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Article

Inhibition of the Rac1-WAVE2-Arp2/3 signaling pathway promotes radiosensitivity via downregulation of cofilin-1 in U251 human glioma cells

  • Authors:
    • Tao Zhou
    • Chen‑Han Wang
    • Hua Yan
    • Rui Zhang
    • Jin‑Bing Zhao
    • Chun‑Fa Qian
    • Hong Xiao
    • Hong‑Yi Liu
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Nanjing Medical University, Affiliated Nanjing Brain Hospital, Nanjing, Jiangsu 210029, P.R. China, Neuropsychiatric Institute, Nanjing Medical University, Affiliated Nanjing Brain Hospital, Nanjing, Jiangsu 210029, P.R. China
  • Pages: 4414-4420
    |
    Published online on: April 5, 2016
       https://doi.org/10.3892/mmr.2016.5088
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Abstract

The Ras-related C3 botulinum toxin substrate 1 (Rac1)-WASP-family verprolin-homologous protein-2 (WAVE2)-actin-related protein 2/3 (Arp2/3) signaling pathway has been identified to be involved in cell migration and invasion in various types of cancer cell. Cofilin‑1 (CFL‑1), which is regulated by the Rac1‑WAVE2‑Arp2/3 signaling pathway, may promote radioresistance in glioma. Therefore, the present study aimed to investigate the potential role of the Rac1‑WAVE2‑Arp2/3 signaling pathway in radioresistance in U251 human glioma cells and elucidate its affect on CFL‑1 expression. Western blot analysis was performed to evaluate the protein expression of CFL‑1. In the present study, Rac1 was inhibited by NSC 23766, WAVE2 was inhibited by transfection with short hairpin (sh)RNA‑WAVE2 using Lipofectamine™ 2000 and Arp2/3 was inhibited by CK‑666. Cell viability was measured using the 3‑(4,5‑dimethylthiazol‑2‑yl)-2,5‑diphenyltetrazolium bromide assay, the cell migration ability was examined by a wound‑healing assay, and the cell invasion ability was assessed using a Transwell culture chamber system. The results showed that inhibition of the Rac1‑WAVE2‑Arp2/3 signaling pathway using NSC 23766, shRNA‑WAVE2 or CK‑666 reduced the cell viability, migration and invasion abilities in U251 human glioma cells, concordant with a reduced expression of CFL‑1. Furthermore, the expression of CFL‑1 was significantly increased in radioresistant U251 glioma cells when compared with normal U251 human glioma cells. These findings indicate that inhibition of the Rac1‑WAVE2‑Arp2/3 signaling pathway may promote radiosensitivity, which may partially result from the downregulation of CFL‑1 in U251 human glioma cells.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou T, Wang CH, Yan H, Zhang R, Zhao JB, Qian CF, Xiao H and Liu HY: Inhibition of the Rac1-WAVE2-Arp2/3 signaling pathway promotes radiosensitivity via downregulation of cofilin-1 in U251 human glioma cells. Mol Med Rep 13: 4414-4420, 2016.
APA
Zhou, T., Wang, C., Yan, H., Zhang, R., Zhao, J., Qian, C. ... Liu, H. (2016). Inhibition of the Rac1-WAVE2-Arp2/3 signaling pathway promotes radiosensitivity via downregulation of cofilin-1 in U251 human glioma cells. Molecular Medicine Reports, 13, 4414-4420. https://doi.org/10.3892/mmr.2016.5088
MLA
Zhou, T., Wang, C., Yan, H., Zhang, R., Zhao, J., Qian, C., Xiao, H., Liu, H."Inhibition of the Rac1-WAVE2-Arp2/3 signaling pathway promotes radiosensitivity via downregulation of cofilin-1 in U251 human glioma cells". Molecular Medicine Reports 13.5 (2016): 4414-4420.
Chicago
Zhou, T., Wang, C., Yan, H., Zhang, R., Zhao, J., Qian, C., Xiao, H., Liu, H."Inhibition of the Rac1-WAVE2-Arp2/3 signaling pathway promotes radiosensitivity via downregulation of cofilin-1 in U251 human glioma cells". Molecular Medicine Reports 13, no. 5 (2016): 4414-4420. https://doi.org/10.3892/mmr.2016.5088
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou T, Wang CH, Yan H, Zhang R, Zhao JB, Qian CF, Xiao H and Liu HY: Inhibition of the Rac1-WAVE2-Arp2/3 signaling pathway promotes radiosensitivity via downregulation of cofilin-1 in U251 human glioma cells. Mol Med Rep 13: 4414-4420, 2016.
APA
Zhou, T., Wang, C., Yan, H., Zhang, R., Zhao, J., Qian, C. ... Liu, H. (2016). Inhibition of the Rac1-WAVE2-Arp2/3 signaling pathway promotes radiosensitivity via downregulation of cofilin-1 in U251 human glioma cells. Molecular Medicine Reports, 13, 4414-4420. https://doi.org/10.3892/mmr.2016.5088
MLA
Zhou, T., Wang, C., Yan, H., Zhang, R., Zhao, J., Qian, C., Xiao, H., Liu, H."Inhibition of the Rac1-WAVE2-Arp2/3 signaling pathway promotes radiosensitivity via downregulation of cofilin-1 in U251 human glioma cells". Molecular Medicine Reports 13.5 (2016): 4414-4420.
Chicago
Zhou, T., Wang, C., Yan, H., Zhang, R., Zhao, J., Qian, C., Xiao, H., Liu, H."Inhibition of the Rac1-WAVE2-Arp2/3 signaling pathway promotes radiosensitivity via downregulation of cofilin-1 in U251 human glioma cells". Molecular Medicine Reports 13, no. 5 (2016): 4414-4420. https://doi.org/10.3892/mmr.2016.5088
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