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The prognostic role and reduced expression of FOXJ2 in human hepatocellular carcinoma

  • Authors:
    • Zhongbao Zhang
    • Guangju Meng
    • Liang Wang
    • Yingying Ma
    • Zhongzheng Guan
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The Third People's Hospital of Liaocheng, Liaocheng, Shandong 252000, P.R. China, Department of Infectious Diseases, Tai'an Center Hospital, Tai'an, Shandong 271000, P.R. China, Department of Surgery, The Third People's Hospital of Liaocheng, Liaocheng, Shandong 252000, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 254-262
    |
    Published online on: May 12, 2016
       https://doi.org/10.3892/mmr.2016.5261
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Abstract

The current study aimed to investigate the potential role of the FOXJ2 (forkhead box J2) protein in the pathology of hepatocellular carcinoma (HCC). Western blotting was performed to determine the expression levels of FOXJ2 in HCC tissues and HCC cells. Specimens from 110 patients with HCC undergoing hepatic resection were evaluated for FOXJ2 expression using an immunohistochemical assay. The correlation between FOXJ2 expression and clinicopathological factors of the patients was determined by statistical analysis to determine the prognostic merit of FOXJ2 expression in HCC. The detailed involvement of FOXJ2 in the regulation of HCC proliferation was further investigated using FOXJ2‑targeting small interfering RNA (siRNA). FOXJ2 protein was identified to be significantly downregulated in HCC tissues compared with adjacent normal liver tissues. Immunohistochemical analysis demonstrated that the expression of FOXJ2 was negatively correlated with Ki‑67 levels in HCC specimens (r=‑0.679, P<0.001). Furthermore, statistical analysis indicated FOXJ2 expression was significantly associated with histological differentiation (P=0.005), the size of largest tumor (P=0.002) and metastasis (P=0.036). Using Kaplan‑Meier analysis, it was demonstrated that high FOXJ2 expression levels predicted significantly improved patient survival rates compared with low FOXJ2 expression levels (P<0.001). In addition, it was observed that interference of FOXJ2 expression using siRNA oligos led to the promotion of proliferation of HepG2 cells. FOXJ2 was markedly downregulated in HCC tissues. The expression of FOXJ2 was correlated with tumor size, histological differentiation and metastasis. Low expression levels of FOXJ2 predicted poor prognosis for patients with HCC, suggesting that FOXJ2 may be a candidate prognostic marker of HCC. Depletion of FOXJ2 caused the promotion of HCC cell proliferation, implicating that FOXJ2 may serve an inhibitory role in the regulation of HCC cell proliferation.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Z, Meng G, Wang L, Ma Y and Guan Z: The prognostic role and reduced expression of FOXJ2 in human hepatocellular carcinoma. Mol Med Rep 14: 254-262, 2016.
APA
Zhang, Z., Meng, G., Wang, L., Ma, Y., & Guan, Z. (2016). The prognostic role and reduced expression of FOXJ2 in human hepatocellular carcinoma. Molecular Medicine Reports, 14, 254-262. https://doi.org/10.3892/mmr.2016.5261
MLA
Zhang, Z., Meng, G., Wang, L., Ma, Y., Guan, Z."The prognostic role and reduced expression of FOXJ2 in human hepatocellular carcinoma". Molecular Medicine Reports 14.1 (2016): 254-262.
Chicago
Zhang, Z., Meng, G., Wang, L., Ma, Y., Guan, Z."The prognostic role and reduced expression of FOXJ2 in human hepatocellular carcinoma". Molecular Medicine Reports 14, no. 1 (2016): 254-262. https://doi.org/10.3892/mmr.2016.5261
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Z, Meng G, Wang L, Ma Y and Guan Z: The prognostic role and reduced expression of FOXJ2 in human hepatocellular carcinoma. Mol Med Rep 14: 254-262, 2016.
APA
Zhang, Z., Meng, G., Wang, L., Ma, Y., & Guan, Z. (2016). The prognostic role and reduced expression of FOXJ2 in human hepatocellular carcinoma. Molecular Medicine Reports, 14, 254-262. https://doi.org/10.3892/mmr.2016.5261
MLA
Zhang, Z., Meng, G., Wang, L., Ma, Y., Guan, Z."The prognostic role and reduced expression of FOXJ2 in human hepatocellular carcinoma". Molecular Medicine Reports 14.1 (2016): 254-262.
Chicago
Zhang, Z., Meng, G., Wang, L., Ma, Y., Guan, Z."The prognostic role and reduced expression of FOXJ2 in human hepatocellular carcinoma". Molecular Medicine Reports 14, no. 1 (2016): 254-262. https://doi.org/10.3892/mmr.2016.5261
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