Ratio of S-adenosylmethionine to S-adenosylhomocysteine as a sensitive indicator of atherosclerosis

  • Authors:
    • Huiping Zhang
    • Zhihong Liu
    • Shengchao Ma
    • Hui Zhang
    • Fanqi Kong
    • Yangyang He
    • Xiaoling Yang
    • Yanhua Wang
    • Hua Xu
    • Anning Yang
    • Jue Tian
    • Minghao Zhang
    • Jun Cao
    • Yideng Jiang
    • Xiong Guo
  • View Affiliations

  • Published online on: May 9, 2016     https://doi.org/10.3892/mmr.2016.5230
  • Pages: 289-300
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Abstract

The present study aimed to confirm whether the ratio of S-adenosylmethionine (SAM) to S-adenosylhomocysteine (SAH) is a sensitive indicator, and whether it can be used as a biomarker for the clinical diagnosis of atherosclerosis. Apolipoprotein E (ApoE)-/- mice were randomly divided into four groups and fed with a high methionine diet for 15 weeks. Serum levels of homocysteine (Hcy) were measured using an automatic biochemistry analyzer. The concentrations of SAM and SAH were determined using high‑performance liquid chromatography. The methylation levels of B1 repetitive elements, adipocyte fatty acid binding protein (FABP4), monocyte chemoattractant protein-1 (MCP-1) and extracellular superoxide dismutase (EC‑SOD) were analyzed using nested touchdown-methylation-specific-polymerase chain reaction analysis. After 15 weeks, compared with the normal control group, serum concentrations of Hcy were significantly increased by 1.15‑, 2.54‑ and 1.17‑fold (P<0.05) in the ApoE‑/‑ control group, Meth group and Meth‑F group, respectively. The sizes of the atherosclerotic lesions were increased in the ApoE‑/‑ control group, Meth group and Meth‑F group, by up to 1.44‑, 2.40‑ and 1.45‑fold, respectively, compared with the normal control group (P<0.05). The concentrations of SAM were significantly increased by 3.02‑, 3.42‑ and 2.46‑fold in the ApoE‑/‑ control group, Meth group and Meth‑F group, respectively (P<0.05). The ratios of SAM/SAH were increased by 1.67‑ and 2.75‑fold in the in ApoE‑/‑ control group and Meth group, respectively, compared with the normal control group. The methylation levels of B1 repetitive elements, FABP4, MCP‑1 and EC‑SOD were decreased and exhibited hypomethylation. The methylation statuses of these genes were correlated with the ratio of the serum levels of SAM and SAH. These findings suggested that the SAM/SAH ratio is a biomarker and may provide a sensitive indicator for the clinical diagnosis of atherosclerosis.
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July-2016
Volume 14 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhang H, Liu Z, Ma S, Zhang H, Kong F, He Y, Yang X, Wang Y, Xu H, Yang A, Yang A, et al: Ratio of S-adenosylmethionine to S-adenosylhomocysteine as a sensitive indicator of atherosclerosis. Mol Med Rep 14: 289-300, 2016
APA
Zhang, H., Liu, Z., Ma, S., Zhang, H., Kong, F., He, Y. ... Guo, X. (2016). Ratio of S-adenosylmethionine to S-adenosylhomocysteine as a sensitive indicator of atherosclerosis. Molecular Medicine Reports, 14, 289-300. https://doi.org/10.3892/mmr.2016.5230
MLA
Zhang, H., Liu, Z., Ma, S., Zhang, H., Kong, F., He, Y., Yang, X., Wang, Y., Xu, H., Yang, A., Tian, J., Zhang, M., Cao, J., Jiang, Y., Guo, X."Ratio of S-adenosylmethionine to S-adenosylhomocysteine as a sensitive indicator of atherosclerosis". Molecular Medicine Reports 14.1 (2016): 289-300.
Chicago
Zhang, H., Liu, Z., Ma, S., Zhang, H., Kong, F., He, Y., Yang, X., Wang, Y., Xu, H., Yang, A., Tian, J., Zhang, M., Cao, J., Jiang, Y., Guo, X."Ratio of S-adenosylmethionine to S-adenosylhomocysteine as a sensitive indicator of atherosclerosis". Molecular Medicine Reports 14, no. 1 (2016): 289-300. https://doi.org/10.3892/mmr.2016.5230