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Article Open Access

Lycopene protects human SH‑SY5Y neuroblastoma cells against hydrogen peroxide‑induced death via inhibition of oxidative stress and mitochondria‑associated apoptotic pathways

  • Authors:
    • Chunsheng Feng
    • Tianfei Luo
    • Shuyan Zhang
    • Kai Liu
    • Yanhong Zhang
    • Yinan Luo
    • Pengfei Ge
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Neurology, First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Neurosurgery, First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Emergent Medicine, People's Hospital of Jilin Province, Changchun, Jilin 130021, P.R. China
    Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4205-4214
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    Published online on: March 28, 2016
       https://doi.org/10.3892/mmr.2016.5056
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Abstract

Oxidative stress, which is characterized by excessive production of reactive oxygen species (ROS), is a common pathway that results in neuronal injury or death due to various types of pathological stress. Although lycopene has been identified as a potent antioxidant, its effect on hydrogen peroxide (H2O2)‑induced neuronal damage remains unclear. In the present study, pretreatment with lycopene was observed to protect SH‑SY5Y neuroblastoma cells against H2O2‑induced death via inhibition of apoptosis resulting from activation of caspase‑3 and translocation of apoptosis inducing factor (AIF) to the nucleus. Furthermore, the over‑produced ROS, as well as the reduced activities of anti‑oxidative enzymes, superoxide dismutase and catalase, were demonstrated to be alleviated by lycopene. Additionally, lycopene counteracted H2O2‑induced mitochondrial dysfunction, which was evidenced by suppression of mitochondrial permeability transition pore opening, attenuation of the decline of the mitochondrial membrane potential, and inhibition of the increase of Bax and decrease of Bcl‑2 levels within the mitochondria. The release of cytochrome c and AIF from the mitochondria was also reduced. These results indicate that lycopene is a potent neuroprotectant against apoptosis, oxidative stress and mitochondrial dysfunction, and could be administered to prevent neuronal injury or death.
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Copy and paste a formatted citation
Spandidos Publications style
Feng C, Luo T, Zhang S, Liu K, Zhang Y, Luo Y and Ge P: Lycopene protects human SH‑SY5Y neuroblastoma cells against hydrogen peroxide‑induced death via inhibition of oxidative stress and mitochondria‑associated apoptotic pathways. Mol Med Rep 13: 4205-4214, 2016.
APA
Feng, C., Luo, T., Zhang, S., Liu, K., Zhang, Y., Luo, Y., & Ge, P. (2016). Lycopene protects human SH‑SY5Y neuroblastoma cells against hydrogen peroxide‑induced death via inhibition of oxidative stress and mitochondria‑associated apoptotic pathways. Molecular Medicine Reports, 13, 4205-4214. https://doi.org/10.3892/mmr.2016.5056
MLA
Feng, C., Luo, T., Zhang, S., Liu, K., Zhang, Y., Luo, Y., Ge, P."Lycopene protects human SH‑SY5Y neuroblastoma cells against hydrogen peroxide‑induced death via inhibition of oxidative stress and mitochondria‑associated apoptotic pathways". Molecular Medicine Reports 13.5 (2016): 4205-4214.
Chicago
Feng, C., Luo, T., Zhang, S., Liu, K., Zhang, Y., Luo, Y., Ge, P."Lycopene protects human SH‑SY5Y neuroblastoma cells against hydrogen peroxide‑induced death via inhibition of oxidative stress and mitochondria‑associated apoptotic pathways". Molecular Medicine Reports 13, no. 5 (2016): 4205-4214. https://doi.org/10.3892/mmr.2016.5056
Copy and paste a formatted citation
x
Spandidos Publications style
Feng C, Luo T, Zhang S, Liu K, Zhang Y, Luo Y and Ge P: Lycopene protects human SH‑SY5Y neuroblastoma cells against hydrogen peroxide‑induced death via inhibition of oxidative stress and mitochondria‑associated apoptotic pathways. Mol Med Rep 13: 4205-4214, 2016.
APA
Feng, C., Luo, T., Zhang, S., Liu, K., Zhang, Y., Luo, Y., & Ge, P. (2016). Lycopene protects human SH‑SY5Y neuroblastoma cells against hydrogen peroxide‑induced death via inhibition of oxidative stress and mitochondria‑associated apoptotic pathways. Molecular Medicine Reports, 13, 4205-4214. https://doi.org/10.3892/mmr.2016.5056
MLA
Feng, C., Luo, T., Zhang, S., Liu, K., Zhang, Y., Luo, Y., Ge, P."Lycopene protects human SH‑SY5Y neuroblastoma cells against hydrogen peroxide‑induced death via inhibition of oxidative stress and mitochondria‑associated apoptotic pathways". Molecular Medicine Reports 13.5 (2016): 4205-4214.
Chicago
Feng, C., Luo, T., Zhang, S., Liu, K., Zhang, Y., Luo, Y., Ge, P."Lycopene protects human SH‑SY5Y neuroblastoma cells against hydrogen peroxide‑induced death via inhibition of oxidative stress and mitochondria‑associated apoptotic pathways". Molecular Medicine Reports 13, no. 5 (2016): 4205-4214. https://doi.org/10.3892/mmr.2016.5056
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