Transfection of chondromodulin I into human breast cancer cells and its effect on the inhibition of cancer cell growth

  • Authors:
    • Jie Shao
    • Linghong Gan
    • Jie Wang
  • View Affiliations

  • Published online on: March 31, 2016     https://doi.org/10.3892/mmr.2016.5079
  • Pages: 4303-4308
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Abstract

Breast cancer affects one in every eight women, and has been associated with higher rates of female mortality than any other cancer type, with the exception of lung cancer. It has been reported that chondromodulin I (ChM-I) was able to suppress tumor angiogenesis and growth in vivo. In order to investigate the antitumor action of ChM‑I on human breast cancer cells, a plasmid expressing ChM‑I was constructed and transfected into human breast cancer cells using an adenoviral vector. Reverse transcription‑polymerase chain reaction detected ChM‑I expression in human breast cancer cell lines, whereas no expression was detected in the control groups. In order to assess the effect of ChM‑I on human breast cancer cells, cell counting kit‑8 (CCK‑8) and colony formation analyses were used to detect tumor cell proliferation, and the proliferation of ChM‑I‑transfected cells was significantly reduced, as compared with the control. In addition, the mRNA expression levels of cell cycle‑associated genes in ChM‑I‑transfected cells were significantly decreased, as compared with the control, which suggested that ChM‑I transfection was able to inhibit the expression of genes associated with the cell cycle. The results of the present study indicated that ChM‑I was able to inhibit the growth of breast cancer cells; thus suggesting that ChM-I may have potential clinical applications in the treatment of breast cancer.
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May-2016
Volume 13 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Shao J, Gan L and Wang J: Transfection of chondromodulin I into human breast cancer cells and its effect on the inhibition of cancer cell growth. Mol Med Rep 13: 4303-4308, 2016
APA
Shao, J., Gan, L., & Wang, J. (2016). Transfection of chondromodulin I into human breast cancer cells and its effect on the inhibition of cancer cell growth. Molecular Medicine Reports, 13, 4303-4308. https://doi.org/10.3892/mmr.2016.5079
MLA
Shao, J., Gan, L., Wang, J."Transfection of chondromodulin I into human breast cancer cells and its effect on the inhibition of cancer cell growth". Molecular Medicine Reports 13.5 (2016): 4303-4308.
Chicago
Shao, J., Gan, L., Wang, J."Transfection of chondromodulin I into human breast cancer cells and its effect on the inhibition of cancer cell growth". Molecular Medicine Reports 13, no. 5 (2016): 4303-4308. https://doi.org/10.3892/mmr.2016.5079