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Article

Role of RANKL in the regulation of NFATc1 and c‑Src mRNA expression in osteoclast‑like cells

  • Authors:
    • Jifan Fu
    • You Di Tao
    • Jian Chen
    • Yongsheng Zhang
    • Jianquan He
  • View Affiliations / Copyright

    Affiliations: Department of Rehabilitation, Zhongshan Hospital of Xiamen University, Xiamen University, Xiamen, Fujian 361004, P.R. China
  • Pages: 5163-5168
    |
    Published online on: April 25, 2016
       https://doi.org/10.3892/mmr.2016.5176
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Abstract

This study was designed to determine the effects of receptor activator of nuclear factor κB ligand (RANKL) on the mRNA expression of nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 (NFATc1) and c‑Src in rat osteoclast‑like cells. The marrow cells were exposed to macrophage colony-stimulating factor (M‑CSF; 25 ng/ml) and different concentrations of RANKL (0, 50, 75 and 100 ng/ml) for 9 days. The mRNA expression of NFATc1 and c‑Src was determined by polymerase chain reaction. Compared with the M‑CSF (25 ng/ml)+RANKL (0 ng/ml) group, the levels of NFATc1 and c‑Src mRNA expression were significantly increased in the M‑CSF (25 ng/ml)+RANKL (75 and 100 ng/ml) groups (P<0.01, P<0.01, P<0.01 and P<0.01, respectively). Compared with the M‑CSF (25 ng/ml)+RANKL (50 ng/ml) group, the levels of NFATc1 and c‑Src mRNA expression was significantly increased in the M‑CSF (25 ng/ml)+RANKL (75 and 100 ng/ml) groups (P<0.05, P<0.01, P<0.01 and P<0.01, respectively). Compared with M‑CSF (25 ng/ml)+RANKL (75 ng/ml) group, the levels of NFATc1 and c‑Src mRNA expression was significantly increased in the M‑CSF (25 ng/ml)+RANKL (100 ng/ml) group, (P<0.01 and P<0.01, respectively). These data suggest that RANKL could regulate the expression of NFATc1 and c‑Src mRNA in the marrow culture system.
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Copy and paste a formatted citation
Spandidos Publications style
Fu J, Tao YD, Chen J, Zhang Y and He J: Role of RANKL in the regulation of NFATc1 and c‑Src mRNA expression in osteoclast‑like cells. Mol Med Rep 13: 5163-5168, 2016.
APA
Fu, J., Tao, Y.D., Chen, J., Zhang, Y., & He, J. (2016). Role of RANKL in the regulation of NFATc1 and c‑Src mRNA expression in osteoclast‑like cells. Molecular Medicine Reports, 13, 5163-5168. https://doi.org/10.3892/mmr.2016.5176
MLA
Fu, J., Tao, Y. D., Chen, J., Zhang, Y., He, J."Role of RANKL in the regulation of NFATc1 and c‑Src mRNA expression in osteoclast‑like cells". Molecular Medicine Reports 13.6 (2016): 5163-5168.
Chicago
Fu, J., Tao, Y. D., Chen, J., Zhang, Y., He, J."Role of RANKL in the regulation of NFATc1 and c‑Src mRNA expression in osteoclast‑like cells". Molecular Medicine Reports 13, no. 6 (2016): 5163-5168. https://doi.org/10.3892/mmr.2016.5176
Copy and paste a formatted citation
x
Spandidos Publications style
Fu J, Tao YD, Chen J, Zhang Y and He J: Role of RANKL in the regulation of NFATc1 and c‑Src mRNA expression in osteoclast‑like cells. Mol Med Rep 13: 5163-5168, 2016.
APA
Fu, J., Tao, Y.D., Chen, J., Zhang, Y., & He, J. (2016). Role of RANKL in the regulation of NFATc1 and c‑Src mRNA expression in osteoclast‑like cells. Molecular Medicine Reports, 13, 5163-5168. https://doi.org/10.3892/mmr.2016.5176
MLA
Fu, J., Tao, Y. D., Chen, J., Zhang, Y., He, J."Role of RANKL in the regulation of NFATc1 and c‑Src mRNA expression in osteoclast‑like cells". Molecular Medicine Reports 13.6 (2016): 5163-5168.
Chicago
Fu, J., Tao, Y. D., Chen, J., Zhang, Y., He, J."Role of RANKL in the regulation of NFATc1 and c‑Src mRNA expression in osteoclast‑like cells". Molecular Medicine Reports 13, no. 6 (2016): 5163-5168. https://doi.org/10.3892/mmr.2016.5176
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