Effects of ophiopogonin B on the proliferation and apoptosis of SGC‑7901 human gastric cancer cells

Zhang,Weiyue; Zhang,Qiaoyan; Jiang,Yiping; Li,Feng; Xin,Hailiang

doi:10.3892/mmr.2016.5198

Effects of ophiopogonin B on the proliferation and apoptosis of SGC‑7901 human gastric cancer cells

Authors:
- Weiyue Zhang
- Qiaoyan Zhang
- Yiping Jiang
- Feng Li
- Hailiang Xin
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Affiliations: School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, P.R. China, Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, P.R. China
Published online on: April 27, 2016 https://doi.org/10.3892/mmr.2016.5198
Pages: 4981-4986
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ophiopogonin B (OP‑B) is a bioactive component of Radix Ophiopogon japonicus, which is often used in traditional Chinese medicine to treat cancer. The present study aimed to investigate the antitumor activity of OP‑B in gastric cancer. Cell Counting kit‑8, flow cytometry with Annexin V‑fluorescein isothiocyanate, Hoechst staining, mitochondrial membrane potential (MMP) detection, and reactive oxygen species (ROS) assay were used to detect the biological function of SGC‑7901 gastric cancer cells. The results demonstrated that high concentrations of OP‑B (5, 10 and 20 µmol/l) exerted potent antiproliferative effects on SGC‑7901 cells in a dose‑dependent manner. Furthermore, apoptotic rates were increased and cell morphology was altered following treatment with OP‑B. In addition, OP‑B‑induced apoptosis of SGC‑7901 cells was associated with loss of MMP and increased ROS generation. Western blotting indicated that treatment with OP‑B increased the protein expression levels of caspase‑3 and B‑cell lymphoma 2 (Bcl‑2)‑associated X protein, whereas the expression levels of Bcl‑2 and the phosphorylation levels of extracellular signal‑regulated kinases 1/2 and c‑Jun N‑terminal kinases 1/2 were decreased. These results suggest that OP‑B may be considered a potential inhibitor of gastric cancer progression, and may be used as an alternative compound for its treatment.

