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May-2016 Volume 13 Issue 5

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Article

Transfection of chondromodulin I into human breast cancer cells and its effect on the inhibition of cancer cell growth

  • Authors:
    • Jie Shao
    • Linghong Gan
    • Jie Wang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China, Department of Nursing, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China
  • Pages: 4303-4308
    |
    Published online on: March 31, 2016
       https://doi.org/10.3892/mmr.2016.5079
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Abstract

Breast cancer affects one in every eight women, and has been associated with higher rates of female mortality than any other cancer type, with the exception of lung cancer. It has been reported that chondromodulin I (ChM-I) was able to suppress tumor angiogenesis and growth in vivo. In order to investigate the antitumor action of ChM‑I on human breast cancer cells, a plasmid expressing ChM‑I was constructed and transfected into human breast cancer cells using an adenoviral vector. Reverse transcription‑polymerase chain reaction detected ChM‑I expression in human breast cancer cell lines, whereas no expression was detected in the control groups. In order to assess the effect of ChM‑I on human breast cancer cells, cell counting kit‑8 (CCK‑8) and colony formation analyses were used to detect tumor cell proliferation, and the proliferation of ChM‑I‑transfected cells was significantly reduced, as compared with the control. In addition, the mRNA expression levels of cell cycle‑associated genes in ChM‑I‑transfected cells were significantly decreased, as compared with the control, which suggested that ChM‑I transfection was able to inhibit the expression of genes associated with the cell cycle. The results of the present study indicated that ChM‑I was able to inhibit the growth of breast cancer cells; thus suggesting that ChM-I may have potential clinical applications in the treatment of breast cancer.
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1 

Brinton LA, Smith L, Gierach GL, Pfeiffer RM, Nyante SJ, Sherman ME, Park Y, Hollenbeck AR and Dallal CM: Breast cancer risk in older women: Results from the NIH-AARP diet and health study. Cancer Causes Control. 25:843–857. 2014. View Article : Google Scholar : PubMed/NCBI

2 

National Cancer Institute: Breast cancer. What You Need To Know About. National Institutes of Health; Bethesda, MD: 2012

3 

Nyante SJ, Dallal CM, Gierach GL, Park Y, Hollenbeck AR and Brinton LA: Risk factors for specific histopathological types of postmenopausal breast cancer in the NIH-AARP diet and health study. Am J Epidemiol. 178:359–371. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Sasco AJ: Breast cancer and the environment. Horm Res. 60(Suppl 3): S502003. View Article : Google Scholar

5 

Ruddy KJ, Greaney ML, Sprunck-Harrild K, Meyer ME, Emmons KM and Partridge AH: Young women with breast cancer: A focus group study of unmet needs. J Adolesc Young Adult Oncol. 2:153–160. 2013. View Article : Google Scholar :

6 

John EM, Hopper JL, Beck JC, Knight JA, Neuhausen SL, Senie RT, Ziogas A, Andrulis IL, Anton-Culver H, Boyd N, et al: The breast cancer family registry: An infrastructure for cooperative multinational, interdisciplinary and translational studies of the genetic epidemiology of breast cancer. Breast Cancer Res. 6:R375–R389. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Fantozzi A and Christofori G: Mouse models of breast cancer metastasis. Breast Cancer Res. 8:2122006. View Article : Google Scholar : PubMed/NCBI

8 

Zetter BR: Angiogenesis and tumor metastasis. Annu Rev Med. 49:407–424. 1998. View Article : Google Scholar : PubMed/NCBI

9 

Mera H, Kawashima H, Yoshizawa T, Ishibashi O, Ali MM, Hayami T, Kitahara H, Yamagiwa H, Kondo N, Ogose A, et al: Chondromodulin-1 directly suppresses growth of human cancer cells. BMC Cancer. 9:1662009. View Article : Google Scholar : PubMed/NCBI

10 

Xing S, Wang Z, Xi H, Zhou L, Wang D, Sang L, Wang X, Qi M and Zhai L: Establishment of rat bone mesenchymal stem cell lines stably expressing Chondromodulin I. Int J Clin Exp Med. 5:34–43. 2012.PubMed/NCBI

11 

Lin L, Fu X, Zhang X, Chen LX, Zhang JY, Yu CL, Ma KT and Zhou CY: Rat adipose-derived stromal cells expressing BMP4 induce ectopic bone formation in vitro and in vivo. Acta Pharmacol Sin. 27:1608–1615. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Wang S, Zeng X, Liu Y, Liang C, Zhang H, Liu C, Du W and Zhang Z: Construction and characterization of a PDCD5 recombinant lentivirus vector and its expression in tumor cells. Oncol Rep. 28:91–98. 2012.PubMed/NCBI

13 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar

14 

Zheng K, Li HY, Su XL, Wang XY, Tian T, Li F and Ren GS: Chemokine receptor CXCR7 regulates the invasion, angiogenesis and tumor growth of human hepatocellular carcinoma cells. J Exp Clin Cancer Res. 29:312010. View Article : Google Scholar : PubMed/NCBI

15 

Nishida N, Yano H, Nishida T, Kamura T and Kojiro M: Angiogenesis in cancer. Vasc Health Risk Manag. 2:213–219. 2006. View Article : Google Scholar

