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Trichostatin A potentiates genistein-induced apoptosis and reverses EMT in HEp2 cells

  • Authors:
    • Ruixia Du
    • Zhe Liu
    • Xuedong Hou
    • Gongbi Fu
    • Ning An
    • Liping Wang
  • View Affiliations / Copyright

    Affiliations: Department of Otorhinolaryngology, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, P.R. China, Department of Pancreatic Surgery, First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Otorhinolaryngology, Fengtian Hospital, Shenyang Medical University, Shenyang, Liaoning 110024, P.R. China
    Copyright: © Du et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5045-5052
    |
    Published online on: April 28, 2016
       https://doi.org/10.3892/mmr.2016.5204
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Abstract

Genistein and trichostatin A (TSA) are two chemotherapeutic compounds with antitumor effects in different types of cancer cell. However, the effects of genistein and TSA on the HEp‑2 laryngeal cancer cell line remain to be fully elucidated. In the present study, it was found that genistein and TSA inhibited cell growth and cell migration, and promoted apoptosis in the HEp‑2 laryngeal cancer cell line. The HEp‑2 cells were treated with genistein, TSA or the two compounds in combination. Cell proliferation and apoptosis were measured using an MTT assay, Annexin V/propidium iodide staining and a TUNEL assay. Cell invasion was determined using a Matrigel‑based Transwell assay. Western blotting was used to examine the activation of the Akt pathway and the expression levels of pro‑or anti‑apoptotic proteins. Treatment with either genistein or TSA alone mildly inhibited cell viability, growth and invasion, and induced the apoptosis of the laryngeal cancer cells, whereas more marked effects were observed in the cells treated with the combination of the two compounds. In addition, genistein reversed endothelial growth factor‑induced epithelial‑mesenchymal transition (EMT) in the HEp‑2 cells, the effect of which were was further increased by joint application with TSA. Treatment of the HEp‑2 cells with genistein and TSA led to a significant reduction in the phosphorylation of Akt and activation of its downstream target, and resulted in peroxisome proliferator‑activated receptor‑γ cleavage, increased expression of B cell lymphoma‑2 (Bcl‑2)‑associated X protein and reduced the expression of Bcl‑2. In conclusion, the present study demonstrated that, with the involvement of TSA, genistein exhibited substantial advantages in inhibiting laryngeal carcinoma cell growth, invasion and EMT, and induced apoptosis, compared with genistein treatment alone, which occurred through the regulation of Akt activation and the apoptotic pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Du R, Liu Z, Hou X, Fu G, An N and Wang L: Trichostatin A potentiates genistein-induced apoptosis and reverses EMT in HEp2 cells. Mol Med Rep 13: 5045-5052, 2016.
APA
Du, R., Liu, Z., Hou, X., Fu, G., An, N., & Wang, L. (2016). Trichostatin A potentiates genistein-induced apoptosis and reverses EMT in HEp2 cells. Molecular Medicine Reports, 13, 5045-5052. https://doi.org/10.3892/mmr.2016.5204
MLA
Du, R., Liu, Z., Hou, X., Fu, G., An, N., Wang, L."Trichostatin A potentiates genistein-induced apoptosis and reverses EMT in HEp2 cells". Molecular Medicine Reports 13.6 (2016): 5045-5052.
Chicago
Du, R., Liu, Z., Hou, X., Fu, G., An, N., Wang, L."Trichostatin A potentiates genistein-induced apoptosis and reverses EMT in HEp2 cells". Molecular Medicine Reports 13, no. 6 (2016): 5045-5052. https://doi.org/10.3892/mmr.2016.5204
Copy and paste a formatted citation
x
Spandidos Publications style
Du R, Liu Z, Hou X, Fu G, An N and Wang L: Trichostatin A potentiates genistein-induced apoptosis and reverses EMT in HEp2 cells. Mol Med Rep 13: 5045-5052, 2016.
APA
Du, R., Liu, Z., Hou, X., Fu, G., An, N., & Wang, L. (2016). Trichostatin A potentiates genistein-induced apoptosis and reverses EMT in HEp2 cells. Molecular Medicine Reports, 13, 5045-5052. https://doi.org/10.3892/mmr.2016.5204
MLA
Du, R., Liu, Z., Hou, X., Fu, G., An, N., Wang, L."Trichostatin A potentiates genistein-induced apoptosis and reverses EMT in HEp2 cells". Molecular Medicine Reports 13.6 (2016): 5045-5052.
Chicago
Du, R., Liu, Z., Hou, X., Fu, G., An, N., Wang, L."Trichostatin A potentiates genistein-induced apoptosis and reverses EMT in HEp2 cells". Molecular Medicine Reports 13, no. 6 (2016): 5045-5052. https://doi.org/10.3892/mmr.2016.5204
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