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Circulating microRNAs as biomarkers for the early diagnosis of childhood tuberculosis infection

  • Authors:
    • Mengyao Zhou
    • Guangyuan Yu
    • Xiantao Yang
    • Chaomin Zhu
    • Zhenzhen Zhang
    • Xue Zhan
  • View Affiliations / Copyright

    Affiliations: The Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing 400000, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4620-4626
    |
    Published online on: April 8, 2016
       https://doi.org/10.3892/mmr.2016.5097
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Abstract

MicroRNAs (miRNAs) are a class of highly conserved, single-stranded RNA molecules (length, 18-25 nt) that regulate the expression of their target mRNAs. Previous studies have demonstrated that miRNAs may be novel biomarkers in the diagnosis of certain diseases. In order to evaluate the diagnostic value of miRNAs in childhood tuberculosis (TB), the circulating miRNA profile was determined using microarray analysis. An miRNA‑gene network was constructed to identify closely associated miRNAs and these miRNAs were validated using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). A receiver operational curve (ROC) was used to evaluate the diagnostic sensitivity and specificity of confirmed miRNAs. The microarray data demonstrated that 29 miRNAs were altered with 15 upregulated and 14 downregulated. The network showed indicated 14 miRNAs that are critical in childhood TB. RT-qPCR validated that miR-1, miR-155, miR‑31, miR‑146a, miR‑10a, miR‑125b and miR‑150 were downregulated in while miR‑29 was upregulated in children with TB compared with uninfected children. The ROC curve data indicated the diagnostic value of single miRNA was as follows: miR‑150>miR‑146a>miR‑125b>miR‑31>miR‑10a>miR‑1>miR‑155>miR‑29. Notably, a combination of these miRNAs exhibited increased diagnostic value compared with any single miRNA. To the best of our knowledge, the present study is the first to identify the expression profile of circulating miRNAs in childhood TB and demonstrated that miRNAs may be a novel, non‑invasive and effective biomarker for the early diagnosis of childhood TB.
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View References

1 

World Health Organization: Global Tuberculosis Report 2015. 20th edition. World Health Organization; 2015

2 

Li L: The national epidemiological sampling survey analysis of childhood tuberculosis from 1979 to 2000. Chinese Medical Journal. 80:16782004.In Chinese.

3 

World Health Organisation: Global tuberculosis report 2013. 2013

4 

Krishnarao A: MicroRNAs – From Basic Science to Disease Biology. Cambridge University Press; Cambridge, UK: 2010

5 

He L and Hannon GJ: MicroRNAs: Small RNAs with a big role in gene regulation. Nat Rev Genet. 5:522–531. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Chim SS, Shing TK, Hung EC, Leung TY, Lau TK, Chiu RW and Lo YM: Detection and characterization of placental microRNAs in maternal plasma. Clin Chem. 54:482–490. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Alevizos I and Illei GG: MicroRNAs as biomarkers in rheumatic diseases. Nat Rev Rheumatol. 6:391–398. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Gilad S, Meiri E, Yogev Y, Benjamin S, Lebanony D, Yerushalmi N, Benjamin H, Kushnir M, Cholakh H, Melamed N, et al: Serum microRNAs are promising novel biomarkers. PloS One. 3:e31482008. View Article : Google Scholar : PubMed/NCBI

9 

Chen X, Hu Z, Wang W, Ba Y, Ma L, Zhang C, Wang C, Ren Z, Zhao Y, Wu S, et al: Identification of ten serum microRNAs from a genome-wide serum microRNA expression profile as novel noninvasive biomarkers for nonsmall cell lung cancer diagnosis. Int J Cancer. 130:1620–1628. 2012. View Article : Google Scholar

10 

Kosaka N, Iguchi H and Ochiya T: Circulating microRNA in body fluid: A new potential biomarker for cancer diagnosis and prognosis. Cancer Sci. 101:2087–2092. 2010. View Article : Google Scholar : PubMed/NCBI

11 

O'Connell RM, Kahn D, Gibson WS, Round JL, Scholz RL, Chaudhuri AA, Kahn ME, Rao DS and Baltimore D: MicroRNA-155 promotes autoimmune inflammation by enhancing inflammatory T cell development. Immunity. 33:607–619. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Ura S, Honda M, Yamashita T, Ueda T, Takatori H, Nishino R, Sunakozaka H, Sakai Y, Horimoto K and Kaneko S: Differential microRNA expression between hepatitis B and hepatitis C leading disease progression to hepatocellular carcinoma. Hepatology. 49:1098–1112. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Gidlöf O and Erlinge D: MicroRNAs in the failing heart-novel therapeutic targets? Scand Cardiovasc J. 48:328–334. 2014. View Article : Google Scholar

14 

Ji J, Shi J, Budhu A, Yu Z, Forgues M, Roessler S, Ambs S, Chen Y, Meltzer PS, Croce CM, et al: MicroRNA expression, survival and response to interferon in liver cancer. N Engl J Med. 361:1437–1447. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Fu Y, Yi Z, Wu X, Li J and Xu F: Circulating microRNAs in patients with active pulmonary tuberculosis. J Clin Microbiol. 49:4246–4251. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Spinelli SV, Diaz A, D'Attilio L, Marchesini MM, Bogue C, Bay ML and Bottasso OA: Altered microRNA expression levels in mononuclear cells of patients with pulmonary and pleural tuberculosis and their relation with components of the immune response. Mol Immunol. 53:265–269. 2013. View Article : Google Scholar

17 

Zhang X, Guo J, Fan S, Li Y, Wei L, Yang X, Jiang T, Chen Z, Wang C, Liu J, et al: Screening and identification of six serum microRNAs as novel potential combination biomarkers for pulmonary tuberculosis diagnosis. PloS One. 8:e810762013. View Article : Google Scholar : PubMed/NCBI

18 

Abd-El-Fattah AA, Sadik NA, Shaker OG and Aboulftouh ML: Differential microRNAs expression in serum of patients with lung cancer, pulmonary tuberculosis and pneumonia. Cell Biochem Biophys. 67:875–884. 2013. View Article : Google Scholar

19 

Subspecialty Group of Respiratory Diseases; Society of Pediatrics, Chinese Medical Association: Editorial Board, Chinese Journal of Pediatrics: Diagnostic standards and therapeutic recommendations for pulmonary tuberculosis in children. Zhonghua Er Ke Za Zhi. 44:249–251. 2006.In Chinese.

