Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
June-2016 Volume 13 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June-2016 Volume 13 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Treatment of experimental autoimmune uveoretinitis with different natural compounds

  • Authors:
    • Man Li
    • Xiaoming Chen
    • Juanjuan Liu
    • Dongmei Wang
    • Lu Gan
    • Xin Lv
    • Yu Qiao
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, P.R. China, Department of Ophthalmology, AVIC 363 Hospital of Chengdu, Chengdu, Sichuan 610041, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4654-4658
    |
    Published online on: April 8, 2016
       https://doi.org/10.3892/mmr.2016.5096
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Uveitis is an important eye disease that potentially causes loss of sight. Although extensive studies have been conducted on uveitis, the exact pathogenesis remains to be determined. The effects of treatment with natural compounds on an experimental autoimmune uveoretinitis (EAU) rat model were examined in the present study. A total of 25 rats were divided into 5 groups: Alkaloids (n=5), saponins (n=5), flavonoids (n=5), phenols (n=5), and the normal saline group (n=5). The rats in each group were treated with an intraperitoneal injection of proper alkaloids (berberine hydrochloride), saponins (steroidal saponins), flavonoids (baicalein), or phenols (chlorogenic acid) or physiological saline, respectively. The rats' aqueous humour and crystalline lens was then observed under the slit lamp periodically, looking for signs of inflammation. After 2 weeks, the rats were sacrificed and the degree of pathological changes on their eyeballs under different treatment methods were determined using an optical microscope. The expression of the interleukin (IL)‑17 gene in the ocular tissues of the rats was assessed via RT‑PCR and western blot analysis. Apoptosis on the rats' retinal tissues was detected using flow cytometry. The results showed that rats injected with phenols (chlorogenic acid) had serious ocular vascular dilatation, iris hemorrhage and purulent exudation; those injected with alkaloids (berberine hydrochloride) and flavonoids (baicalein) had a more mild form of inflammation; and those administered saponins (steroidal saponins) had only mild inflammation signs. Following detection of IL‑17 mRNA and protein expression levels in the ocular tissues of rats of the five groups, it was found that their expression was lowest in the saponin‑treated group and the other differences in expression were all statistically significant (P<0.05). A comparison with other groups revealed that cell apoptosis in the eyes of rats in the saponin group was the most prominent, reflecting a beneficial decrease in the amount of inflammatory cells on the lesion. Based on these findings, natural compounds such as saponins (steroidal saponins), alkaloids (berberine hydrochloride), and flavonoids (baicalein), but not phenols (chlorogenic acid), can inhibit the clinical symptoms of EAU in rats to a certain extent and reduce cell apoptosis. The most promising results in the present study were obtained using steroidal saponins.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Witkowski L, Cywinska A, Paschalis-Trela K, Crisman M and Kita J: Multiple etiologies of equine recurrent uveitis - A natural model for human autoimmune uveitis: A brief review. Comp Immunol Microbiol Infect Dis. 44:14–20. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Wakefield D and Abi-Hanna D: HLA antigens and their significance in the pathogenesis of anterior uveitis: A mini review. Curr Eye Res. 5:465–473. 1986. View Article : Google Scholar : PubMed/NCBI

3 

Maca SM, Amirian A, Prause C, Gruber K, Mejdoubi L and Barisani-Asenbauer T: Understanding the impact of uveitis on health-related quality of life in adolescents. Acta Ophthalmol. 91:e219–e224. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Berthet F, Le Deist F, Duliege AM, Griscelli C and Fischer A: Clinical consequences and treatment of primary immunodeficiency syndromes characterized by functional T and B lymphocyte anomalies (combined immune deficiency). Pediatrics. 93:265–270. 1994.PubMed/NCBI

5 

Zhang Q, Lu J, Liang X and Zhi L: The level and clinical significance of serum IL-17 in patients with systemic lupus erythematosus. Xibao Yu Fenzi Mianyixue Zazhi. 28:301–303. 2012.In Chinese.

6 

Cui Y, Wang G, Li Y, Wang Y, Wang X and Bi H: Optical coherence tomography and histopathology of macular uveitis. Optom Vis Sci. 91:1335–1342. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Oh-i K, Keino H, Goto H, Yamakawa N, Murase K, Usui Y, Kezuka T, Sakai J, Takeuchi M and Usui M: Intravitreal injection of Tacrolimus (FK506) suppresses ongoing experimental autoimmune uveoretinitis in Rats. Br J Ophthalmol. 91:237–242. 2007. View Article : Google Scholar

8 

Kohno H, Sakai T, Saito S, Okano K and Kitahara K: Treatment of experimental autoimmune uveoretinitis with atorvastatin and lovastatin. Exp Eye Res. 84:569–576. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Kezic JM, Glant TT, Rosenbaum JT and Rosenzweig HL: Neutralization of IL-17 ameliorates uveitis but damages photo-receptors in a murine model of spondyloarthritis. Arthritis Res Ther. 14:R182012. View Article : Google Scholar

