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Article Open Access

Spot14/Spot14R expression may be involved in MSC adipogenic differentiation in patients with adolescent idiopathic scoliosis

  • Authors:
    • Qifei Wang
    • Junlin Yang
    • Xiang Lin
    • Zifang Huang
    • Chaofan Xie
    • Hengwei Fan
  • View Affiliations / Copyright

    Affiliations: Department of Scoliosis, The First Affiliated Hospital of Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4636-4642
    |
    Published online on: April 12, 2016
       https://doi.org/10.3892/mmr.2016.5109
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Abstract

The aim of the present study was to evaluate the different expression levels of thyroid hormone responsive (THRSP; Spot14)/S14 related, Mig12 (S14R) during bone marrow mesenchymal stem cell (BM-MSC) adipogenesis in adolescent idiopathic scoliosis (AIS) patients. MSCs were retrospectively isolated from AIS patients and controls, and adipogenic differentiation was induced. Total RNA was extracted for Affymetrix 3'‑IVT expression profiling microarrays and compared with the results from healthy controls. The results were confirmed by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) validation and the protein expression levels of Spot14 and its paralogous gene S14R by western blotting and immunohistochemistry. A total of 300 significantly altered mRNAs were detected (111 upregulated and 189 downregulated) and confirmed by RT‑qPCR. The mRNA expression levels of seven genes, including Spot14, were altered by >2‑fold in AIS patients. Spot14/S14R was selected for further investigation. The results of the western blotting demonstrated that mRNA and protein expression levels of Spot14/S14R were significantly higher in AIS patients than the controls (P<0.05). Immunohistochemistry demonstrated Spot14 was expressed in 85% (17/20 cases) in adipose tissue samples from AIS patients and 23.1% (3/13 cases) of adipose tissue samples from controls. The positive ratio of Spot14 in adipose tissue samples from AIS was significantly higher than the controls (P<0.001). The results of the present study indicated that Spot14/S14R were differently expressed in MSC adipogenesis in AIS patients, and they may be important in the abnormal adipogenic differentiation in AIS.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Q, Yang J, Lin X, Huang Z, Xie C and Fan H: Spot14/Spot14R expression may be involved in MSC adipogenic differentiation in patients with adolescent idiopathic scoliosis. Mol Med Rep 13: 4636-4642, 2016.
APA
Wang, Q., Yang, J., Lin, X., Huang, Z., Xie, C., & Fan, H. (2016). Spot14/Spot14R expression may be involved in MSC adipogenic differentiation in patients with adolescent idiopathic scoliosis. Molecular Medicine Reports, 13, 4636-4642. https://doi.org/10.3892/mmr.2016.5109
MLA
Wang, Q., Yang, J., Lin, X., Huang, Z., Xie, C., Fan, H."Spot14/Spot14R expression may be involved in MSC adipogenic differentiation in patients with adolescent idiopathic scoliosis". Molecular Medicine Reports 13.6 (2016): 4636-4642.
Chicago
Wang, Q., Yang, J., Lin, X., Huang, Z., Xie, C., Fan, H."Spot14/Spot14R expression may be involved in MSC adipogenic differentiation in patients with adolescent idiopathic scoliosis". Molecular Medicine Reports 13, no. 6 (2016): 4636-4642. https://doi.org/10.3892/mmr.2016.5109
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Q, Yang J, Lin X, Huang Z, Xie C and Fan H: Spot14/Spot14R expression may be involved in MSC adipogenic differentiation in patients with adolescent idiopathic scoliosis. Mol Med Rep 13: 4636-4642, 2016.
APA
Wang, Q., Yang, J., Lin, X., Huang, Z., Xie, C., & Fan, H. (2016). Spot14/Spot14R expression may be involved in MSC adipogenic differentiation in patients with adolescent idiopathic scoliosis. Molecular Medicine Reports, 13, 4636-4642. https://doi.org/10.3892/mmr.2016.5109
MLA
Wang, Q., Yang, J., Lin, X., Huang, Z., Xie, C., Fan, H."Spot14/Spot14R expression may be involved in MSC adipogenic differentiation in patients with adolescent idiopathic scoliosis". Molecular Medicine Reports 13.6 (2016): 4636-4642.
Chicago
Wang, Q., Yang, J., Lin, X., Huang, Z., Xie, C., Fan, H."Spot14/Spot14R expression may be involved in MSC adipogenic differentiation in patients with adolescent idiopathic scoliosis". Molecular Medicine Reports 13, no. 6 (2016): 4636-4642. https://doi.org/10.3892/mmr.2016.5109
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