Herbal medicine Gan‑fu‑kang downregulates Wnt/Ca2+ signaling to attenuate liver fibrogenesis in vitro and in vivo

Jia,Yujie; Yuan,Lijun; Xu,Tingting; Li,Hanshu; Yang,Guang; Jiang,Miaona; Zhang,Caihua; Li,Cong

doi:10.3892/mmr.2016.5148

Herbal medicine Gan‑fu‑kang downregulates Wnt/Ca2+ signaling to attenuate liver fibrogenesis in vitro and in vivo

Authors:
- Yujie Jia
- Lijun Yuan
- Tingting Xu
- Hanshu Li
- Guang Yang
- Miaona Jiang
- Caihua Zhang
- Cong Li
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Affiliations: Department of Pathophysiology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China, Department of Hematology, PLA General Hospital, Beijing 100853, P.R. China
Published online on: April 15, 2016 https://doi.org/10.3892/mmr.2016.5148
Pages: 4705-4714

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Abstract

The present study was designed to verify the effect of the Chinese prescription Gan‑fu‑kang (GFK) on the treatment of liver fibrosis, and to investigate its underlying mechanisms. Liver fibrosis was established in rats by the subcutaneous administration of 0.5 mg/kg carbon tetrachloride (CCl4) twice a week for 8 weeks. Subsequently, the rats were divided into four CCl4 groups, which were treated daily with vehicle and GFK (31.25, 312.5 and 3,125 mg/kg/day) orally between weeks 9 and 20. The inhibitory action of GFK‑medicated serum on platelet‑derived growth factor (PDGF)‑stimulated HSC‑T6 cells was also investigated. Biochemical parameters, hydroxyproline (Hyp) content and histological changes to the liver were measured. Reverse transcription‑quantitative polymerase chain reaction, western blotting and immunohistochemistry were used to examine the expression of α‑smooth muscle actin (α‑SMA), PDGF‑BB, PDGF receptor β, collagen type I and II, and the Wnt/Ca2+ signaling pathway. The results showed that GFK significantly alleviated the histological changes, decreased the content of Hyp in the liver and improved liver function in rats. In addition, GFK and GFK‑medicated serum effectively inhibited collagen deposition, reduced the expression of α‑SMA and downregulated the Wnt/Ca2+ signaling pathway in vivo and in vitro, respectively, as well as cell viability (P<0.05). These results indicated that GFK was effective in attenuating liver injury and fibrosis through downregulation of the Wnt/Ca2+ signaling pathway.

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