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July-2016 Volume 14 Issue 1

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Article

Lentivirus-mediated inhibition of USP39 suppresses the growth of gastric cancer cells via PARP activation

  • Authors:
    • Xinbao Wang
    • Qiming Yu
    • Ling Huang
    • Pengfei Yu
  • View Affiliations / Copyright

    Affiliations: Department of Abdominal Tumor Surgery, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China
  • Pages: 301-306
    |
    Published online on: May 12, 2016
       https://doi.org/10.3892/mmr.2016.5252
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Abstract

Gastric cancer (GC) is the second most common cause of cancer-associated mortality worldwide. Ubiquitin-specific peptidase 39 (USP39) has important roles in mRNA processing and has been reported to be involved in the growth of breast cancer cells. However, the roles of USP39 in GC have remained to be investigated, which was the aim of the present study. A lentivirus expressing short hairpin RNA targeting USP39 was constructed and transfected into MGC80‑3 cells. Suppression of USP39 expression significantly decreased the proliferation and colony forming ability of MGC80‑3 cells as indicated by an MTT and a clonogenic assay, respectively. In addition, flow cytometric cell cycle analysis revealed that depression of USP39 induced G2/M‑phase arrest, while an intracellular signaling array showed that the cleavage of PARP at Asp214 was increased following USP39 knockdown. These results suggested that USP39 is involved in the proliferation of GCs and may be utilized as a molecular target for GC therapy.
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1 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Nishida T, Tsutsui S, Kato M, Inoue T, Yamamoto S, Hayashi Y, Akasaka T, Yamada T, Shinzaki S, Iijima H, et al: Treatment strategy for gastric non-invasive intraepithelial neoplasia diagnosed by endoscopic biopsy. J Gastrointest Pathophysiol. 2:93–99. 2011. View Article : Google Scholar

3 

Layke JC and Lopez PP: Gastric cancer: Diagnosis and treatment options. Am Fam Physician. 69:1133–1140. 2004.PubMed/NCBI

4 

Cheng L, Wang P, Yang S, Yang Y and Zhang Q, Zhang W, Xiao H, Gao H and Zhang Q: Identification of genes with a correlation between copy number and expression in gastric cancer. BMC Med Genomics. 5:142012. View Article : Google Scholar : PubMed/NCBI

5 

Brenner H, Rothenbacher D and Arndt V: Epidemiology of stomach cancer. Methods Mol Biol. 472:467–477. 2009. View Article : Google Scholar

6 

Yeh ET: SUMOylation and De-SUMOylation: Wrestling with life's processes. J Biol Chem. 284:8223–8227. 2009. View Article : Google Scholar :

7 

Nie M, Xie Y, Loo JA and Courey AJ: Genetic and proteomic evidence for roles of Drosophila SUMO in cell cycle control, Ras signaling and early pattern formation. PLoS One. 4:e59052009. View Article : Google Scholar

8 

Johnson ES: Protein modification by SUMO. Annu Rev Biochem. 73:355–382. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Yang W and Paschen W: SUMO proteomics to decipher the SUMO-modified proteome regulated by various diseases. Proteomics. 15:1181–1191. 2015. View Article : Google Scholar :

10 

Nijman SM, Luna-Vargas MP, Velds A, Brummelkamp TR, Dirac AM, Sixma TK and Bernards R: A genomic and functional inventory of deubiquitinating enzymes. Cell. 123:773–786. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Wilkinson KD: Regulation of ubiquitin-dependent processes by deubiquitinating enzymes. FASEB J. 11:1245–1256. 1997.PubMed/NCBI

12 

Makarova OV, Makarov EM and Lührmann R: The 65 and 110 kDa SR-related proteins of the U4/U6.U5 tri-snRNP are essential for the assembly of mature spliceosomes. EMBO J. 20:2553–2563. 2001. View Article : Google Scholar : PubMed/NCBI

13 

van Leuken RJ, Luna-Vargas MP, Sixma TK, Wolthuis RM and Medema RH: Usp39 is essential for mitotic spindle checkpoint integrity and controls mRNA-levels of aurora B. Cell Cycle. 7:2710–2719. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Rios Y, Melmed S, Lin S and Liu NA: Zebrafish usp39 mutation leads to rb1 mRNA splicing defect and pituitary lineage expansion. PLoS Genet. 7:e10012712011. View Article : Google Scholar : PubMed/NCBI

15 

Wang H, Ji X, Liu X, Yao R, Chi J, Liu S, Wang Y, Cao W and Zhou Q: Lentivirus-mediated inhibition of USP39 suppresses the growth of breast cancer cells in vitro. Oncol Rep. 30:2871–2847. 2013.PubMed/NCBI

