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Intervertebral disc regeneration using platelet‑rich plasma‑containing bone marrow‑derived mesenchymal stem cells: A preliminary investigation

  • Authors:
    • Shan‑Zheng Wang
    • Ji‑Yang Jin
    • Yu‑Dong Guo
    • Liang‑Yu Ma
    • Qing Chang
    • Xin‑Gui Peng
    • Fang‑Fang Guo
    • Hai‑Xiang Zhang
    • Xin‑Feng Hu
    • Chen Wang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, Zhongda Hospital, Medical School of Southeast University, Nanjing, Jiangsu 210009, P.R. China, Department of Radiology, Zhongda Hospital, Medical School of Southeast University, Nanjing, Jiangsu 210009, P.R. China, Department of Plastic Surgery, Zhongda Hospital, Medical School of Southeast University, Nanjing, Jiangsu 210009, P.R. China, Department of Orthopaedics, Qidong People's Hospital, Qidong, Jiangsu 226200, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3475-3481
    |
    Published online on: March 7, 2016
       https://doi.org/10.3892/mmr.2016.4983
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Abstract

Platelet‑rich plasma (PRP) is a promising strategy for intervertebral disc degeneration (IDD). However, the short half‑life of growth factors released from PRP cannot continuously stimulate the degenerated discs. Thus, the present study hypothesized that the combined use of PRP and bone marrow‑derived mesenchymal stem cells (BMSCs) may repair the early degenerated discs in the long term for their synergistic reparative effect. In the present study, following the induction of early IDD by annular puncture in rabbits, PRP was prepared and mixed with BMSCs (PRP‑BMSC group) for injection into the early degenerated discs. As controls, phosphate‑buffered saline (PBS; PBS group) and PRP (PRP group) were similarly injected. Rabbits without any intervention served as a control group. At 8 weeks following treatment, histological changes of the injected discs were assessed. Magnetic resonance imaging (MRI) was used to detect the T2‑weighted signal intensity of the targeted discs at weeks 1, 2 and 8 following treatment. Annular puncture resulted in disc narrowing and decreased T2‑weighted signal intensity. At weeks 1 and 3, MRI examinations showed regenerative changes in the PRP‑BMSC group and PRP group, whereas the PBS group exhibited a continuous degenerative process of the discs. At 8 weeks post‑injection, the PRP‑BMSCs induced a statistically significant restoration of discs, as shown by MRI (PRP‑BMSCs, vs.PRP and PBS; P<0.05), which was also confirmed by histological evaluations. Thus, compared with PRP, the administration of PRP‑containing BMSCs resulted in a superior regenerative effect on the early degenerated discs, which may be a promising therapeutic strategy for the restoration of early degenerated discs.
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Copy and paste a formatted citation
Spandidos Publications style
Wang SZ, Jin JY, Guo YD, Ma LY, Chang Q, Peng XG, Guo FF, Zhang HX, Hu XF, Wang C, Wang C, et al: Intervertebral disc regeneration using platelet‑rich plasma‑containing bone marrow‑derived mesenchymal stem cells: A preliminary investigation. Mol Med Rep 13: 3475-3481, 2016.
APA
Wang, S., Jin, J., Guo, Y., Ma, L., Chang, Q., Peng, X. ... Wang, C. (2016). Intervertebral disc regeneration using platelet‑rich plasma‑containing bone marrow‑derived mesenchymal stem cells: A preliminary investigation. Molecular Medicine Reports, 13, 3475-3481. https://doi.org/10.3892/mmr.2016.4983
MLA
Wang, S., Jin, J., Guo, Y., Ma, L., Chang, Q., Peng, X., Guo, F., Zhang, H., Hu, X., Wang, C."Intervertebral disc regeneration using platelet‑rich plasma‑containing bone marrow‑derived mesenchymal stem cells: A preliminary investigation". Molecular Medicine Reports 13.4 (2016): 3475-3481.
Chicago
Wang, S., Jin, J., Guo, Y., Ma, L., Chang, Q., Peng, X., Guo, F., Zhang, H., Hu, X., Wang, C."Intervertebral disc regeneration using platelet‑rich plasma‑containing bone marrow‑derived mesenchymal stem cells: A preliminary investigation". Molecular Medicine Reports 13, no. 4 (2016): 3475-3481. https://doi.org/10.3892/mmr.2016.4983
Copy and paste a formatted citation
x
Spandidos Publications style
Wang SZ, Jin JY, Guo YD, Ma LY, Chang Q, Peng XG, Guo FF, Zhang HX, Hu XF, Wang C, Wang C, et al: Intervertebral disc regeneration using platelet‑rich plasma‑containing bone marrow‑derived mesenchymal stem cells: A preliminary investigation. Mol Med Rep 13: 3475-3481, 2016.
APA
Wang, S., Jin, J., Guo, Y., Ma, L., Chang, Q., Peng, X. ... Wang, C. (2016). Intervertebral disc regeneration using platelet‑rich plasma‑containing bone marrow‑derived mesenchymal stem cells: A preliminary investigation. Molecular Medicine Reports, 13, 3475-3481. https://doi.org/10.3892/mmr.2016.4983
MLA
Wang, S., Jin, J., Guo, Y., Ma, L., Chang, Q., Peng, X., Guo, F., Zhang, H., Hu, X., Wang, C."Intervertebral disc regeneration using platelet‑rich plasma‑containing bone marrow‑derived mesenchymal stem cells: A preliminary investigation". Molecular Medicine Reports 13.4 (2016): 3475-3481.
Chicago
Wang, S., Jin, J., Guo, Y., Ma, L., Chang, Q., Peng, X., Guo, F., Zhang, H., Hu, X., Wang, C."Intervertebral disc regeneration using platelet‑rich plasma‑containing bone marrow‑derived mesenchymal stem cells: A preliminary investigation". Molecular Medicine Reports 13, no. 4 (2016): 3475-3481. https://doi.org/10.3892/mmr.2016.4983
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