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Article

The herbal extract KCHO-1 exerts a neuroprotective effect by ameliorating oxidative stress via heme oxygenase-1 upregulation

  • Authors:
    • Dong‑Sung Lee
    • Wonmin Ko
    • Bong‑Keun Song
    • Ilhong Son
    • Dong‑Woung Kim
    • Dae‑Gil Kang
    • Ho‑Sub Lee
    • Hyuncheol Oh
    • Jun‑Hyeog Jang
    • Youn‑Chul Kim
    • Sungchul Kim
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, Chosun University, Dong‑gu, Gwangju 61452, Republic of Korea, Department of Pharmacy, Wonkwang University, Jeollabuk‑do 54538, Republic of Korea, Department of Internal Medicine, School of Oriental Medicine, Wonkwang University, Iksan, Jeollabuk‑do 54538, Republic of Korea, Department of Neurology, Inam Neuroscience Research Center, Sanbon Medical Center, College of Medicine, Wonkwang University, Iksan, Jeollabuk‑do 54538, Republic of Korea, Center of Integrative Medicine, Department of Internal Medicine, Wonkwang University Gwangju Hospital, Gwangju 61729, Republic of Korea, Hanbang Body‑Fluid Research Center, Wonkwang University, Iksan, Jeollabuk‑do 54538, Republic of Korea, Department of Biochemistry, Inha University School of Medicine, Incheon 22212, Republic of Korea, ALS/MND Center of Wonkwang University Korean Medical Hospital, Gwangju 61729, Republic of Korea
  • Pages: 4911-4919
    |
    Published online on: April 14, 2016
       https://doi.org/10.3892/mmr.2016.5129
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Abstract

KCHO-1 is a novel product comprised of 30% ethanol extracts obtained from nine medical herbs, which are commonly used in traditional Korean and Chinese medicine. The nine herbs include Curcuma longa, Salvia miltiorrhiza, Gastrodia elata, Chaenomeles sinensis, Polygala tenuifolia, Paeonia japonica, Glycyrrhiza uralensis, Atractylodes japonica and processed Aconitum carmichaeli. Recent studies have reported the beneficial effects of these herbs. The present study aimed to investigate the direct neuroprotective effects of KCHO‑1 on HT22 mouse hippocampal cells, and to determine the possible underlying mechanisms. KCHO‑1 significantly suppressed glutamate‑ and hydrogen peroxide (H2O2)‑induced cell damage, and reactive oxygen species generation. In addition, KCHO‑1 increased the mRNA and protein expression levels of heme oxygenase (HO)‑1. Tin protoporphyrin, which is an inhibitor of HO activity, partially suppressed the effects of KCHO‑1. Furthermore, KCHO‑1 significantly upregulated nuclear factor erythroid‑derived 2‑related factor‑2 (Nrf2) nuclear translocation. Extracellular signal‑regulated kinase (ERK) activation also appeared to be associated with KCHO‑1‑induced HO‑1 expression, since the ERK inhibitor PD98059 suppressed HO‑1 expression and prevented KCHO‑1‑induced cytoprotection. The results of the present study suggested that KCHO‑1 may effectively prevent glutamate‑ or H2O2‑induced oxidative damage via Nrf2/ERK mitogen‑activated protein kinase‑dependent HO‑1 expression. These data suggest that KCHO‑1 may be useful for the treatment of neurodegenerative diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Lee DS, Ko W, Song BK, Son I, Kim DW, Kang DG, Lee HS, Oh H, Jang JH, Kim YC, Kim YC, et al: The herbal extract KCHO-1 exerts a neuroprotective effect by ameliorating oxidative stress via heme oxygenase-1 upregulation. Mol Med Rep 13: 4911-4919, 2016.
APA
Lee, D., Ko, W., Song, B., Son, I., Kim, D., Kang, D. ... Kim, S. (2016). The herbal extract KCHO-1 exerts a neuroprotective effect by ameliorating oxidative stress via heme oxygenase-1 upregulation. Molecular Medicine Reports, 13, 4911-4919. https://doi.org/10.3892/mmr.2016.5129
MLA
Lee, D., Ko, W., Song, B., Son, I., Kim, D., Kang, D., Lee, H., Oh, H., Jang, J., Kim, Y., Kim, S."The herbal extract KCHO-1 exerts a neuroprotective effect by ameliorating oxidative stress via heme oxygenase-1 upregulation". Molecular Medicine Reports 13.6 (2016): 4911-4919.
Chicago
Lee, D., Ko, W., Song, B., Son, I., Kim, D., Kang, D., Lee, H., Oh, H., Jang, J., Kim, Y., Kim, S."The herbal extract KCHO-1 exerts a neuroprotective effect by ameliorating oxidative stress via heme oxygenase-1 upregulation". Molecular Medicine Reports 13, no. 6 (2016): 4911-4919. https://doi.org/10.3892/mmr.2016.5129
Copy and paste a formatted citation
x
Spandidos Publications style
Lee DS, Ko W, Song BK, Son I, Kim DW, Kang DG, Lee HS, Oh H, Jang JH, Kim YC, Kim YC, et al: The herbal extract KCHO-1 exerts a neuroprotective effect by ameliorating oxidative stress via heme oxygenase-1 upregulation. Mol Med Rep 13: 4911-4919, 2016.
APA
Lee, D., Ko, W., Song, B., Son, I., Kim, D., Kang, D. ... Kim, S. (2016). The herbal extract KCHO-1 exerts a neuroprotective effect by ameliorating oxidative stress via heme oxygenase-1 upregulation. Molecular Medicine Reports, 13, 4911-4919. https://doi.org/10.3892/mmr.2016.5129
MLA
Lee, D., Ko, W., Song, B., Son, I., Kim, D., Kang, D., Lee, H., Oh, H., Jang, J., Kim, Y., Kim, S."The herbal extract KCHO-1 exerts a neuroprotective effect by ameliorating oxidative stress via heme oxygenase-1 upregulation". Molecular Medicine Reports 13.6 (2016): 4911-4919.
Chicago
Lee, D., Ko, W., Song, B., Son, I., Kim, D., Kang, D., Lee, H., Oh, H., Jang, J., Kim, Y., Kim, S."The herbal extract KCHO-1 exerts a neuroprotective effect by ameliorating oxidative stress via heme oxygenase-1 upregulation". Molecular Medicine Reports 13, no. 6 (2016): 4911-4919. https://doi.org/10.3892/mmr.2016.5129
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