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Article Open Access

Single nucleotide polymorphism-based microarray analysis for the diagnosis of hydatidiform moles

  • Authors:
    • Yingjun Xie
    • Xiaojuan Pei
    • Yu Dong
    • Huiqun Wu
    • Jianzhu Wu
    • Huijuan Shi
    • Xuying Zhuang
    • Xiaofang Sun
    • Jialing He
  • View Affiliations / Copyright

    Affiliations: Fetal Medicine Center, The First Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510150, P.R. China, Department of Pathology, The Huizhou Municipal Center People Hospital, Huizhou, Guangdong 516001, P.R. China, Department of Pathology, The First Affiliated Hospital of Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China, Tianjin Public Security Traffic Management Bureau, Tianjin 300241, P.R. China, Key Laboratory for Major Obstetric Diseases of Guangdong Province, Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510150, P.R. China, Experimental Animal Center, Research Institute for National Health and Family Planning Commission, Beijing 100081, P.R. China
    Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 137-144
    |
    Published online on: May 5, 2016
       https://doi.org/10.3892/mmr.2016.5211
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Abstract

In clinical diagnostics, single nucleotide polymorphism (SNP)-based microarray analysis enables the detection of copy number variations (CNVs), as well as copy number neutral regions, that are absent of heterozygosity throughout the genome. The aim of the present study was to evaluate the effectiveness and sensitivity of SNP‑based microarray analysis in the diagnosis of hydatidiform mole (HM). By using whole‑genome SNP microarray analysis, villous genotypes were detected, and the ploidy of villous tissue was determined to identify HMs. A total of 66 villous tissues and two twin tissues were assessed in the present study. Among these samples, 11 were triploid, one was tetraploid, 23 were abnormal aneuploidy, three were complete genome homozygosity, and the remaining ones were normal ploidy. The most noteworthy finding of the present study was the identification of six partial HMs and three complete HMs from those samples that were not identified as being HMs on the basis of the initial diagnosis of experienced obstetricians. This study has demonstrated that the application of an SNP‑based microarray analysis was able to increase the sensitivity of diagnosis for HMs with partial and complete HMs, which makes the identification of these diseases at an early gestational age possible.
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Copy and paste a formatted citation
Spandidos Publications style
Xie Y, Pei X, Dong Y, Wu H, Wu J, Shi H, Zhuang X, Sun X and He J: Single nucleotide polymorphism-based microarray analysis for the diagnosis of hydatidiform moles. Mol Med Rep 14: 137-144, 2016.
APA
Xie, Y., Pei, X., Dong, Y., Wu, H., Wu, J., Shi, H. ... He, J. (2016). Single nucleotide polymorphism-based microarray analysis for the diagnosis of hydatidiform moles. Molecular Medicine Reports, 14, 137-144. https://doi.org/10.3892/mmr.2016.5211
MLA
Xie, Y., Pei, X., Dong, Y., Wu, H., Wu, J., Shi, H., Zhuang, X., Sun, X., He, J."Single nucleotide polymorphism-based microarray analysis for the diagnosis of hydatidiform moles". Molecular Medicine Reports 14.1 (2016): 137-144.
Chicago
Xie, Y., Pei, X., Dong, Y., Wu, H., Wu, J., Shi, H., Zhuang, X., Sun, X., He, J."Single nucleotide polymorphism-based microarray analysis for the diagnosis of hydatidiform moles". Molecular Medicine Reports 14, no. 1 (2016): 137-144. https://doi.org/10.3892/mmr.2016.5211
Copy and paste a formatted citation
x
Spandidos Publications style
Xie Y, Pei X, Dong Y, Wu H, Wu J, Shi H, Zhuang X, Sun X and He J: Single nucleotide polymorphism-based microarray analysis for the diagnosis of hydatidiform moles. Mol Med Rep 14: 137-144, 2016.
APA
Xie, Y., Pei, X., Dong, Y., Wu, H., Wu, J., Shi, H. ... He, J. (2016). Single nucleotide polymorphism-based microarray analysis for the diagnosis of hydatidiform moles. Molecular Medicine Reports, 14, 137-144. https://doi.org/10.3892/mmr.2016.5211
MLA
Xie, Y., Pei, X., Dong, Y., Wu, H., Wu, J., Shi, H., Zhuang, X., Sun, X., He, J."Single nucleotide polymorphism-based microarray analysis for the diagnosis of hydatidiform moles". Molecular Medicine Reports 14.1 (2016): 137-144.
Chicago
Xie, Y., Pei, X., Dong, Y., Wu, H., Wu, J., Shi, H., Zhuang, X., Sun, X., He, J."Single nucleotide polymorphism-based microarray analysis for the diagnosis of hydatidiform moles". Molecular Medicine Reports 14, no. 1 (2016): 137-144. https://doi.org/10.3892/mmr.2016.5211
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