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In vivo treatment of rat arterial adventitia with interleukin‑1β induces intimal proliferation via the JAK2/STAT3 signaling pathway

  • Authors:
    • Xiao Wang
    • Lihua Chen
    • Jie Liu
    • Tao Yan
    • Gangyong Wu
    • Yang Xia
    • Gangjun Zong
    • Fengsheng Li
  • View Affiliations / Copyright

    Affiliations: Department of Cardiovascular Diseases, The 101st Hospital of PLA, Wuxi, Jiangsu 214044, P.R. China, Department of Radiology, The 101st Hospital of PLA, Wuxi, Jiangsu 214044, P.R. China, Central Laboratories, The Second Artillery General Hospital, Beijing 100088, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3451-3458
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    Published online on: March 7, 2016
       https://doi.org/10.3892/mmr.2016.4982
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Abstract

Previous studies have indicated that adventitial inflammation is involved in the development of atherosclerosis. The aim of this study was to investigate the effect of arterial adventitia inflammation induced by interleukin (IL)‑1β on intimal proliferation and the mechanisms involved in this process. The left common carotid artery adventitia of male rats in the experimental and control groups (25 rats/group) was wrapped with agar containing or without a sustained‑release suspension of 2.5 µg IL‑1β, respectively. Five animals in each group were randomly selected for sacrifice at 2 h, 8 h, 24 h, 48 h, and 1 week post‑treatment. Hematoxylin and eosin staining was performed for to analyze the morphology of the adventitia. The expression of janus kinase (JAK)2, signal transducer and activator of transcription (STAT)3, phosphorylated (p‑)JAK2 and p‑STAT3 were detected by western blot analysis or immunohistochemistry staining. A model of adventitial inflammation was successfully created by wrapping IL‑1β around the rat carotid artery. IL‑1β treatment induced vascular smooth muscle cell proliferation and migration as well as intimal proliferation. In addition, the expression of p‑JAK2 and p‑STAT3 increased after IL‑1β treatment. Furthermore, an inhibitor of JAK2/STAT3 pathway, AG490, suppressed IL‑1β‑induced intimal proliferation and phosphorylation of JAK2 and STAT3. Thus, the JAK2/STAT3 signaling pathway is involved in intimal proliferation caused by vascular adventitial inflammation. Inhibiting the JAK2/STAT3 signaling pathway may be a novel method for the clinical treatment of artery atherosclerosis.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Chen L, Liu J, Yan T, Wu G, Xia Y, Zong G and Li F: In vivo treatment of rat arterial adventitia with interleukin‑1β induces intimal proliferation via the JAK2/STAT3 signaling pathway. Mol Med Rep 13: 3451-3458, 2016.
APA
Wang, X., Chen, L., Liu, J., Yan, T., Wu, G., Xia, Y. ... Li, F. (2016). In vivo treatment of rat arterial adventitia with interleukin‑1β induces intimal proliferation via the JAK2/STAT3 signaling pathway. Molecular Medicine Reports, 13, 3451-3458. https://doi.org/10.3892/mmr.2016.4982
MLA
Wang, X., Chen, L., Liu, J., Yan, T., Wu, G., Xia, Y., Zong, G., Li, F."In vivo treatment of rat arterial adventitia with interleukin‑1β induces intimal proliferation via the JAK2/STAT3 signaling pathway". Molecular Medicine Reports 13.4 (2016): 3451-3458.
Chicago
Wang, X., Chen, L., Liu, J., Yan, T., Wu, G., Xia, Y., Zong, G., Li, F."In vivo treatment of rat arterial adventitia with interleukin‑1β induces intimal proliferation via the JAK2/STAT3 signaling pathway". Molecular Medicine Reports 13, no. 4 (2016): 3451-3458. https://doi.org/10.3892/mmr.2016.4982
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Chen L, Liu J, Yan T, Wu G, Xia Y, Zong G and Li F: In vivo treatment of rat arterial adventitia with interleukin‑1β induces intimal proliferation via the JAK2/STAT3 signaling pathway. Mol Med Rep 13: 3451-3458, 2016.
APA
Wang, X., Chen, L., Liu, J., Yan, T., Wu, G., Xia, Y. ... Li, F. (2016). In vivo treatment of rat arterial adventitia with interleukin‑1β induces intimal proliferation via the JAK2/STAT3 signaling pathway. Molecular Medicine Reports, 13, 3451-3458. https://doi.org/10.3892/mmr.2016.4982
MLA
Wang, X., Chen, L., Liu, J., Yan, T., Wu, G., Xia, Y., Zong, G., Li, F."In vivo treatment of rat arterial adventitia with interleukin‑1β induces intimal proliferation via the JAK2/STAT3 signaling pathway". Molecular Medicine Reports 13.4 (2016): 3451-3458.
Chicago
Wang, X., Chen, L., Liu, J., Yan, T., Wu, G., Xia, Y., Zong, G., Li, F."In vivo treatment of rat arterial adventitia with interleukin‑1β induces intimal proliferation via the JAK2/STAT3 signaling pathway". Molecular Medicine Reports 13, no. 4 (2016): 3451-3458. https://doi.org/10.3892/mmr.2016.4982
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