Expression and function of microRNA-497 in human osteosarcoma

  • Authors:
    • Qi Liu
    • Huan Wang
    • Ankit Singh
    • Fenyong Shou
  • View Affiliations

  • Published online on: May 12, 2016     https://doi.org/10.3892/mmr.2016.5256
  • Pages: 439-445
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Abstract

The expression and function of microRNA-497 (miR-497) has previously been reported in various types of human cancer; however, miR-497 has not previously been investigated in human osteosarcoma (OS). In the present study, the expression levels of miR‑497 were analyzed by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) in OS tissues and cell lines. In addition, post-transfection with miR‑497, RT‑qPCR, cell proliferation, migration and invasion assays, western blot analysis, and luciferase assays were performed in OS cell lines. The results of the present study demonstrated that miR‑497 was downregulated in OS tissues and cells compared with normal controls. Furthermore, upregulation of miR‑497 inhibited cell proliferation, migration and invasion in osteosarcoma cell lines compared with the negative control group. In addition, the present study demonstrated that miR‑497 may function by directly targeting insulin‑like growth factor 1 receptor in OS cells. These findings indicated that miR‑497 may be useful as a therapeutic target for the treatment of OS.
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July-2016
Volume 14 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Liu Q, Wang H, Singh A and Shou F: Expression and function of microRNA-497 in human osteosarcoma. Mol Med Rep 14: 439-445, 2016
APA
Liu, Q., Wang, H., Singh, A., & Shou, F. (2016). Expression and function of microRNA-497 in human osteosarcoma. Molecular Medicine Reports, 14, 439-445. https://doi.org/10.3892/mmr.2016.5256
MLA
Liu, Q., Wang, H., Singh, A., Shou, F."Expression and function of microRNA-497 in human osteosarcoma". Molecular Medicine Reports 14.1 (2016): 439-445.
Chicago
Liu, Q., Wang, H., Singh, A., Shou, F."Expression and function of microRNA-497 in human osteosarcoma". Molecular Medicine Reports 14, no. 1 (2016): 439-445. https://doi.org/10.3892/mmr.2016.5256