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Article

Propofol depresses cisplatin cytotoxicity via the inhibition of gap junctions

  • Authors:
    • Yuan Zhang
    • Xiyan Wang
    • Qin Wang
    • Hui Ge
    • Liang Tao
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, Zhongshan School of Medicine, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China, Tumor Research Institute, Xinjiang Medical University Affiliated Tumor Hospital, Urumqi, Xinjiang 830000, P.R. China
  • Pages: 4715-4720
    |
    Published online on: April 13, 2016
       https://doi.org/10.3892/mmr.2016.5119
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Abstract

The general anesthetic, propofol, affects chemotherapeutic activity, however, the mechanism underlying its effects remains to be fully elucidated. Our previous study showed that tramadol and flurbiprofen depressed the cytotoxicity of cisplatin via the inhibition of gap junction (GJ) intercellular communication (GJIC) in connexin (Cx)32 HeLa cells. The present study investigated whether the effects of propofol on the cytotoxicity of cisplatin were mediated by GJ in U87 glioma cells and Cx26‑transfected HeLa cells. Standard colony formation assay was used to determine the cytotoxicity of cisplatin. Parachute dye coupling assay was used to measure GJ function, and western blot analysis was used to determine the expression levels of Cx32. The results revealed that exposure of the U87 glioma cells and the Cx26-transfected HeLa cells to cisplatin for 1 h reduced clonogenic survival in low density cultures (without GJs) and high density cultures (with GJs). However, the toxic effect was higher in the high density culture. In addition, pretreatment of the cells with propofol significantly reduced cisplatin‑induced cytotoxicity, but only in the presence of functional GJs. Furthermore, propofol significantly inhibited dye coupling through junctional channels, and a long duration of exposure of the cells to propofol downregulated the expression levels of Cx43 and Cx26. These results demonstrated that the inhibition of GJIC by propofol affected the therapeutic efficacy of chemotherapeutic drugs. The present study provides evidence of a novel mechanism underlying the effects of analgesics in counteracting chemotherapeutic efficiency.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Wang X, Wang Q, Ge H and Tao L: Propofol depresses cisplatin cytotoxicity via the inhibition of gap junctions. Mol Med Rep 13: 4715-4720, 2016.
APA
Zhang, Y., Wang, X., Wang, Q., Ge, H., & Tao, L. (2016). Propofol depresses cisplatin cytotoxicity via the inhibition of gap junctions. Molecular Medicine Reports, 13, 4715-4720. https://doi.org/10.3892/mmr.2016.5119
MLA
Zhang, Y., Wang, X., Wang, Q., Ge, H., Tao, L."Propofol depresses cisplatin cytotoxicity via the inhibition of gap junctions". Molecular Medicine Reports 13.6 (2016): 4715-4720.
Chicago
Zhang, Y., Wang, X., Wang, Q., Ge, H., Tao, L."Propofol depresses cisplatin cytotoxicity via the inhibition of gap junctions". Molecular Medicine Reports 13, no. 6 (2016): 4715-4720. https://doi.org/10.3892/mmr.2016.5119
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Wang X, Wang Q, Ge H and Tao L: Propofol depresses cisplatin cytotoxicity via the inhibition of gap junctions. Mol Med Rep 13: 4715-4720, 2016.
APA
Zhang, Y., Wang, X., Wang, Q., Ge, H., & Tao, L. (2016). Propofol depresses cisplatin cytotoxicity via the inhibition of gap junctions. Molecular Medicine Reports, 13, 4715-4720. https://doi.org/10.3892/mmr.2016.5119
MLA
Zhang, Y., Wang, X., Wang, Q., Ge, H., Tao, L."Propofol depresses cisplatin cytotoxicity via the inhibition of gap junctions". Molecular Medicine Reports 13.6 (2016): 4715-4720.
Chicago
Zhang, Y., Wang, X., Wang, Q., Ge, H., Tao, L."Propofol depresses cisplatin cytotoxicity via the inhibition of gap junctions". Molecular Medicine Reports 13, no. 6 (2016): 4715-4720. https://doi.org/10.3892/mmr.2016.5119
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