16 

Wen D, Xu Z, Xia L, Liu X, Tu Y, Lei H, Wang W, Wang T, Song L, Ma C, et al: Important role of SUMOylation of Spliceosome factors in prostate cancer cells. J Proteome Res. 13:3571–3582. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Wang X, Yu Q, Zhang Y, Ling Z and Yu P: Tectonic 1 accelerates gastric cancer cell proliferation and cell cycle progression in vitro. Mol Med Rep. 12:5897–5902. 2015.PubMed/NCBI

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar

19 

Sallmann FR, Bourassa S, Saint-Cyr J and Poirier GG: Characterization of antibodies specific for the caspase cleavage site on poly(ADP-ribose) polymerase: specific detection of apoptotic fragments and mapping of the necrotic fragments of poly(ADP-ribose) polymerase. Biochem Cell Biol. 75:451–456. 1997. View Article : Google Scholar : PubMed/NCBI

20 

Zhang Q, Li Y, Li X, Zhou W, Shi B, Chen H and Yuan W: PARP-1 Val762Ala polymorphism, CagA+ H. pylori infection and risk for gastric cancer in Han Chinese population. Mol Biol Rep. 36:1461–1467. 2009. View Article : Google Scholar

21 

Kim J, Pyun JA, Cho SW, Lee K and Kwack K: Lymph node metastasis of gastric cancer is associated with the interaction between poly (ADP-ribose) polymerase 1 and matrix metallopeptidase 2. DNA Cell Biol. 30:1011–1017. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Tao Z, Gao P and Liu HW: Studies of the expression of human poly(ADP-ribose) polymerase-1 in Saccharomyces cerevisiae and identification of PARP-1 substrates by yeast proteome microarray screening. Biochemistry. 48:11745–11754. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Nozaki T, Masutani M, Akagawa T, Sugimura T and Esumi H: Suppression of G1 arrest and enhancement of G2 arrest by inhibitors of poly(ADP-ribose) polymerase: Possible involvement of poly(ADP-ribosyl)ation in cell cycle arrest following gamma-irradiation. Jpn J Cancer Res. 85:1094–1098. 1994. View Article : Google Scholar : PubMed/NCBI

24 

Piao L, Nakagawa H, Ueda K, Chung S, Kashiwaya K, Eguchi H, Ohigashi H, Ishikawa O, Daigo Y, Matsuda K and Nakamura Y: C12orf48, termed PARP-1 binding protein, enhances poly(ADP-ribose) polymerase-1 (PARP-1) activity and protects pancreatic cancer cells from DNA damage. Genes Chromosomes Cancer. 50:13–24. 2011. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Yu Q, Huang L and Yu P: Lentivirus-mediated inhibition of USP39 suppresses the growth of gastric cancer cells via PARP activation. Mol Med Rep 14: 301-306, 2016.
APA
Wang, X., Yu, Q., Huang, L., & Yu, P. (2016). Lentivirus-mediated inhibition of USP39 suppresses the growth of gastric cancer cells via PARP activation. Molecular Medicine Reports, 14, 301-306. https://doi.org/10.3892/mmr.2016.5252
MLA
Wang, X., Yu, Q., Huang, L., Yu, P."Lentivirus-mediated inhibition of USP39 suppresses the growth of gastric cancer cells via PARP activation". Molecular Medicine Reports 14.1 (2016): 301-306.
Chicago
Wang, X., Yu, Q., Huang, L., Yu, P."Lentivirus-mediated inhibition of USP39 suppresses the growth of gastric cancer cells via PARP activation". Molecular Medicine Reports 14, no. 1 (2016): 301-306. https://doi.org/10.3892/mmr.2016.5252
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Yu Q, Huang L and Yu P: Lentivirus-mediated inhibition of USP39 suppresses the growth of gastric cancer cells via PARP activation. Mol Med Rep 14: 301-306, 2016.
APA
Wang, X., Yu, Q., Huang, L., & Yu, P. (2016). Lentivirus-mediated inhibition of USP39 suppresses the growth of gastric cancer cells via PARP activation. Molecular Medicine Reports, 14, 301-306. https://doi.org/10.3892/mmr.2016.5252
MLA
Wang, X., Yu, Q., Huang, L., Yu, P."Lentivirus-mediated inhibition of USP39 suppresses the growth of gastric cancer cells via PARP activation". Molecular Medicine Reports 14.1 (2016): 301-306.
Chicago
Wang, X., Yu, Q., Huang, L., Yu, P."Lentivirus-mediated inhibition of USP39 suppresses the growth of gastric cancer cells via PARP activation". Molecular Medicine Reports 14, no. 1 (2016): 301-306. https://doi.org/10.3892/mmr.2016.5252
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