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1	Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI
2	de Martel C, Forman D and Plummer M: Gastric cancer: Epidemiology and risk factors. Gastroenterol Clin North Am. 42:219–240. 2013. View Article : Google Scholar : PubMed/NCBI
3	Wong H and Yau T: Targeted therapy in the management of advanced gastric cancer: Are we making progress in the era of personalized medicine? Oncologist. 17:346–358. 2012. View Article : Google Scholar : PubMed/NCBI
4	Qi F, Li A, Inagaki Y, Gao J, Li J, Kokudo N, Li XK and Tang W: Chinese herbal medicines as adjuvant treatment during chemo-or radio-therapy for cancer. Biosci Trends. 4:297–307. 2010.
5	Li R, Chen WC, Wang WP, Tian WY and Zhang XG: Extraction, characterization of Astragalus polysaccharides and its immune modulating activities in rats with gastric cancer. Carbohydr Polym. 78:738–742. 2009. View Article : Google Scholar
6	Li X, Yang G, Li X, Zhang Y, Yang J, Chang J, Sun X, Zhou X, Guo Y, Xu Y, et al: Traditional Chinese medicine in cancer care: A review of controlled clinical studies published in Chinese. PLoS One. 8:e603382013. View Article : Google Scholar : PubMed/NCBI
7	Ling Y: Traditional Chinese medicine in the treatment of symptoms in patients with advanced cancer. Ann Palliat Med. 2:141–152. 2013.PubMed/NCBI
8	Boyang Y and Guojun X: Studies on resource utilization of Chinese drug dwarf lilyturf (Ophiopogon japonicus). Zhongyao Maidong De Ziyuan Liyong Yanjiu. 26:205–210. 1995.In Chinese.
9	Xiao P, Li DP and Yang SL: Modern Chinese materia medica. Chemical Industry Press. 4:253–272. 2002.
10	Kou J, Sun Y, Lin Y, Cheng Z, Zheng W, Yu B and Xu Q: Anti-inflammatory activities of aqueous extract from Radix Ophiopogon japonicus and its two constituents. Biol Pharm Bull. 28:1234–1238. 2005. View Article : Google Scholar : PubMed/NCBI
11	Lin X, Zhou QF and Xu D: Research progress of the pharmacological actions of ophiopogon root. Shang Hai Zhong Yi Yao Za Zhi She. 38:59–61. 2004.In Chinese.
12	Chen M, Du Y, Qui M, Wang M, Chen K, Huang Z, Jiang M, Xiong F, Chen J, Zhou J, et al: Ophiopogonin B-induced autophagy in non-small cell lung cancer cells via inhibition of the PI3 K/Akt signaling pathway. Oncol Rep. 29:430–436. 2013.
13	Xu QJ, Hou LL, Hu GQ and Xie SQ: Molecular mechanism of ophiopogonin B induced cellular autophagy of human cervical cancer HeLa cells. Yao Xue Xue Bao. 48:855–859. 2013.In Chinese. PubMed/NCBI
14	Kabeer FA, Sreedevi GB, Nair MS, Rajalekshmi DS, Gopalakrishnan LP, Kunjuraman S and Prathapan R: Antineoplastic effects of deoxyelephantopin, a sesquiterpene lactone from Elephantopus scaber, on lung adenocarcinoma (A549) cells. J Integr Med. 11:269–277. 2013. View Article : Google Scholar : PubMed/NCBI
15	Mondal J, Bishayee K, Panigrahi AK and Khuda-Bukhsh AR: Low doses of ethanolic extract of Boldo (Peumus boldus) can ameliorate toxicity generated by cisplatin in normal liver cells of mice in vivo and in WRL-68 cells in vitro, but not in cancer cells in vivo or in vitro. J Integr Med. 12:425–438. 2014. View Article : Google Scholar : PubMed/NCBI
16	Wang YH, Qiu C, Wang DW, Hu ZF, Yu BY and Zhu DN: Identification of multiple constituents in the traditional Chinese medicine formula Sheng-Mai San and rat plasma after oral administration by HPLC-DAD-MS/MS. J Pharm Biomed Anal. 54:1110–1127. 2011. View Article : Google Scholar
17	Thompson CB: Apoptosis in the pathogenesis and treatment of disease. Science. 267:1456–1462. 1995. View Article : Google Scholar : PubMed/NCBI
18	Mohamad N, Gutiérrez A, Núñez M, Cocca C, Martín G, Cricco G, Medina V, Rivera E and Bergoc R: Mitochondrial apoptotic pathways. Biocell. 29:149–161. 2005.PubMed/NCBI
19	Lucken-Ardjomande S and Martinou JC: Newcomers in the process of mitochondrial permeabilization. J Cell Sci. 118:473–483. 2005. View Article : Google Scholar : PubMed/NCBI
20	Ott M, Gogvadze V, Orrenius S and Zhivotovsky B: Mitochondria, oxidative stress and cell death. Apoptosis. 12:913–922. 2007. View Article : Google Scholar : PubMed/NCBI
21	Götz ME, Künig G, Riederer P and Youdim MB: Oxidative stress: Free radical production in neural degeneration. Pharmacol Ther. 63:37–122. 1994. View Article : Google Scholar : PubMed/NCBI
22	Cagnol S and Chambard JC: ERK and cell death: Mechanisms of ERK-induced cell death-apoptosis, autophagy and senescence. FEBS J. 277:2–21. 2010. View Article : Google Scholar
23	Kralova J, Dvorak M, Koc M and Kral V: P38 MAPK plays an essential role in apoptosis induced by photoactivation of a novel ethylene glycol porphyrin derivative. Oncogene. 27:3010–3020. 2008. View Article : Google Scholar
24	Dhanasekaran DN and Reddy EP: JNK signaling in apoptosis. Oncogene. 27:6245–6251. 2008. View Article : Google Scholar : PubMed/NCBI