Digital gene expression profiling analysis of childhood acute lymphoblastic leukemia

  • Authors:
    • Ming‑Yi Zhao
    • Yan Yu
    • Min Xie
    • Ming‑Hua Yang
    • Shan Zhu
    • Liang‑Chun Yang
    • Rui Kang
    • Dao‑Lin Tang
    • Ling‑Ling Zhao
    • Li‑Zhi Cao
  • View Affiliations

  • Published online on: April 5, 2016     https://doi.org/10.3892/mmr.2016.5089
  • Pages: 4321-4328
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Abstract

Acute lymphoblastic leukemia (ALL) is the most commonly diagnosed malignancy in children. It is a heterogeneous disease, and is determined by multiple gene alterations and chromosomal rearrangements. To improve current understanding of the underlying molecular mechanisms of ALL, the present study profiled genome‑wide digital gene expression (DGE) in a population of children with ALL in China. Using second‑generation sequencing technology, the profiling revealed that 2,825 genes were upregulated and 1,952 were downregulated in the ALL group. Based on the DGE profiling data, the present study further investigated seven genes (WT1, RPS26, MSX1, CD70, HOXC4, HOXA5 and HOXC6) using reverse transcription‑quantitative polymerase chain reaction analysis. Gene Ontology analysis suggested that the differentially expressed genes were predominantly involved in immune cell differentiation, metabolic processes and programmed cell death. The results of the present study provided novel insights into the gene expression patterns in children with ALL.
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May-2016
Volume 13 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Zhao MY, Yu Y, Xie M, Yang MH, Zhu S, Yang LC, Kang R, Tang DL, Zhao LL, Cao LZ, Cao LZ, et al: Digital gene expression profiling analysis of childhood acute lymphoblastic leukemia. Mol Med Rep 13: 4321-4328, 2016
APA
Zhao, M., Yu, Y., Xie, M., Yang, M., Zhu, S., Yang, L. ... Cao, L. (2016). Digital gene expression profiling analysis of childhood acute lymphoblastic leukemia. Molecular Medicine Reports, 13, 4321-4328. https://doi.org/10.3892/mmr.2016.5089
MLA
Zhao, M., Yu, Y., Xie, M., Yang, M., Zhu, S., Yang, L., Kang, R., Tang, D., Zhao, L., Cao, L."Digital gene expression profiling analysis of childhood acute lymphoblastic leukemia". Molecular Medicine Reports 13.5 (2016): 4321-4328.
Chicago
Zhao, M., Yu, Y., Xie, M., Yang, M., Zhu, S., Yang, L., Kang, R., Tang, D., Zhao, L., Cao, L."Digital gene expression profiling analysis of childhood acute lymphoblastic leukemia". Molecular Medicine Reports 13, no. 5 (2016): 4321-4328. https://doi.org/10.3892/mmr.2016.5089