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Metabolic profiling of Shu-Yu capsule in rat serum based on metabolic fingerprinting analysis using HPLC-ESI-MSn

  • Authors:
    • Fang Li
    • You‑Bo Zhang
    • Xia Wei
    • Chun‑Hong Song
    • Ming‑Qi Qiao
    • Hui‑Yun Zhang
  • View Affiliations / Copyright

    Affiliations: Hospital Pharmacy, Fengtai Maternal and Children's Health Hospital of Beijing, Beijing 100069, P.R. China, State Key Laboratory of Natural and Biomimetic Drugs and Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Peking University, Beijing 100101, P.R. China, Department of Pharmacology and Toxicology, Shandong Institute for Food and Drug Control, Jinan, Shandong 250012, P.R. China, Institute of Traditional Chinese Medicine Theory, School of Basic Medicine, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 100044, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4191-4204
    |
    Published online on: April 4, 2016
       https://doi.org/10.3892/mmr.2016.5082
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Abstract

The Chinese herbal formula, Shu-Yu capsule (SYC), has been successfully used to treat depression-like disorders in clinical settings. To rapidly identify the chemical constituents of SYC and its metabolites in rat serum, a simple and sensitive liquid chromatography-tandem mass spectrometry method was established in the present study. By comparing the retention times, MS and MSn spectra data in the literature and reference standards, a total of 73 compounds were identified from SYC. In rat serum, 62 components, including 13 prototype compounds and 49 metabolites were identified. Of these components, 14 metabolites were confirmed as novel metabolites of SYC. The results of the present study indicated that certain flavonoid glycosides and monoterpene glycosides were absorbed directly. Glucuronidation and sulfation were identified as the predominant metabolic pathways of the components in SYC. In addition, certain phase I reactions, including hydrolysis, demethylation and hydroxylation occurred in the rats. These results provide scientific evidence, which support further investigations of the pharmacology and mechanism of SYC.
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1 

Gao X, Sun P, Qiao M, Wei S, Xue L and Zhang H: Shu-Yu capsule, a traditional chinese medicine formulation, attenuates premenstrual syndrome depression induced by chronic stress constraint. Mol Med Rep. 10:2942–2948. 2014.PubMed/NCBI

2 

Chen SL and Chen DX: Clinical application of combined use of Radix Bupleuri and Radix Paeoniae Alba. Shanghai Journal of TCM. 41:71–74. 2007.In Chinese.

3 

Mao QQ, Xian YF, Ip SP, Tsai SH and Che CT: Long-term treatment with peony glycosides reverses chronic unpredictable mild stress-induced depressive-like behavior via increasing expression of neurotrophins in rat brain. Behav Brain Res. 210:171–177. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Kwon S, Lee B, Kim M, Lee H, Park HJ and Hahm DH: Antidepressant-like effect of the methanolic extract from Bupleurum falcatum in the tail suspension test. Prog Neuropsychopharmacol Biol Psychiatry. 34:265–270. 2010. View Article : Google Scholar

5 

Su GY, Yang JY, Wang F, Ma J, Zhang K, Dong YX, Song SJ, Lu XM and Wu CF: Antidepressant-like effects of xiaochaihutang in a rat model of chronic unpredictable mild stress. J Ethnopharmacol. 152:217–226. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Song CH, Li F, Guo YH and Zhang HY: Effects of Shuyu capsule and its main composition on protein expression and function of CACNA1C in hippocampus of rat with PMS liver-qi stagnation. Chinese Pharmacological Bulletin. 30:1476–1477. 2014.In Chinese.

7 

Wang XJ, Sun WJ, Sun H, Lv H, Wu Z, Wang P, Liu L and Cao H: Analysis of the constituents in the rat plasma after oral administration of Yin Chen Hao Tang by UPLC/Q-TOF-MS/MS. J Pharm Biomed Anal. 46:477–490. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Ge QF: Effects of ShuYu capsule on the expression of 5-HT3 receptor and its signal pathway of 5-HTR3-Ca2+-CaM in rat hippocampus neurons. Master's degree thesis. Shandong University of Traditional Chinese Medicine; pp. 32–33. 2012, In Chinese.

