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Paclitaxel inhibits selenoprotein S expression and attenuates endoplasmic reticulum stress

  • Authors:
    • Hong‑Shuang Qin
    • Pei‑Pei Yu
    • Ying Sun
    • Dan‑Feng Wang
    • Xiao‑Fen Deng
    • Yong‑Li Bao
    • Jun Song
    • Lu‑Guo Sun
    • Zhen‑Bo Song
    • Yu‑Xin Li
  • View Affiliations / Copyright

    Affiliations: National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun, Jilin 130117, P.R. China, Department of Ultrasound, China‑Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China, Research Center of Agriculture and Medicine Gene Engineering of Ministry of Education, Northeast Normal University, Changchun, Jilin 130024, P.R. China
    Copyright: © Qin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5118-5124
    |
    Published online on: April 20, 2016
       https://doi.org/10.3892/mmr.2016.5152
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Abstract

The primary effect of the endoplasmic reticulum (ER) stress response or unfolded protein response (UPR) is to reduce the load of unfolded protein and promote survival. However, prolonged and severe ER stress leads to tissue injury and serious diseases. Thus, it is important to identify drugs that can attenuate ER stress for the treatment of diseases. Natural products continue to provide lead compounds for drug discovery and front‑line pharmacotherapy for people worldwide. Previous studies have indicated that selenoprotein S (SelS) is a sensitive and ideal maker of ER stress. In the present study, a firefly luciferase reporter driven by the SelS gene promoter was used to screen for natural compounds capable of attenuating ER stress. From this, paclitaxel (PTX) was identified to efficiently inhibit the promoter activity of the SelS gene, and further results revealed that PTX significantly inhibited the tunicamycin‑induced upregulation of SelS at the mRNA and protein levels in HepG2 and HEK293T cells. In addition, PTX was able to efficiently inhibit the expression of the ER stress marker, glucose‑regulated protein 78, in ER stress, indicating that PTX may reverse ER stress. Taken together, these results suggest that PTX is able to inhibit SelS expression during ER stress and attenuate ER stress.
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Copy and paste a formatted citation
Spandidos Publications style
Qin HS, Yu PP, Sun Y, Wang DF, Deng XF, Bao YL, Song J, Sun LG, Song ZB, Li YX, Li YX, et al: Paclitaxel inhibits selenoprotein S expression and attenuates endoplasmic reticulum stress. Mol Med Rep 13: 5118-5124, 2016.
APA
Qin, H., Yu, P., Sun, Y., Wang, D., Deng, X., Bao, Y. ... Li, Y. (2016). Paclitaxel inhibits selenoprotein S expression and attenuates endoplasmic reticulum stress. Molecular Medicine Reports, 13, 5118-5124. https://doi.org/10.3892/mmr.2016.5152
MLA
Qin, H., Yu, P., Sun, Y., Wang, D., Deng, X., Bao, Y., Song, J., Sun, L., Song, Z., Li, Y."Paclitaxel inhibits selenoprotein S expression and attenuates endoplasmic reticulum stress". Molecular Medicine Reports 13.6 (2016): 5118-5124.
Chicago
Qin, H., Yu, P., Sun, Y., Wang, D., Deng, X., Bao, Y., Song, J., Sun, L., Song, Z., Li, Y."Paclitaxel inhibits selenoprotein S expression and attenuates endoplasmic reticulum stress". Molecular Medicine Reports 13, no. 6 (2016): 5118-5124. https://doi.org/10.3892/mmr.2016.5152
Copy and paste a formatted citation
x
Spandidos Publications style
Qin HS, Yu PP, Sun Y, Wang DF, Deng XF, Bao YL, Song J, Sun LG, Song ZB, Li YX, Li YX, et al: Paclitaxel inhibits selenoprotein S expression and attenuates endoplasmic reticulum stress. Mol Med Rep 13: 5118-5124, 2016.
APA
Qin, H., Yu, P., Sun, Y., Wang, D., Deng, X., Bao, Y. ... Li, Y. (2016). Paclitaxel inhibits selenoprotein S expression and attenuates endoplasmic reticulum stress. Molecular Medicine Reports, 13, 5118-5124. https://doi.org/10.3892/mmr.2016.5152
MLA
Qin, H., Yu, P., Sun, Y., Wang, D., Deng, X., Bao, Y., Song, J., Sun, L., Song, Z., Li, Y."Paclitaxel inhibits selenoprotein S expression and attenuates endoplasmic reticulum stress". Molecular Medicine Reports 13.6 (2016): 5118-5124.
Chicago
Qin, H., Yu, P., Sun, Y., Wang, D., Deng, X., Bao, Y., Song, J., Sun, L., Song, Z., Li, Y."Paclitaxel inhibits selenoprotein S expression and attenuates endoplasmic reticulum stress". Molecular Medicine Reports 13, no. 6 (2016): 5118-5124. https://doi.org/10.3892/mmr.2016.5152
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