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April-2016 Volume 13 Issue 4

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Article

Comparative proteomic analysis of heat stress proteins associated with rat sperm maturation

  • Authors:
    • Xiaomei Wang
    • Fujun Liu
    • Xin Gao
    • Xin Liu
    • Xiaojun Kong
    • Haiyan Wang
    • Jianyuan Li
  • View Affiliations / Copyright

    Affiliations: Central Laboratory, Yantai Yu Huang Ding Hospital, Yantai, Shandong 264000, P.R. China, College of Life Science, Yantai University, Yantai, Shandong 264005, P.R. China
  • Pages: 3547-3552
    |
    Published online on: March 2, 2016
       https://doi.org/10.3892/mmr.2016.4958
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Abstract

Heat stress is demonstrated to have an effect on the function of the male testis, however, limited information has been reported on its effects on sperm maturation. In the present study, a comparative proteomic analysis was performed on the rat caput epididymal fluids responsible for sperm maturation, to identify key heat‑stress‑associated sperm maturation proteins. The results demonstrated 21 proteins corresponding to 29 differential protein spots, including 10 downregulated and 11 upregulated proteins in the heat treatment group. Functional analysis demonstrated that these proteins were primarily involved in enriched reproduction and antioxidant activity. Analysis of western blot and immunohistochemical analysis demonstrated that the expression of antioxidant proteins peroxiredoxin 6 and clusterin were downregulated, and the expression of superoxide dismutase upregulated, in the heat treatment group. Morphological and TUNEL experiments demonstrated that altered nucleus activity occurred in the caput epididymis. The study provided, to the best of our knowledge, novel information for studies on the biological functions of the epididymis and sperm maturation.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Liu F, Gao X, Liu X, Kong X, Wang H and Li J: Comparative proteomic analysis of heat stress proteins associated with rat sperm maturation. Mol Med Rep 13: 3547-3552, 2016.
APA
Wang, X., Liu, F., Gao, X., Liu, X., Kong, X., Wang, H., & Li, J. (2016). Comparative proteomic analysis of heat stress proteins associated with rat sperm maturation. Molecular Medicine Reports, 13, 3547-3552. https://doi.org/10.3892/mmr.2016.4958
MLA
Wang, X., Liu, F., Gao, X., Liu, X., Kong, X., Wang, H., Li, J."Comparative proteomic analysis of heat stress proteins associated with rat sperm maturation". Molecular Medicine Reports 13.4 (2016): 3547-3552.
Chicago
Wang, X., Liu, F., Gao, X., Liu, X., Kong, X., Wang, H., Li, J."Comparative proteomic analysis of heat stress proteins associated with rat sperm maturation". Molecular Medicine Reports 13, no. 4 (2016): 3547-3552. https://doi.org/10.3892/mmr.2016.4958
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Liu F, Gao X, Liu X, Kong X, Wang H and Li J: Comparative proteomic analysis of heat stress proteins associated with rat sperm maturation. Mol Med Rep 13: 3547-3552, 2016.
APA
Wang, X., Liu, F., Gao, X., Liu, X., Kong, X., Wang, H., & Li, J. (2016). Comparative proteomic analysis of heat stress proteins associated with rat sperm maturation. Molecular Medicine Reports, 13, 3547-3552. https://doi.org/10.3892/mmr.2016.4958
MLA
Wang, X., Liu, F., Gao, X., Liu, X., Kong, X., Wang, H., Li, J."Comparative proteomic analysis of heat stress proteins associated with rat sperm maturation". Molecular Medicine Reports 13.4 (2016): 3547-3552.
Chicago
Wang, X., Liu, F., Gao, X., Liu, X., Kong, X., Wang, H., Li, J."Comparative proteomic analysis of heat stress proteins associated with rat sperm maturation". Molecular Medicine Reports 13, no. 4 (2016): 3547-3552. https://doi.org/10.3892/mmr.2016.4958
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