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Article

Time-dependent activity of Na+/H+ exchanger isoform 1 and homeostasis of intracellular pH in astrocytes exposed to CoCl2 treatment

  • Authors:
    • Peng Wang
    • Ling Li
    • Zhenxiang Zhang
    • Quancheng Kan
    • Feng Gao
    • Suyan Chen
  • View Affiliations / Copyright

    Affiliations: Department of Basic Medicine, Nursing College, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Palliative and Hospice Care, The Ninth People's Hospital of Zhengzhou, Zhengzhou, Henan 450053, P.R. China, Clinical Pharmacology Base, Department of Infectious Disease, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Neuroimmunology, Henan Academy of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
  • Pages: 4443-4450
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    Published online on: March 30, 2016
       https://doi.org/10.3892/mmr.2016.5067
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Abstract

Hypoxia causes injury to the central nervous system during stroke and has significant effects on pH homeostasis. Na+/H+ exchanger isoform 1 (NHE1) is important in the mechanisms of hypoxia and intracellular pH (pHi) homeostasis. As a well-established hypoxia-mimetic agent, CoCl2 stabilizes and increases the expression of hypoxia inducible factor‑1α (HIF-1α), which regulates several genes involved in pH balance, including NHE1. However, it is not fully understood whether NHE1 is activated in astrocytes under CoCl2 treatment. In the current study, pHi and NHE activity were analyzed using the pHi‑sensitive dye BCECF‑AM. Using cariporide (an NHE1‑specific inhibitor) and EIPA (an NHE nonspecific inhibitor), the current study demonstrated that it was NHE1, not the other NHE isoforms, that was important in regulating pHi homeostasis in astrocytes during CoCl2 treatment. Additionally, the present study observed that, during the early period of CoCl2 treatment (the first 2 h), NHE1 activity and pHi dropped immediately, and NHE1 mRNA expression was reduced compared with control levels, whereas expression levels of the NHE1 protein had not yet changed. In the later period of CoCl2 treatment, NHE1 activity and pHi significantly increased compared with the control levels, as did the mRNA and protein expression levels of NHE1. Furthermore, the cell viability and injury of astrocytes was not changed during the initial 8 h of CoCl2 treatment; their deterioration was associated with the higher levels of pHi and NHE1 activity. The current study concluded that NHE1 activity and pHi homeostasis are regulated by CoCl2 treatment in a time-dependent manner in astrocytes, and may be responsible for the changes in cell viability and injury observed under hypoxia-mimetic conditions induced by CoCl2 treatment.
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1 

Weimar C, Goertler M, Harms L and Diener HC: Distribution and outcome of symptomatic stenoses and occlusions in patients with acute cerebral ischemia. Arch Neurol. 63:1287–1291. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Yang Z, Zhao TZ, Zou YJ, Zhang JH and Feng H: Hypoxia induces autophagic cell death through hypoxia-inducible factor 1a in microglia. PLoS One. 9:e965092014. View Article : Google Scholar

3 

Wang C, Wang Z, Zhang X, Zhang X, Dong L, Xing Y, Li Y, Liu Z, Chen L, Qiao H, et al: Protection by silibinin against experimental ischemic stroke: Up-regulated pAkt, pmTOR, HIF-1α and Bcl-2, down-regulated Bax, NF-ĸB expression. Neurosci Lett. 529:45–50. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Chang E, O'Donnell ME and Barakat AI: Shear stress and 17beta-estradiol modulate cerebral microvascular endothelial Na-K-Cl cotransporter and Na/H exchanger protein levels. Am J Physiol Cell Physiol. 294:C363–C371. 2008. View Article : Google Scholar

5 

Uria-Avellanal C and Robertson NJ: Na+/H+ exchangers and intracellular pH in perinatal brain injury. Transl Stroke Res. 5:79–98. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Simon RP, Niro M and Gwinn R: Brain acidosis induced by hypercarbic ventilation attenuates focal ischemic injury. J Pharmacol Exp Ther. 267:1428–1431. 1993.PubMed/NCBI

7 

Vannucci RC, Towfighi J, Brucklacher RM and Vannucci SJ: Effect of extreme hypercapnia on hypoxic-ischemic brain damage in the immature rat. Pediatr Res. 49:799–803. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Kendall GS, Robertson NJ, Iwata O, Peebles D and Raivich G: N-methyl-isobutyl-amiloride ameliorates brain injury when commenced before hypoxia ischemia in neonatal mice. Pediatr Res. 59:227–231. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Kaba NK, Schultz J, Law FY, Lefort CT, Martel-Gallegos G, Kim M, Waugh RE, Arreola J and Knauf PA: Inhibition of Na+/H+ exchanger enhances low pH-induced L-selectin shedding and β-2-integrin surface expression in human neutrophils. Am J Physiol Cell Physiol. 295:C1454–C1463. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Counillon L and Pouysségur J: The expanding family of eukaryotic Na(+)/H(+) exchangers. J Biol Chem. 275:1–4. 2000. View Article : Google Scholar : PubMed/NCBI

