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Article

Syzygium aromaticum water extract attenuates ethanol‑induced gastric injury through antioxidant effects in rats

  • Authors:
    • Seong Eun Jin
    • Mee‑Young Lee
    • In‑Sik Shin
    • Woo‑Young Jeon
    • Hyekyung Ha
  • View Affiliations / Copyright

    Affiliations: K‑herb Research Center, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea, College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Republic of Korea
  • Pages: 361-366
    |
    Published online on: May 13, 2016
       https://doi.org/10.3892/mmr.2016.5269
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Abstract

The aim of the present study was to investigate whether Syzygium aromaticum water extract (SAWE) has a protective effect against ethanol‑induced gastric injury in rats. Acute gastric injury was induced via intragastric administration of absolute ethanol at a dose of 5 ml/kg. SAWE (250 or 500 mg/kg/day) or cimetidine (100 mg/kg/day), which was used as a positive control, were administered to the rats 2 h prior to ethanol administration for 3 days. All rats were sacrificed 24 h following the final ethanol administration. To examine whether SAWE has a gastroprotective effect, assays were performed to assess the contents of malondialdehyde (MDA) and glutathione (GSH), the activities of catalase, glutathione‑S‑transferase and superoxide dismutase, and an immune-linked immunosorbent assay was performed for prostaglandin E2 (PGE2) production in gastric tissues by hematoxylin and eosin and periodic acid-Schiff staining. Histological assessment of the gastric wall was performed. Compared with ethanol treatment alone, treatment with SAWE at a dose of 250 mg/kg/day significantly decreased the gastric MDA content and increased the GSH content, catalase activity, and production of gastric PGE2. Histological assessment showed that SAWE attenuated inflammatory cell infiltration and the loss of epithelial cells. These findings suggested that SAWE protected against ethanol‑induced gastric mucosal injury in the rats. These effects appeared to be associated with antioxidant activity, activation of the production of PGE2, suppression of inflammatory cell infiltration and loss of epithelial cells in the gastric mucosa. Collectively, SAWE may be beneficial in the prevention of gastric disease associated to oxidative stress.
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Copy and paste a formatted citation
Spandidos Publications style
Jin SE, Lee MY, Shin IS, Jeon WY and Ha H: Syzygium aromaticum water extract attenuates ethanol‑induced gastric injury through antioxidant effects in rats. Mol Med Rep 14: 361-366, 2016.
APA
Jin, S.E., Lee, M., Shin, I., Jeon, W., & Ha, H. (2016). Syzygium aromaticum water extract attenuates ethanol‑induced gastric injury through antioxidant effects in rats. Molecular Medicine Reports, 14, 361-366. https://doi.org/10.3892/mmr.2016.5269
MLA
Jin, S. E., Lee, M., Shin, I., Jeon, W., Ha, H."Syzygium aromaticum water extract attenuates ethanol‑induced gastric injury through antioxidant effects in rats". Molecular Medicine Reports 14.1 (2016): 361-366.
Chicago
Jin, S. E., Lee, M., Shin, I., Jeon, W., Ha, H."Syzygium aromaticum water extract attenuates ethanol‑induced gastric injury through antioxidant effects in rats". Molecular Medicine Reports 14, no. 1 (2016): 361-366. https://doi.org/10.3892/mmr.2016.5269
Copy and paste a formatted citation
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Spandidos Publications style
Jin SE, Lee MY, Shin IS, Jeon WY and Ha H: Syzygium aromaticum water extract attenuates ethanol‑induced gastric injury through antioxidant effects in rats. Mol Med Rep 14: 361-366, 2016.
APA
Jin, S.E., Lee, M., Shin, I., Jeon, W., & Ha, H. (2016). Syzygium aromaticum water extract attenuates ethanol‑induced gastric injury through antioxidant effects in rats. Molecular Medicine Reports, 14, 361-366. https://doi.org/10.3892/mmr.2016.5269
MLA
Jin, S. E., Lee, M., Shin, I., Jeon, W., Ha, H."Syzygium aromaticum water extract attenuates ethanol‑induced gastric injury through antioxidant effects in rats". Molecular Medicine Reports 14.1 (2016): 361-366.
Chicago
Jin, S. E., Lee, M., Shin, I., Jeon, W., Ha, H."Syzygium aromaticum water extract attenuates ethanol‑induced gastric injury through antioxidant effects in rats". Molecular Medicine Reports 14, no. 1 (2016): 361-366. https://doi.org/10.3892/mmr.2016.5269
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