9 

National Research Council: Guide for the Care and Use of Laboratory Animals. Washington, DC: The National Academies Press; 1996, View Article : Google Scholar

10 

Li SL, Song JZ, Choi FF, Qiao CF, Zhou Y, Han QB and Xu HX: Chemical profiling of Radix Paeoniae evaluated by ultra-performance liquid chromatography/photo-diode-array/quadrupole time-of-flight mass spectrometry. J Pharm Biomed Anal. 49:253–266. 2009. View Article : Google Scholar

11 

Braca A, Kiem PV, Yen PH, Nhiem NX, Quang TH, Cuong NX and Minh CV: New monoterpene glycosides from Paeonia lactiflora. Fitoterapia. 79:117–120. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Lin HC, Ding HY, TW TS and Wu PL: Monoterpene glycosides from Paeonia suffruticosa. Phytochemistry. 41:237–242. 1996. View Article : Google Scholar

13 

Su J, Zhang P, Zhang JJ, Qi XM, Wu YG and Shen JJ: Effects of total glucosides of paeony on oxidative stress in the kidney from diabetic rats. Phytomedicine. 17:254–260. 2010. View Article : Google Scholar

14 

Liang J, Xu F, Zhang YZ, Huang S, Zang XY, Zhao X, Zhang L, Shang MY, Yang DH, Wang X and Cai SQ: The profiling and identification of the absorbed constituents and metabolites of Paeoniae Radix Rubra decoction in rat plasma and urine by the HPLC-DAD-ESI-IT-TOF-MS(n) technique: A novel strategy for the systematic screening and identification of absorbed constituents and metabolites from traditional Chinese medicines. J Pharm Biomed Anal. 83:108–121. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Xiao S, Luo K, Wen X, Fan X and Cheng Y: A pre-classification strategy for identification of compounds in traditional Chinese medicine analogous formulas by high-performance liquid chromatography-Mass spectrometry. J Pharm Biomed Anal. 92:82–89. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Yang YY, Tang YZ, Fan CL, Luo HT, Guo PR and Chen JX: Identification and determination of the saikosaponins in Radix bupleuri by accelerated solvent extraction combined with rapid-resolution LC-MS. J Sep Sci. 33:1933–1945. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Chen L, Qi J, Chang YX, Zhu D and Yu B: Identification and determination of the major constituents in Traditional Chinese Medicinal formula Danggui-Shaoyao-San by HPLC-DAD-ESI-MS/MS. J Pharm Biomed Anal. 50:127–137. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Wang DD, Liang J, Yang WZ, Hou JJ, Yang M, Da J, Wang Y, Jiang BH, Liu X, Wu WY and Guo DA: HPLC/q TOF-MS-oriented characteristic components data set and chemometric analysis for the holistic quality control of complex TCM preparations: Niuhuang Shangqing pill as an example. J Pharm Biomed Anal. 89:130–141. 2014. View Article : Google Scholar

19 

Bao Y, Li C, Shen H and Nan F: Determination of saikosaponin derivatives in Radix bupleuri and in pharmaceuticals of the chinese multiherb remedy xiaochaihutang using liquid chromatographic tandem mass spectrometry. Anal Chem. 76:4208–4216. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Liang Z, Oh K, Wang Y, Yi T, Chen H and Zhao Z: Cell type-specific qualitative and quantitative analysis of saikosaponins in three Bupleurum species using laser microdissection and liquid chromatography-quadrupole/time of flight-mass spectrometry. J Pharm Biomed Anal. 97:157–165. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Yan Y, Chai CZ, Wang DW, Yue XY, Zhu DN and Yu BY: HPLC-DAD-Q-TOF-MS/MS analysis and HPLC quantitation of chemical constituents in traditional Chinese medicinal formula Ge-Gen decoction. J Pharm Biomed Anal. 80:192–202. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Wang S, Chen L, Leng J, Chen P, Fan X and Cheng Y: Fragment ion diagnostic strategies for the comprehensive identification of chemical profile of Gui-Zhi-Tang by integrating high-resolution MS, multiple-stage MS and UV information. J Pharm Biomed Anal. 98:22–35. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Yan Z, Chen Y, Li T, Zhang J and Yang X: Identification of metabolites of Si-Ni-San, a traditional Chinese medicine formula, in rat plasma and urine using liquid chromatography/diode array detection/triple-quadrupole spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 885–886:73–82. 2012. View Article : Google Scholar

24 

Wang Y, He S, Cheng X, Lu Y, Zou Y and Zhang Q: UPLC-Q-TOF-MS/MS fingerprinting of traditional Chinese formula SiJunZiTang. J Pharm Biomed Anal. 80:24–33. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Xiang C, Qiao X, Ye M and Guo DA: Classification and distribution analysis of compounds in Glycyrrhiza using licorice compounds database. Yao Xue Xue Bao. 47:1023–1030. 2012.In Chinese. PubMed/NCBI

26 

Zhang W, Saif MW, Dutschman GE, Li X, Lam W, Bussom S, Jiang Z, Ye M, Chu E and Cheng YC: Identification of chemicals and their metabolites from PHY906, a Chinese medicine formulation, in the plasma of a patient treated with irinotecan and PHY906 using liquid chromatography/tandem mass spectrometry (LC/MS/MS). J Chromatogr A. 1217:5785–5793. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Holcapek M, Kolárová L and Nobilis M: High-performance liquid chromatography-tandem mass spectrometry in the identification and determination of phase I and phase II drug metabolites. Anal Bioanal Chem. 391:59–78. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Lu LL, Shu Y, Qian DW, Su SL, Duan JA, Qian YF and Xue CF: Identification of the metabolites of Sinisan extract in rat plasma, urine, feces and bile after intragastric administration. Acta Pharm Sin. 46:1374–1379. 2011.In Chinese.

