Astragaloside IV prevents high glucose-induced podocyte apoptosis via downregulation of TRPC6

  • Authors:
    • Xing‑Mei Yao
    • Yu‑Jun Liu
    • Yun‑Man Wang
    • Hao Wang
    • Bing‑Bing Zhu
    • Yong‑Ping Liang
    • Wei‑Guo Yao
    • Hui Yu
    • Nian‑Song Wang
    • Xue‑Mei Zhang
    • Wen Peng
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  • Published online on: April 22, 2016     https://doi.org/10.3892/mmr.2016.5167
  • Pages: 5149-5156
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Abstract

Diabetic nephropathy (DN) is one of the most important causes of end‑stage renal disease. Astragaloside IV (AS-IV) is a saponin isolated from Astragalus membranaceus, which possesses various pharmacological activities. AS‑IV prevents podocyte apoptosis and ameliorates renal injury in DN; however, few studies have focused on its effects on ion channels. The transient receptor potential channel 6 (TRPC6) is an important Ca2+‑permeable ion channel in podocytes, which is involved in high glucose (HG)-induced podocyte apoptosis. The aim of the present study was to investigate whether AS‑IV prevented HG‑induced podocyte apoptosis via TRPC6. Cultured podocytes were pre‑treated with 10, 20 or 40 µM AS‑IV for 1 h prior to HG exposure for 24 h. Apoptosis, cell viability, expression of TRPC6, nuclear factor of activated T cells (NFAT2) and B‑cell lymphoma 2‑associated X protein (Bax), as well as the intracellular Ca2+ concentration were subsequently analyzed. The results indicated that HG induced podocyte apoptosis and upregulation of TRPC6, and increased intracellular Ca2+. Furthermore, enhanced NFAT2 and Bax expression was detected. Conversely, AS‑IV protected HG‑induced podocyte apoptosis, downregulated TRPC6 expression and suppressed intracellular Ca2+ in HG-stimulated podocytes. AS‑IV also suppressed NFAT2 and Bax expression. These results suggest that AS‑IV may prevent HG-induced podocyte apoptosis via downregulation of TRPC6, which is possibly mediated via the calcineurin/NFAT signaling pathway.
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June-2016
Volume 13 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Yao XM, Liu YJ, Wang YM, Wang H, Zhu BB, Liang YP, Yao WG, Yu H, Wang NS, Zhang XM, Zhang XM, et al: Astragaloside IV prevents high glucose-induced podocyte apoptosis via downregulation of TRPC6. Mol Med Rep 13: 5149-5156, 2016
APA
Yao, X., Liu, Y., Wang, Y., Wang, H., Zhu, B., Liang, Y. ... Peng, W. (2016). Astragaloside IV prevents high glucose-induced podocyte apoptosis via downregulation of TRPC6. Molecular Medicine Reports, 13, 5149-5156. https://doi.org/10.3892/mmr.2016.5167
MLA
Yao, X., Liu, Y., Wang, Y., Wang, H., Zhu, B., Liang, Y., Yao, W., Yu, H., Wang, N., Zhang, X., Peng, W."Astragaloside IV prevents high glucose-induced podocyte apoptosis via downregulation of TRPC6". Molecular Medicine Reports 13.6 (2016): 5149-5156.
Chicago
Yao, X., Liu, Y., Wang, Y., Wang, H., Zhu, B., Liang, Y., Yao, W., Yu, H., Wang, N., Zhang, X., Peng, W."Astragaloside IV prevents high glucose-induced podocyte apoptosis via downregulation of TRPC6". Molecular Medicine Reports 13, no. 6 (2016): 5149-5156. https://doi.org/10.3892/mmr.2016.5167