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Hyperoside protects human primary melanocytes against H2O2-induced oxidative damage

  • Authors:
    • Bin Yang
    • Qin Yang
    • Xin Yang
    • Hong‑Bo Yan
    • Qi‑Ping Lu
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Wuhan General Hospital of Guangzhou Command, Wuhan, Hubei 430070, P.R. China, Department of Laboratory Medicine, Wuhan General Hospital of Guangzhou Command, Wuhan, Hubei 430070, P.R. China, Department of General Surgery, Wuhan General Hospital of Guangzhou Command, Wuhan, Hubei 430070, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4613-4619
    |
    Published online on: April 12, 2016
       https://doi.org/10.3892/mmr.2016.5107
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Abstract

Cuscutae semen has been shown to have beneficial effects in the treatment of vitiligo, recorded in the Chinese Pharmacopoeia, whereas the effects of its constituent compounds remains to be elucidated. Using a tetrazolium bromide assay, the present study found that hyperoside (0.5‑200 µg/ml) significantly increased the viability of human melanocytes in a time‑ and dose‑dependent manner. The present study used a cell model of hydrogen peroxide (H2O2)‑induced oxidative damage to examine the effect of hyperoside on human primary melanocytes. The results demonstrated that hyperoside pretreatment for 2 h decreased cell apoptosis from 54.03±9.11 to 17.46±3.10% in the H2O2‑injured melanocytes. The levels of oxidative stress in the mitochondrial membrane potential of the melanocytes increased following hyperoside pretreatment. The mRNA and protein levels of B‑cell lymphoma‑2/Bcl‑2‑associated X protein and caspase 3 were regulated by hyperoside, and phosphoinositide 3‑kinase/AKT and mitogen‑activated protein kinase signaling were also mediated by hyperoside. In conclusion, the results of the present study demonstrated that hyperoside protected the human primary melanocytes against oxidative damage.
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Copy and paste a formatted citation
Spandidos Publications style
Yang B, Yang Q, Yang X, Yan HB and Lu QP: Hyperoside protects human primary melanocytes against H2O2-induced oxidative damage. Mol Med Rep 13: 4613-4619, 2016.
APA
Yang, B., Yang, Q., Yang, X., Yan, H., & Lu, Q. (2016). Hyperoside protects human primary melanocytes against H2O2-induced oxidative damage. Molecular Medicine Reports, 13, 4613-4619. https://doi.org/10.3892/mmr.2016.5107
MLA
Yang, B., Yang, Q., Yang, X., Yan, H., Lu, Q."Hyperoside protects human primary melanocytes against H2O2-induced oxidative damage". Molecular Medicine Reports 13.6 (2016): 4613-4619.
Chicago
Yang, B., Yang, Q., Yang, X., Yan, H., Lu, Q."Hyperoside protects human primary melanocytes against H2O2-induced oxidative damage". Molecular Medicine Reports 13, no. 6 (2016): 4613-4619. https://doi.org/10.3892/mmr.2016.5107
Copy and paste a formatted citation
x
Spandidos Publications style
Yang B, Yang Q, Yang X, Yan HB and Lu QP: Hyperoside protects human primary melanocytes against H2O2-induced oxidative damage. Mol Med Rep 13: 4613-4619, 2016.
APA
Yang, B., Yang, Q., Yang, X., Yan, H., & Lu, Q. (2016). Hyperoside protects human primary melanocytes against H2O2-induced oxidative damage. Molecular Medicine Reports, 13, 4613-4619. https://doi.org/10.3892/mmr.2016.5107
MLA
Yang, B., Yang, Q., Yang, X., Yan, H., Lu, Q."Hyperoside protects human primary melanocytes against H2O2-induced oxidative damage". Molecular Medicine Reports 13.6 (2016): 4613-4619.
Chicago
Yang, B., Yang, Q., Yang, X., Yan, H., Lu, Q."Hyperoside protects human primary melanocytes against H2O2-induced oxidative damage". Molecular Medicine Reports 13, no. 6 (2016): 4613-4619. https://doi.org/10.3892/mmr.2016.5107
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