11 

Orlowski J and Grinstein S: Diversity of the mammalian sodium/proton exchanger SLC9 gene family. Pflugers Arch. 447:549–565. 2004. View Article : Google Scholar

12 

Xue J, Zhou D, Yao H, Gavrialov O, McConnell MJ, Gelb BD and Haddad GG: Novel functional interaction between Na+/H+ exchanger 1 and tyrosine phosphatase SHP-2. Am J Physiol Regul Integr Comp Physiol. 292:R2406–R2416. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Shi Y, Chanana V, Watters JJ, Ferrazzano P and Sun D: Role of sodium/hydrogen exchanger isoform 1 in microglial activation and proinflamatory responses in ischemic brains. J Neurochem. 119:124–135. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Cengiz P, Kleman N, Uluc K, Kendigelen P, Hagemann T, Akture E, Messing A, Ferrazzano P and Sun D: Inhibition of Na+/H+ exchanger isoform 1 is neuroprotective in neonatal hypoxic ischemic brain injury. Antioxid Redox Signal. 14:1803–1813. 2011. View Article : Google Scholar :

15 

Murphy E, Cross H and Steenbergen C: Sodium regulation during ischemia versus reperfusion and its role in injury. Circ Res. 84:1469–1470. 1999. View Article : Google Scholar : PubMed/NCBI

16 

Pedersen SF, O'Donnell ME, Anderson SE and Cala PM: Physiology and pathophysiology of Na+/H+ exchange and Na+-K+-2Cl-cotransport in the heart, brain, and blood. Am J Physiol Regul Integr Comp Physiol. 291:R1–R25. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Rentsch ML, Ossum CG, Hoffmann EK and Pedersen SF: Roles of Na+/H+ exchange in regulation of p38 mitogen-activated protein kinase activity and cell death after chemical anoxia in NIH3T3 fibroblasts. Pflugers Arch. 454:649–662. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Gibson JS, McCartney D, Sumpter J, Fairfax TP, Milner PI, Edwards HL and Wilkins RJ: Rapid effects of hypoxia on H+ homeostasis in articular chondrocytes. Pflugers Arch. 458:1085–1092. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Azzopardi D, Wyatt JS, Cady EB, Delpy DT, Baudin J, Stewart AL, Hope PL, Hamilton PA and Reynolds EO: Prognosis of newborn infants with hypoxic-ischemic brain injury assessed by phosphorus magnetic resonance spectroscopy. Pediatr Res. 25:445–451. 1989. View Article : Google Scholar : PubMed/NCBI

20 

Wakabayashi S, Fafournoux P, Sardet C and Pouysségur J: The Na+/H+ antiporter cytoplasmic domain mediates growth factor signals and controls 'H(+)-sensing'. Proc Natl Acad Sci USA. 89:2424–2428. 1992. View Article : Google Scholar

21 

Luo J, Chen H, Kintner DB, Shull GE and Sun D: Decreased neuronal death in Na+/H+ exchanger isoform 1-null mice after in vitro and in vivo ischemia. J Neurosci. 25:11256–11268. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Park SL, Lee DH, Yoo SE and Jung YS: The effect of Na(+)/H(+) exchanger-1 inhibition by sabiporide on blood-brain barrier dysfunction after ischemia/hypoxia in vivo and in vitro. Brain Res. 1366:189–196. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Valle-Casuso JC, González-Sánchez A, Medina JM and Tabernero A: HIF-1 and c-Src mediate increased glucose uptake induced by endothelin-1 and connexin43 in astrocytes. PLoS One. 7:e324482012. View Article : Google Scholar : PubMed/NCBI

24 

Wang G, Hazra TK, Mitra S, Lee HM and Englander EW: Mitochondrial DNA damage and a hypoxic response are induced by CoCl(2) in rat neuronal PC12 cells. Nucleic Acids Res. 28:2135–2140. 2000. View Article : Google Scholar : PubMed/NCBI

25 

Karovic O, Tonazzini I, Rebola N, Edström E, Lövdahl C, Fredholm BB and Daré E: Toxic effects of cobalt in primary cultures of mouse astrocytes. Similarities with hypoxia and role of HIF-1alpha. Biochem Pharmacol. 73:694–708. 2007. View Article : Google Scholar

