Open Access

Comparison of molecular mechanisms of rheumatoid arthritis and osteoarthritis using gene microarrays

  • Authors:
    • Hongqiang Li
    • Zhenyong Hao
    • Liqiang Zhao
    • Wei Liu
    • Yanlong Han
    • Yunxing Bai
    • Jian Wang
  • View Affiliations

  • Published online on: April 15, 2016     https://doi.org/10.3892/mmr.2016.5144
  • Pages: 4599-4605
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to compare the molecular mechanisms of rheumatoid arthritis (RA) and osteoarthritis (OA). The microarray dataset no. GSE29746 was downloaded from Gene Expression Omnibus. After data pre‑processing, differential expression analysis between the RA group and the control, as well as between the OA group and the control was performed using the LIMMA package in R and differentially expressed transcripts (DETs) with |log2fold change (FC)|>1 and P<0.01 were identified. DETs screened from each disease group were then subjected to functional annotation using DAVID. Next, DETs from each group were used to construct individual interaction networks using the BIND database, followed by sub‑network mining using clusterONE. Significant functions of nodes in each sub‑network were also investigated. In total, 19 and 281 DETs were screened from the RA and OA groups, respectively, with only six common DETs. DETs from the RA and OA groups were enriched in 8 and 130 gene ontology (GO) terms, respectively, with four common GO terms, of which to were associated with phospholipase C (PLC) activity. In addition, DETs screened from the OA group were enriched in immune response‑associated GO terms, and those screened from the RA group were largely associated with biological processes linked with the cell cycle and chromosomes. Genes involved in PLC activity and its regulation were indicated to be altered in RA as well as in OA. Alterations in the expression of cell cycle‑associated genes were indicated to be linked with the occurrence of OA, while genes participating in the immune response were involved in the occurrence of RA.
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June-2016
Volume 13 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Li H, Hao Z, Zhao L, Liu W, Han Y, Bai Y and Wang J: Comparison of molecular mechanisms of rheumatoid arthritis and osteoarthritis using gene microarrays. Mol Med Rep 13: 4599-4605, 2016
APA
Li, H., Hao, Z., Zhao, L., Liu, W., Han, Y., Bai, Y., & Wang, J. (2016). Comparison of molecular mechanisms of rheumatoid arthritis and osteoarthritis using gene microarrays. Molecular Medicine Reports, 13, 4599-4605. https://doi.org/10.3892/mmr.2016.5144
MLA
Li, H., Hao, Z., Zhao, L., Liu, W., Han, Y., Bai, Y., Wang, J."Comparison of molecular mechanisms of rheumatoid arthritis and osteoarthritis using gene microarrays". Molecular Medicine Reports 13.6 (2016): 4599-4605.
Chicago
Li, H., Hao, Z., Zhao, L., Liu, W., Han, Y., Bai, Y., Wang, J."Comparison of molecular mechanisms of rheumatoid arthritis and osteoarthritis using gene microarrays". Molecular Medicine Reports 13, no. 6 (2016): 4599-4605. https://doi.org/10.3892/mmr.2016.5144