10 

Chi W, Zhu X, Yang P, Liu X, Lin X, Zhou H, Huang X and Kijlstra A: Upregulated IL-23 and IL-17 in Behçet patients with active uveitis. Invest Ophthalmol Vis Sci. 49:3058–3064. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Sng CC, Ang M and Barton K: Uveitis and glaucoma: New insights in the pathogenesis and treatment. Prog Brain Res. 221:243–269. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Wells JM and Smith JR: Uveitis in juvenile idiopathic arthritis: Recent therapeutic advances. Ophthalmic Res. 54:124–127. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Zou ZP, Qiang FY, Zhang T, Sun L, Jia T, Zhu XC and Xu H: Treatment of experimental autoimmune uveitis in rats with arsenic trioxide. Chin Ophthalmic Res. 28:306–310. 2010.

14 

Klímová A, Seidler Štanogová P, Heissigerová J, Svozílková P and Kučera T: Mycophenolate mofetil and cyclophosphamide treatments suppress inflammation intensity in an experimental model of autoimmune uveitis. Folia Biol (Praha). 60:228–234. 2014.

15 

Tang Y and Cai L: Research progress of regulatory T cells and uveitis. Int J Ophthalmol. 11:450–452. 2011.

16 

Li J, Chen X, Liu Z, Yue Q and Liu H: Expression of Th17 cytokines in skin lesions of patients with psoriasis. J Huazhong Univ Sci Technolog Med Sci. 27:330–332. 2007. View Article : Google Scholar : PubMed/NCBI

17 

de Smet MD, Taylor SRJ, Bodaghi B, Miserocchi E, Murray PI, Pleyer U, Zierhut M, Barisani-Asenbauer T, LeHoang P and Lightman S: Understanding uveitis: The impact of research on visual outcomes. Prog Retin Eye Res. 30:452–470. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Mira A, Tanaka A, Tateyama Y, Kondo R and Shimizu K: Comparative biological study of roots, stems, leaves, and seeds of Angelica shikokiana Makino. J Ethnopharmacol. 148:980–987. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Byun MW: Schizonepeta tenuifolia ethanol extract exerts anti-inflammatory activity through the inhibition of TLR4 signaling in lipopolysaccharide-stimulated macrophage cells. J Med Food. 17:350–356. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Jäger A and Kuchroo VK: Effector and regulatory T-cell subsets in autoimmunity and tissue inflammation. Scand J Immunol. 72:173–184. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Tang K, Si J-K, Guo D-D, Cui Y, Du YX, Pan XM and Bi HS: Ranibizumab alone or in combination with photodynamic therapy vs photodynamic therapy for polypoidal choroidal vasculopathy: A systematic review and Meta-analysis. Int J Ophthalmol. 8:1056–1066. 2015.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li M, Chen X, Liu J, Wang D, Gan L, Lv X and Qiao Y: Treatment of experimental autoimmune uveoretinitis with different natural compounds. Mol Med Rep 13: 4654-4658, 2016.
APA
Li, M., Chen, X., Liu, J., Wang, D., Gan, L., Lv, X., & Qiao, Y. (2016). Treatment of experimental autoimmune uveoretinitis with different natural compounds. Molecular Medicine Reports, 13, 4654-4658. https://doi.org/10.3892/mmr.2016.5096
MLA
Li, M., Chen, X., Liu, J., Wang, D., Gan, L., Lv, X., Qiao, Y."Treatment of experimental autoimmune uveoretinitis with different natural compounds". Molecular Medicine Reports 13.6 (2016): 4654-4658.
Chicago
Li, M., Chen, X., Liu, J., Wang, D., Gan, L., Lv, X., Qiao, Y."Treatment of experimental autoimmune uveoretinitis with different natural compounds". Molecular Medicine Reports 13, no. 6 (2016): 4654-4658. https://doi.org/10.3892/mmr.2016.5096
Copy and paste a formatted citation
x
Spandidos Publications style
Li M, Chen X, Liu J, Wang D, Gan L, Lv X and Qiao Y: Treatment of experimental autoimmune uveoretinitis with different natural compounds. Mol Med Rep 13: 4654-4658, 2016.
APA
Li, M., Chen, X., Liu, J., Wang, D., Gan, L., Lv, X., & Qiao, Y. (2016). Treatment of experimental autoimmune uveoretinitis with different natural compounds. Molecular Medicine Reports, 13, 4654-4658. https://doi.org/10.3892/mmr.2016.5096
MLA
Li, M., Chen, X., Liu, J., Wang, D., Gan, L., Lv, X., Qiao, Y."Treatment of experimental autoimmune uveoretinitis with different natural compounds". Molecular Medicine Reports 13.6 (2016): 4654-4658.
Chicago
Li, M., Chen, X., Liu, J., Wang, D., Gan, L., Lv, X., Qiao, Y."Treatment of experimental autoimmune uveoretinitis with different natural compounds". Molecular Medicine Reports 13, no. 6 (2016): 4654-4658. https://doi.org/10.3892/mmr.2016.5096
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team