26 

Saxena S, Shukla D and Bansal A: Augmentation of aerobic respiration and mitochondrial biogenesis in skeletal muscle by hypoxia preconditioning with cobalt chloride. Toxicol Appl Pharmacol. 264:324–334. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Caltana L, Merelli A, Lazarowski A and Brusco A: Neuronal and glial alterations due to focal cortical hypoxia induced by direct cobalt chloride (CoCl2) brain injection. Neurotox Res. 15:348–358. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Martin LJ, Brambrink AM, Lehmann C, Portera-Cailliau C, Koehler R, Rothstein J and Traystman RJ: Hypoxia-ischemia causes abnormalities in glutamate transporters and death of astroglia and neurons in newborn striatum. Ann Neurol. 42:335–348. 1997. View Article : Google Scholar : PubMed/NCBI

29 

Giffard RG and Swanson RA: Ischemia-induced programmed cell death in astrocytes. Glia. 50:299–306. 2005. View Article : Google Scholar : PubMed/NCBI

30 

Li L, Zhang B, Tao Y, Wang Y, Wei H, Zhao J, Huang R and Pei Z: DL-3-n-butylphthalide protects endothelial cells against oxidative/nitrosative stress, mitochondrial damage and subsequent cell death after oxygen glucose deprivation in vitro. Brain Res. 1290:91–101. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Takano T, Oberheim N, Cotrina ML and Nedergaard M: Astrocytes and ischemic injury. Stroke. 40:S8–S12. 2009. View Article : Google Scholar :

32 

Badawi Y, Ramamoorthy P and Shi H: Hypoxia-inducible factor 1 protects hypoxic astrocytes against glutamate toxicity. ASN Neuro. 4:231–241. 2012. View Article : Google Scholar : PubMed/NCBI

33 

Efrati S, Fishlev G, Bechor Y, Volkov O, Bergan J, Kliakhandler K, Kamiager I, Gal N, Friedman M, Ben-Jacob E and Golan H: Hyperbaric oxygen induces late neuroplasticity in post stroke patients - randomized, prospective trial. PLoS One. 8:e537162013. View Article : Google Scholar :

34 

Björklund O, Shang M, Tonazzini I, Daré E and Fredholm BB: Adenosine A1 and A3 receptors protect astrocytes from hypoxic damage. Eur J Pharmacol. 596:6–13. 2008. View Article : Google Scholar : PubMed/NCBI

35 

McCarthy KD and de Vellis J: Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue. J Cell Biol. 85:890–902. 1980. View Article : Google Scholar : PubMed/NCBI

36 

Kato H, Kogure K, Araki T and Itoyama Y: Astroglial and microglial reactions in the gerbil hippocampus with induced ischemic tolerance. Brain Res. 664:69–76. 1994. View Article : Google Scholar : PubMed/NCBI

37 

Roos A and Boron WF: Intracellular pH. Physiol Rev. 61:296–434. 1981.PubMed/NCBI

38 

Zhao H, Shiue H, Palkon S, Wang Y, Cullinan P, Burkhardt JK, Musch MW, Chang EB and Turner JR: Ezrin regulates NHE3 translocation and activation after Na-glucose cotransport. Proc Natl Acad Sci USA. 101:9485–90. 2004. View Article : Google Scholar

39 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar

40 

Wang Y, Luo J, Chen X, Chen H, Cramer SW and Sun D: Gene inactivation of Na+/H+ exchanger isoform 1 attenuates apoptosis and mitochondrial damage following transient focal cerebral ischemia. Eur J Neurosci. 28:51–61. 2008. View Article : Google Scholar : PubMed/NCBI

41 

Harley W, Floyd C, Dunn T, Zhang XD, Chen TY, Hegde M, Palandoken H, Nantz MH, Leon L, Carraway KL III, et al: Dual inhibition of sodium-mediated proton and calcium efflux triggers non-apoptotic cell death in malignant gliomas. Brain Res. 1363:159–169. 2010. View Article : Google Scholar : PubMed/NCBI

42 

Yu L, Quinn DA, Garg HG and Hales CA: Deficiency of the NHE1 gene prevents hypoxia-induced pulmonary hypertension and vascular remodeling. Am J Respir Crit Care Med. 177:1276–1284. 2008. View Article : Google Scholar : PubMed/NCBI

43 

Cengiz P, Kleman N, Uluc K, Kendigelen P, Hagemann T, Akture E, Messing A, Ferrazzano P and Sun D: Inhibition of Na+/H+ exchanger isoform 1 is neuroprotective in neonatal hypoxic ischemic brain injury. Antioxid Redox Signal. 14:1803–1813. 2011. View Article : Google Scholar :

