The herbal extract KCHO-1 exerts a neuroprotective effect by ameliorating oxidative stress via heme oxygenase-1 upregulation

  • Authors:
    • Dong‑Sung Lee
    • Wonmin Ko
    • Bong‑Keun Song
    • Ilhong Son
    • Dong‑Woung Kim
    • Dae‑Gil Kang
    • Ho‑Sub Lee
    • Hyuncheol Oh
    • Jun‑Hyeog Jang
    • Youn‑Chul Kim
    • Sungchul Kim
  • View Affiliations

  • Published online on: April 14, 2016     https://doi.org/10.3892/mmr.2016.5129
  • Pages: 4911-4919
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

KCHO-1 is a novel product comprised of 30% ethanol extracts obtained from nine medical herbs, which are commonly used in traditional Korean and Chinese medicine. The nine herbs include Curcuma longa, Salvia miltiorrhiza, Gastrodia elata, Chaenomeles sinensis, Polygala tenuifolia, Paeonia japonica, Glycyrrhiza uralensis, Atractylodes japonica and processed Aconitum carmichaeli. Recent studies have reported the beneficial effects of these herbs. The present study aimed to investigate the direct neuroprotective effects of KCHO‑1 on HT22 mouse hippocampal cells, and to determine the possible underlying mechanisms. KCHO‑1 significantly suppressed glutamate‑ and hydrogen peroxide (H2O2)‑induced cell damage, and reactive oxygen species generation. In addition, KCHO‑1 increased the mRNA and protein expression levels of heme oxygenase (HO)‑1. Tin protoporphyrin, which is an inhibitor of HO activity, partially suppressed the effects of KCHO‑1. Furthermore, KCHO‑1 significantly upregulated nuclear factor erythroid‑derived 2‑related factor‑2 (Nrf2) nuclear translocation. Extracellular signal‑regulated kinase (ERK) activation also appeared to be associated with KCHO‑1‑induced HO‑1 expression, since the ERK inhibitor PD98059 suppressed HO‑1 expression and prevented KCHO‑1‑induced cytoprotection. The results of the present study suggested that KCHO‑1 may effectively prevent glutamate‑ or H2O2‑induced oxidative damage via Nrf2/ERK mitogen‑activated protein kinase‑dependent HO‑1 expression. These data suggest that KCHO‑1 may be useful for the treatment of neurodegenerative diseases.
View Figures
View References

Related Articles

Journal Cover

June-2016
Volume 13 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Lee DS, Ko W, Song BK, Son I, Kim DW, Kang DG, Lee HS, Oh H, Jang JH, Kim YC, Kim YC, et al: The herbal extract KCHO-1 exerts a neuroprotective effect by ameliorating oxidative stress via heme oxygenase-1 upregulation. Mol Med Rep 13: 4911-4919, 2016
APA
Lee, D., Ko, W., Song, B., Son, I., Kim, D., Kang, D. ... Kim, S. (2016). The herbal extract KCHO-1 exerts a neuroprotective effect by ameliorating oxidative stress via heme oxygenase-1 upregulation. Molecular Medicine Reports, 13, 4911-4919. https://doi.org/10.3892/mmr.2016.5129
MLA
Lee, D., Ko, W., Song, B., Son, I., Kim, D., Kang, D., Lee, H., Oh, H., Jang, J., Kim, Y., Kim, S."The herbal extract KCHO-1 exerts a neuroprotective effect by ameliorating oxidative stress via heme oxygenase-1 upregulation". Molecular Medicine Reports 13.6 (2016): 4911-4919.
Chicago
Lee, D., Ko, W., Song, B., Son, I., Kim, D., Kang, D., Lee, H., Oh, H., Jang, J., Kim, Y., Kim, S."The herbal extract KCHO-1 exerts a neuroprotective effect by ameliorating oxidative stress via heme oxygenase-1 upregulation". Molecular Medicine Reports 13, no. 6 (2016): 4911-4919. https://doi.org/10.3892/mmr.2016.5129