun, G., Wang, Z., Du, Q., Wang, A., Xiong, Y."Effect of transforming growth factor-β3 on the expression of Smad3 and Smad7 in tenocytes". Molecular Medicine Reports 13.4 (2016): 3567-3573.
Chicago
Jiang, K., Chun, G., Wang, Z., Du, Q., Wang, A., Xiong, Y."Effect of transforming growth factor-β3 on the expression of Smad3 and Smad7 in tenocytes". Molecular Medicine Reports 13, no. 4 (2016): 3567-3573. https://doi.org/10.3892/mmr.2016.4944
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