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SENP‑1 enhances hypoxia‑induced proliferation of rat pulmonary artery smooth muscle cells by regulating hypoxia‑inducible factor‑1α

  • Authors:
    • Fang Zhou
    • Aiguo Dai
    • Yongliang Jiang
    • Xiaowu Tan
    • Xiufeng Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Hunan Institute of Gerontology, Hunan Province Geriatric Hospital, Changsha, Hunan 410016, P.R. China, Department of Respiratory Medicine, The Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3482-3490
    |
    Published online on: March 3, 2016
       https://doi.org/10.3892/mmr.2016.4969
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Abstract

Hypoxic pulmonary vascular remodeling (HPSR) has an important role in the development of hypoxic pulmonary hypertension. HPSR is predominantly mediated by the proliferation of pulmonary artery smooth muscle cells (PASMCs). Our previous study demonstrated that hypoxia‑inducible factor (HIF)‑1α was able to promote the proliferation of PASMCs. Small ubiquitin‑like modifier (SUMO)ylation is a post‑translational modification that is important in various cellular processes. It has previously been demonstrated that HIF‑1α may be SUMOylated by SUMO. Conversely, SUMO‑specific protease 1 (SENP‑1) was able to increase the stability of HIF‑1α by decreasing SUMOylation of HIF‑1α. In order to investigate whether SUMOylation of HIF‑1α has a role in the proliferation of PASMCs, the present study cultured PASMCs in hypoxic and normoxic chambers in vitro. The proliferation ability of PASMCs was measured using the Cell Counting kit‑8 and 5‑ethynyl‑2'‑deoxyuridine cell proliferation assays. In addition, short hairpin RNA lentiviral particles were used to knockdown the expression of SENP‑1, and the expression levels of HIF‑1α, SENP‑1 and vascular endothelial growth factor (VEGF) were detected at the mRNA and protein levels using semi‑quantitative polymerase chain reaction and western blotting, respectively. The present study demonstrated that SENP‑1 was able to enhance the proliferative ability of PASMCs by initiating deSUMOylation of HIF‑1α and increasing the expression of its downstream responsive gene, VEGF.
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1 

Vestbo J, Hurd SS, Agusti AG, Jones PW, Vogelmeier C, Anzueto A, Barnes PJ, Fabbri LM, Martinez FJ, Nishimura M, et al: Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease: GOLD executive summary. Am J Respir Crit Care Med. 187:347–365. 2013. View Article : Google Scholar

2 

Wright JL, Levy RD and Churg A: Pulmonary hypertension in chronic obstructive pulmonary disease: Current theories of pathogenesis and their implications for treatment. Thorax. 60:605–609. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Jin H, Wang Y, Zhou L, Liu L, Zhang P, Deng W and Yuan Y: Melatonin attenuates hypoxic pulmonary hypertension by inhibiting the inflammation and the proliferation of pulmonary arterial smooth muscle cells. J Pineal Res. 57:442–450. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Sarkar J, Gou D, Turaka P, Viktorova E, Ramchandran R and Raj JU: MicroRNA-21 plays a role in hypoxia-mediated pulmonary artery smooth muscle cell proliferation and migration. Am J Physiol Lung Cell Mol Physiol. 299:L861–L871. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Chen B, Calvert AE, Cui H and Nelin LD: Hypoxia promotes human pulmonary artery smooth muscle cell proliferation through induction of arginase. Am J Physiol Lung Cell Mol Physiol. 297:L1151–L1159. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Growcott EJ, Banner KH and Wharton J: Hypoxia amplifies the proliferative capacity of distal human pulmonary artery smooth-muscle cells. Chest. 128(6 Suppl): 600S–601S. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Li QF and Dai AG: Hypoxia-inducible factor-1 alpha regulates the role of vascular endothelial growth factor on pulmonary arteries of rats with hypoxia-induced pulmonary hypertension. Chin Med J (Engl). 117:1023–1028. 2004.

8 

Li QF and Dai AG: Hypoxia inducible factor-1 alpha correlates the expression of heme oxygenase 1 gene in pulmonary arteries of rat with hypoxia-induced pulmonary hypertension. Acta Biochim Biophys Sin (Shanghai). 36:133–140. 2004. View Article : Google Scholar

9 

Zhang R, Wu Y, Zhao M, Liu C, Zhou L, Shen S, Liao S, Yang K, Li Q and Wan H: Role of HIF-1alpha in the regulation ACE and ACE2 expression in hypoxic human pulmonary artery smooth muscle cells. Am J Physiol Lung Cell Mol Physiol. 297:L631–L640. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Rey S and Semenza GL: Hypoxia-inducible factor-1-dependent mechanisms of vascularization and vascular remodelling. Cardiovasc Res. 86:236–242. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Saitoh H and Hinchey J: Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3. J Biol Chem. 275:6252–6258. 2000. View Article : Google Scholar : PubMed/NCBI

12 

Liu B and Shuai K: Regulation of the sumoylation system in gene expression. Curr Opin Cell Biol. 20:288–293. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Yee Koh M, Spivak-Kroizman TR and Powis G: HIF-1 regulation: Not so easy come, easy go. Trends Biochem Sci. 33:526–534. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Xu Y, Zuo Y, Zhang H, Kang X, Yue F, Yi Z, Liu M, Yeh ET, Chen G and Cheng J: Induction of SENP1 in endothelial cells contributes to hypoxia-driven VEGF expression and angiogenesis. J Biol Chem. 285:36682–36688. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Hickey CM, Wilson NR and Hochstrasser M: Function and regulation of SUMO proteases. Nat Rev Mol Cell Biol. 13:755–766. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Gu J, Fan Y, Liu X, Zhou L, Cheng J, Cai R and Xue S: SENP1 protects against myocardial ischaemia/reperfusion injury via a HIF1α-dependent pathway. Cardiovasc Res. 104:83–92. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Wang J, Shimoda LA and Sylvester JT: Capacitative calcium entry and TRPC channel proteins are expressed in rat distal pulmonary arterial smooth muscle. Am J Physiol Lung Cell Mol Physiol. 286:L848–L858. 2004. View Article : Google Scholar