29 

Tolleson WH, Doerge DR, Churchwell MI, Marques MM and Roberts DW: Metabolism of biochanin A and formononetin by human liver microsomes in vitro. J Agric Food Chem. 50:4783–4790. 2002. View Article : Google Scholar : PubMed/NCBI

30 

Su ZH, Zou GA, Preiss A, Zhang HW and Zou ZM: Online identification of the antioxidant constituents of traditional Chinese medicine formula Chaihu-Shu-Gan-San by LC-LTQ-Orbitrap mass spectrometry and microplate spectrophotometer. J Pharm Biomed Anal. 53:454–461. 2010. View Article : Google Scholar : PubMed/NCBI

31 

Qiao X, Ye M, Xiang C, Wang Q, Liu CF, Miao WJ and Guo DA: Analytical strategy to reveal the in vivo process of multi-component herbal medicine: A pharmacokinetic study of licorice using liquid chromatography coupled with triple quadrupole mass spectrometry. J Chromatogr A. 1258:84–93. 2012. View Article : Google Scholar : PubMed/NCBI

32 

Huang HQ, Zhang X, Xu ZX, Su J, Yan SK and Zhang WD: Fast determination of saikosaponins in Bupleurum by rapid resolution liquid chromatography with evaporative light scattering detection. J Pharm Biomed Anal. 49:1048–1055. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li F, Zhang YB, Wei X, Song CH, Qiao MQ and Zhang HY: Metabolic profiling of Shu-Yu capsule in rat serum based on metabolic fingerprinting analysis using HPLC-ESI-MSn. Mol Med Rep 13: 4191-4204, 2016.
APA
Li, F., Zhang, Y., Wei, X., Song, C., Qiao, M., & Zhang, H. (2016). Metabolic profiling of Shu-Yu capsule in rat serum based on metabolic fingerprinting analysis using HPLC-ESI-MSn. Molecular Medicine Reports, 13, 4191-4204. https://doi.org/10.3892/mmr.2016.5082
MLA
Li, F., Zhang, Y., Wei, X., Song, C., Qiao, M., Zhang, H."Metabolic profiling of Shu-Yu capsule in rat serum based on metabolic fingerprinting analysis using HPLC-ESI-MSn". Molecular Medicine Reports 13.5 (2016): 4191-4204.
Chicago
Li, F., Zhang, Y., Wei, X., Song, C., Qiao, M., Zhang, H."Metabolic profiling of Shu-Yu capsule in rat serum based on metabolic fingerprinting analysis using HPLC-ESI-MSn". Molecular Medicine Reports 13, no. 5 (2016): 4191-4204. https://doi.org/10.3892/mmr.2016.5082
Copy and paste a formatted citation
x
Spandidos Publications style
Li F, Zhang YB, Wei X, Song CH, Qiao MQ and Zhang HY: Metabolic profiling of Shu-Yu capsule in rat serum based on metabolic fingerprinting analysis using HPLC-ESI-MSn. Mol Med Rep 13: 4191-4204, 2016.
APA
Li, F., Zhang, Y., Wei, X., Song, C., Qiao, M., & Zhang, H. (2016). Metabolic profiling of Shu-Yu capsule in rat serum based on metabolic fingerprinting analysis using HPLC-ESI-MSn. Molecular Medicine Reports, 13, 4191-4204. https://doi.org/10.3892/mmr.2016.5082
MLA
Li, F., Zhang, Y., Wei, X., Song, C., Qiao, M., Zhang, H."Metabolic profiling of Shu-Yu capsule in rat serum based on metabolic fingerprinting analysis using HPLC-ESI-MSn". Molecular Medicine Reports 13.5 (2016): 4191-4204.
Chicago
Li, F., Zhang, Y., Wei, X., Song, C., Qiao, M., Zhang, H."Metabolic profiling of Shu-Yu capsule in rat serum based on metabolic fingerprinting analysis using HPLC-ESI-MSn". Molecular Medicine Reports 13, no. 5 (2016): 4191-4204. https://doi.org/10.3892/mmr.2016.5082
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