20 

Schmittgen TD and Livak KJ: Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 3:1101–1108. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Anderson ST, Kaforou M, Brent AJ, Wright VJ, Banwell CM, Chagaluka G, Crampin AC, Dockrell HM, French N, Hamilton MS, et al: Diagnosis of childhood tuberculosis and host RNA expression in Africa. N Engl J Med. 370:1712–1723. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Eamranond P and Jaramillo E: Tuberculosis in children: Reassessing the need for improved diagnosis in global control strategies. Int J Tuberc Lung Dis. 5:594–603. 2001.PubMed/NCBI

23 

Zar HJ, Connell TG and Nicol M: Diagnosis of pulmonary tuberculosis in children: New advances. Expert Rev Anti Infect Ther. 8:277–288. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Wallis RS, Pai M, Menzies D, Doherty TM, Walzl G, Perkins MD and Zumla A: Biomarkers and diagnostics for tuberculosis: Progress, needs and translation into practice. Lancet. 375:1920–1937. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Maertzdorf J, Weiner J III, Mollenkopf HJ; TBornotTB Network; Bauer T, Prasse A, Müller-Quernheim J and Kaufmann SH: Common patterns and disease-related signatures in tuberculosis and sarcoidosis. Proc Natl Acad Sci USA. 109:7853–7858. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Qi Y, Cui L, Ge Y, Shi Z, Zhao K, Guo X, Yang D, Yu H, Cui L, Shan Y, et al: Altered serum microRNAs as biomarkers for the early diagnosis of pulmonary tuberculosis infection. BMC Infect Dis. 12:384–393. 2012. View Article : Google Scholar

27 

Wang C, Yang S, Sun G, Tang X, Lu S, Neyrolles O and Gao Q: Comparative miRNA expression profiles in individuals with latent and active tuberculosis. PloS One. 6:e258322011. View Article : Google Scholar : PubMed/NCBI

28 

Yi Z, Fu Y, Ji R, Li R and Guan Z: Altered microRNA signatures in sputum of patients with active pulmonary tuberculosis. PloS One. 7:e431842012. View Article : Google Scholar : PubMed/NCBI

29 

Liu Y, Wang X, Jiang J, Cao Z, Yang B and Cheng X: Modulation of T cell cytokine production by miR-144* with elevated expression in patients with pulmonary tuberculosis. Mol Immunol. 48:1084–1090. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Wu J, Lu C, Diao N, Zhang S, Wang S, Wang F, Gao Y, Chen J, Shao L, Lu J, et al: Analysis of microRNA expression profiling identifies miR-155 and miR-155* as potential diagnostic markers for active tuberculosis: A preliminary study. Hum Immunol. 73:31–37. 2012. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Zhou M, Yu G, Yang X, Zhu C, Zhang Z and Zhan X: Circulating microRNAs as biomarkers for the early diagnosis of childhood tuberculosis infection. Mol Med Rep 13: 4620-4626, 2016.
APA
Zhou, M., Yu, G., Yang, X., Zhu, C., Zhang, Z., & Zhan, X. (2016). Circulating microRNAs as biomarkers for the early diagnosis of childhood tuberculosis infection. Molecular Medicine Reports, 13, 4620-4626. https://doi.org/10.3892/mmr.2016.5097
MLA
Zhou, M., Yu, G., Yang, X., Zhu, C., Zhang, Z., Zhan, X."Circulating microRNAs as biomarkers for the early diagnosis of childhood tuberculosis infection". Molecular Medicine Reports 13.6 (2016): 4620-4626.
Chicago
Zhou, M., Yu, G., Yang, X., Zhu, C., Zhang, Z., Zhan, X."Circulating microRNAs as biomarkers for the early diagnosis of childhood tuberculosis infection". Molecular Medicine Reports 13, no. 6 (2016): 4620-4626. https://doi.org/10.3892/mmr.2016.5097
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou M, Yu G, Yang X, Zhu C, Zhang Z and Zhan X: Circulating microRNAs as biomarkers for the early diagnosis of childhood tuberculosis infection. Mol Med Rep 13: 4620-4626, 2016.
APA
Zhou, M., Yu, G., Yang, X., Zhu, C., Zhang, Z., & Zhan, X. (2016). Circulating microRNAs as biomarkers for the early diagnosis of childhood tuberculosis infection. Molecular Medicine Reports, 13, 4620-4626. https://doi.org/10.3892/mmr.2016.5097
MLA
Zhou, M., Yu, G., Yang, X., Zhu, C., Zhang, Z., Zhan, X."Circulating microRNAs as biomarkers for the early diagnosis of childhood tuberculosis infection". Molecular Medicine Reports 13.6 (2016): 4620-4626.
Chicago
Zhou, M., Yu, G., Yang, X., Zhu, C., Zhang, Z., Zhan, X."Circulating microRNAs as biomarkers for the early diagnosis of childhood tuberculosis infection". Molecular Medicine Reports 13, no. 6 (2016): 4620-4626. https://doi.org/10.3892/mmr.2016.5097
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