44 

Wada M, Miyakawa S, Shimada A, Okada N, Yamamoto A and Fujita T: Functional linkage of H+/peptide transporter PEPT2 and Na+/H+ exchanger in primary cultures of astrocytes from mouse cerebral cortex. Brain Res. 1044:33–41. 2005. View Article : Google Scholar : PubMed/NCBI

45 

Orlowski J and Grinstein S: Na+/H+ exchangers of mammalian cells. J Biol Chem. 272:22373–22376. 1997. View Article : Google Scholar : PubMed/NCBI

46 

Son EJ, Moon IS, Kim SH, Kim SJ and Choi JY: Interferon-gamma suppresses Na+-H+ exchanger in cultured human endolymphatic sac epithelial cells. J Cell Biochem. 107:965–972. 2009. View Article : Google Scholar : PubMed/NCBI

47 

Wilkins RJ and Hall AC: Measurement of intracellular pH in isolated bovine articular chondrocytes. Exp Physiol. 77:521–524. 1992. View Article : Google Scholar : PubMed/NCBI

48 

Browning JA and Wilkins RJ: The characterisation of mechanisms regulating intracellular pH in a transformed human articular chondrocyte cell line C-20/A4. J Physiol. 513P:54P1998.

49 

Tattersall AL, Browning JA and Wilkins RJ: Modulation of H+ transport mechanisms by interleukin-1 in isolated bovine articular chondrocytes. Cell Physiol Biochem. 16:43–50. 2005. View Article : Google Scholar

50 

Hand SC: Oxygen, pHi and arrest of biosynthesis in brine shrimp embryos. Acta Physiol Scand. 61:543–551. 1997. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Wang P, Li L, Zhang Z, Kan Q, Gao F and Chen S: Time-dependent activity of Na+/H+ exchanger isoform 1 and homeostasis of intracellular pH in astrocytes exposed to CoCl2 treatment. Mol Med Rep 13: 4443-4450, 2016.
APA
Wang, P., Li, L., Zhang, Z., Kan, Q., Gao, F., & Chen, S. (2016). Time-dependent activity of Na+/H+ exchanger isoform 1 and homeostasis of intracellular pH in astrocytes exposed to CoCl2 treatment. Molecular Medicine Reports, 13, 4443-4450. https://doi.org/10.3892/mmr.2016.5067
MLA
Wang, P., Li, L., Zhang, Z., Kan, Q., Gao, F., Chen, S."Time-dependent activity of Na+/H+ exchanger isoform 1 and homeostasis of intracellular pH in astrocytes exposed to CoCl2 treatment". Molecular Medicine Reports 13.5 (2016): 4443-4450.
Chicago
Wang, P., Li, L., Zhang, Z., Kan, Q., Gao, F., Chen, S."Time-dependent activity of Na+/H+ exchanger isoform 1 and homeostasis of intracellular pH in astrocytes exposed to CoCl2 treatment". Molecular Medicine Reports 13, no. 5 (2016): 4443-4450. https://doi.org/10.3892/mmr.2016.5067
Copy and paste a formatted citation
x
Spandidos Publications style
Wang P, Li L, Zhang Z, Kan Q, Gao F and Chen S: Time-dependent activity of Na+/H+ exchanger isoform 1 and homeostasis of intracellular pH in astrocytes exposed to CoCl2 treatment. Mol Med Rep 13: 4443-4450, 2016.
APA
Wang, P., Li, L., Zhang, Z., Kan, Q., Gao, F., & Chen, S. (2016). Time-dependent activity of Na+/H+ exchanger isoform 1 and homeostasis of intracellular pH in astrocytes exposed to CoCl2 treatment. Molecular Medicine Reports, 13, 4443-4450. https://doi.org/10.3892/mmr.2016.5067
MLA
Wang, P., Li, L., Zhang, Z., Kan, Q., Gao, F., Chen, S."Time-dependent activity of Na+/H+ exchanger isoform 1 and homeostasis of intracellular pH in astrocytes exposed to CoCl2 treatment". Molecular Medicine Reports 13.5 (2016): 4443-4450.
Chicago
Wang, P., Li, L., Zhang, Z., Kan, Q., Gao, F., Chen, S."Time-dependent activity of Na+/H+ exchanger isoform 1 and homeostasis of intracellular pH in astrocytes exposed to CoCl2 treatment". Molecular Medicine Reports 13, no. 5 (2016): 4443-4450. https://doi.org/10.3892/mmr.2016.5067
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