18 

Semenza GL: Hypoxia-inducible factor 1: Control of oxygen homeostasis in health and disease. Pediatr Res. 49:614–617. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Ortiz-Barahona A, Villar D, Pescador N, Amigo J and del Peso L: Genome-wide identification of hypoxia-inducible factor binding sites and target genes by a probabilistic model integrating transcription-profiling data and in silico binding site prediction. Nucleic Acids Res. 38:2332–2345. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Mole DR, Blancher C, Copley RR, Pollard PJ, Gleadle JM, Ragoussis J and Ratcliffe PJ: Genome-wide association of hypoxia-inducible factor (HIF)-1alpha and HIF-2alpha DNA binding with expression profiling of hypoxia-inducible transcripts. J Biol Chem. 284:16767–16775. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Oladipupo S, Hu S, Kovalski J, Yao J, Santeford A, Sohn RE, Shohet R, Maslov K, Wang LV and Arbeit JM: VEGF is essential for hypoxia-inducible factor-mediated neovascularization but dispensable for endothelial sprouting. Proc Natl Acad Sci USA. 108:13264–13269. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Cucina A, Borrelli V, Randone B, Coluccia P, Sapienza P and Cavallaro A: Vascular endothelial growth factor increases the migration and proliferation of smooth muscle cells through the mediation of growth factors released by endothelial cells. J Surg Res. 109:16–23. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Bae SH, Jeong JW, Park JA, Kim SH, Bae MK, Choi SJ and Kim KW: Sumoylation increases HIF-1alpha stability and its transcriptional activity. Biochem Biophys Res Commun. 324:394–400. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Carbia-Nagashima A, Gerez J, Perez-Castro C, Paez-Pereda M, Silberstein S, Stalla GK, Holsboer F and Arzt E: RSUME, a small RWD-containing protein, enhances SUMO conjugation and stabilizes HIF-1alpha during hypoxia. Cell. 131:309–323. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Berta MA, Mazure N, Hattab M, Pouysségur J and Brahimi-Horn MC: SUMOylation of hypoxia-inducible factor-1alpha reduces its transcriptional activity. Biochem Biophys Res Commun. 360:646–652. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Cheng J, Kang X, Zhang S and Yeh ET: SUMO-specific protease 1 is essential for stabilization of HIF1alpha during hypoxia. Cell. 131:584–595. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Yeh ET: SUMOylation and De-SUMOylation: Wrestling with life's processes. J Biol Chem. 284:8223–8227. 2009. View Article : Google Scholar :

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Copy and paste a formatted citation
Spandidos Publications style
Zhou F, Dai A, Jiang Y, Tan X and Zhang X: SENP‑1 enhances hypoxia‑induced proliferation of rat pulmonary artery smooth muscle cells by regulating hypoxia‑inducible factor‑1α. Mol Med Rep 13: 3482-3490, 2016.
APA
Zhou, F., Dai, A., Jiang, Y., Tan, X., & Zhang, X. (2016). SENP‑1 enhances hypoxia‑induced proliferation of rat pulmonary artery smooth muscle cells by regulating hypoxia‑inducible factor‑1α. Molecular Medicine Reports, 13, 3482-3490. https://doi.org/10.3892/mmr.2016.4969
MLA
Zhou, F., Dai, A., Jiang, Y., Tan, X., Zhang, X."SENP‑1 enhances hypoxia‑induced proliferation of rat pulmonary artery smooth muscle cells by regulating hypoxia‑inducible factor‑1α". Molecular Medicine Reports 13.4 (2016): 3482-3490.
Chicago
Zhou, F., Dai, A., Jiang, Y., Tan, X., Zhang, X."SENP‑1 enhances hypoxia‑induced proliferation of rat pulmonary artery smooth muscle cells by regulating hypoxia‑inducible factor‑1α". Molecular Medicine Reports 13, no. 4 (2016): 3482-3490. https://doi.org/10.3892/mmr.2016.4969
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou F, Dai A, Jiang Y, Tan X and Zhang X: SENP‑1 enhances hypoxia‑induced proliferation of rat pulmonary artery smooth muscle cells by regulating hypoxia‑inducible factor‑1α. Mol Med Rep 13: 3482-3490, 2016.
APA
Zhou, F., Dai, A., Jiang, Y., Tan, X., & Zhang, X. (2016). SENP‑1 enhances hypoxia‑induced proliferation of rat pulmonary artery smooth muscle cells by regulating hypoxia‑inducible factor‑1α. Molecular Medicine Reports, 13, 3482-3490. https://doi.org/10.3892/mmr.2016.4969
MLA
Zhou, F., Dai, A., Jiang, Y., Tan, X., Zhang, X."SENP‑1 enhances hypoxia‑induced proliferation of rat pulmonary artery smooth muscle cells by regulating hypoxia‑inducible factor‑1α". Molecular Medicine Reports 13.4 (2016): 3482-3490.
Chicago
Zhou, F., Dai, A., Jiang, Y., Tan, X., Zhang, X."SENP‑1 enhances hypoxia‑induced proliferation of rat pulmonary artery smooth muscle cells by regulating hypoxia‑inducible factor‑1α". Molecular Medicine Reports 13, no. 4 (2016): 3482-3490. https://doi.org/10.3892/mmr.2016.4969
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