miR‑30a‑5p in the tumorigenesis of renal cell carcinoma: A tumor suppressive microRNA

  • Authors:
    • Yifan Li
    • Yuchi Li
    • Duqun Chen
    • Lu Jin
    • Zhengming Su
    • Jiaju Liu
    • Hongfang Duan
    • Xiaoqing Li
    • Zhengyu Qi
    • Min Shi
    • Liangchao Ni
    • Shangqi Yang
    • Yaoting Gui
    • Xiangming Mao
    • Yun Chen
    • Yongqing Lai
  • View Affiliations

  • Published online on: March 21, 2016     https://doi.org/10.3892/mmr.2016.5024
  • Pages: 4085-4094
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Abstract

Renal cell carcinoma (RCC) is the most common type of malignant tumor of the adult kidney and has a poor prognosis. MicroRNAs (miRs) are important in a wide range of biological and pathological processes, including cell differentiation, migration, growth, proliferation, apoptosis and metabolism. The present study aimed to determine the role exerted by miR‑30a‑5p in the tumorigenesis of RCC. The expression levels of miR‑30a‑5p in RCC tissues and RCC‑derived cells were demonstrated to be significantly downregulated by real‑time quantitative polymerase chain reaction (RT‑qPCR). Wound scratch assay, cell proliferation assay and flow cytometric analysis revealed that the abilities of migration and proliferation of the RCC‑derived cells were suppressed, whereas cell apoptosis was promoted, when miR‑30a‑5p was overexpressed in these cells. N‑acetylgalactosaminyltransferase 7 (GALNT7) was predicted to be one target gene of miR‑30a‑5p by bioinformatics analysis. Luciferase reporter assay, RT‑qPCR and western blotting were performed to confirm that GALNT7 is the direct conserved target of miR‑30a‑5p. These results suggested that miR‑30a‑5p has a tumor‑suppressive role in the tumorigenesis of RCC.
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May-2016
Volume 13 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Li Y, Li Y, Chen D, Jin L, Su Z, Liu J, Duan H, Li X, Qi Z, Shi M, Shi M, et al: miR‑30a‑5p in the tumorigenesis of renal cell carcinoma: A tumor suppressive microRNA. Mol Med Rep 13: 4085-4094, 2016
APA
Li, Y., Li, Y., Chen, D., Jin, L., Su, Z., Liu, J. ... Lai, Y. (2016). miR‑30a‑5p in the tumorigenesis of renal cell carcinoma: A tumor suppressive microRNA. Molecular Medicine Reports, 13, 4085-4094. https://doi.org/10.3892/mmr.2016.5024
MLA
Li, Y., Li, Y., Chen, D., Jin, L., Su, Z., Liu, J., Duan, H., Li, X., Qi, Z., Shi, M., Ni, L., Yang, S., Gui, Y., Mao, X., Chen, Y., Lai, Y."miR‑30a‑5p in the tumorigenesis of renal cell carcinoma: A tumor suppressive microRNA". Molecular Medicine Reports 13.5 (2016): 4085-4094.
Chicago
Li, Y., Li, Y., Chen, D., Jin, L., Su, Z., Liu, J., Duan, H., Li, X., Qi, Z., Shi, M., Ni, L., Yang, S., Gui, Y., Mao, X., Chen, Y., Lai, Y."miR‑30a‑5p in the tumorigenesis of renal cell carcinoma: A tumor suppressive microRNA". Molecular Medicine Reports 13, no. 5 (2016): 4085-4094. https://doi.org/10.3892/mmr.2016.5024