16 

Wels J, Kaplan RN, Rafii S and Lyden D: Migratory neighbors and distant invaders: Tumor-associated niche cells. Genes Dev. 22:559–574. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Hoeben A, Landuyt B, Highley MS, Wildiers H, Van Oosterom AT and De Bruijn EA: Vascular endothelial growth factor and angiogenesis. Pharmacol Rev. 56:549–580. 2004. View Article : Google Scholar : PubMed/NCBI

18 

Neufeld G, Cohen T, Gengrinovitch S and Poltorak Z: Vascular endothelial growth factor (VEGF) and its receptors. FASEB J. 13:9–22. 1999.PubMed/NCBI

19 

Ferrara N: Vascular endothelial growth factor as a target for anticancer therapy. Oncologist. 9(Suppl 1): S2–S10. 2004. View Article : Google Scholar

20 

Ferrara N: VEGF as a therapeutic target in cancer. Oncology. 69(Suppl 3): S11–S16. 2005. View Article : Google Scholar

21 

Shibuya M: VEGF-VEGFR signals in health and disease. Biomol Ther (Seoul). 22:1–9. 2014. View Article : Google Scholar

22 

Ma J and Waxman DJ: Combination of antiangiogenesis with chemotherapy for more effective cancer treatment. Mol Cancer Ther. 7:3670–3684. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Miura S, Kondo J, Kawakami T, Shukunami C, Aimoto S, Tanaka H and Hiraki Y: Synthetic disulfide-bridged cyclic peptides mimic the anti-angiogenic actions of chondromodulin-I. Cancer Sci. 103:1311–1318. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Hakuno D and Fukuda K: Role of anti-angiogenic factor chondromodulin-I for maintaining cardiac valvular function. Clin Calcium. 17:361–372. 2007.In Japanese. PubMed/NCBI

25 

Klinger P, Surmann-Schmitt C, Brem M, Swoboda B, Distler JH, Carl HD, von der Mark K, Hennig FF and Gelse K: Chondromodulin 1 stabilizes the chondrocyte phenotype and inhibits endochondral ossification of porcine cartilage repair tissue. Arthritis Rheum. 63:2721–2731. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Hiraki Y, Inoue H, Iyama K, Kamizono A, Ochiai M, Shukunami C, Iijima S, Suzuki F and Kondo J: Identification of chondromodulin I as a novel endothelial cell growth inhibitor. Purification and its localization in the avascular zone of epiphyseal cartilage. J Biol Chem. 272:32419–32426. 1997. View Article : Google Scholar

27 

Fox SB, Generali DG and Harris AL: Breast tumour angiogenesis. Breast Cancer Res. 9:2162007. View Article : Google Scholar

28 

Miller KD: Recent translational research: Antiangiogenic therapy for breast cancer - where do we stand? Breast Cancer Res. 6:128–132. 2004. View Article : Google Scholar : PubMed/NCBI

29 

Muruve DA: The innate immune response to adenovirus vectors. Human Gene Ther. 15:1157–1166. 2004. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Shao J, Gan L and Wang J: Transfection of chondromodulin I into human breast cancer cells and its effect on the inhibition of cancer cell growth. Mol Med Rep 13: 4303-4308, 2016.
APA
Shao, J., Gan, L., & Wang, J. (2016). Transfection of chondromodulin I into human breast cancer cells and its effect on the inhibition of cancer cell growth. Molecular Medicine Reports, 13, 4303-4308. https://doi.org/10.3892/mmr.2016.5079
MLA
Shao, J., Gan, L., Wang, J."Transfection of chondromodulin I into human breast cancer cells and its effect on the inhibition of cancer cell growth". Molecular Medicine Reports 13.5 (2016): 4303-4308.
Chicago
Shao, J., Gan, L., Wang, J."Transfection of chondromodulin I into human breast cancer cells and its effect on the inhibition of cancer cell growth". Molecular Medicine Reports 13, no. 5 (2016): 4303-4308. https://doi.org/10.3892/mmr.2016.5079
Copy and paste a formatted citation
x
Spandidos Publications style
Shao J, Gan L and Wang J: Transfection of chondromodulin I into human breast cancer cells and its effect on the inhibition of cancer cell growth. Mol Med Rep 13: 4303-4308, 2016.
APA
Shao, J., Gan, L., & Wang, J. (2016). Transfection of chondromodulin I into human breast cancer cells and its effect on the inhibition of cancer cell growth. Molecular Medicine Reports, 13, 4303-4308. https://doi.org/10.3892/mmr.2016.5079
MLA
Shao, J., Gan, L., Wang, J."Transfection of chondromodulin I into human breast cancer cells and its effect on the inhibition of cancer cell growth". Molecular Medicine Reports 13.5 (2016): 4303-4308.
Chicago
Shao, J., Gan, L., Wang, J."Transfection of chondromodulin I into human breast cancer cells and its effect on the inhibition of cancer cell growth". Molecular Medicine Reports 13, no. 5 (2016): 4303-4308. https://doi.org/10.3892/mmr.2016.5079
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