Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
July-2016 Volume 14 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2016 Volume 14 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Cloning and functional analysis of human acyl coenzyme A: Cholesterol acyltransferase1 gene P1 promoter

  • Authors:
    • Jing Ge
    • Bei Cheng
    • Benling Qi
    • Wen Peng
    • Hui Wen
    • Lijuan Bai
    • Yun Liu
    • Wei Zhai
  • View Affiliations / Copyright

    Affiliations: Department of Geriatrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China, Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
  • Pages: 831-838
    |
    Published online on: May 18, 2016
       https://doi.org/10.3892/mmr.2016.5295
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Acyl-coenzyme A: cholesterol acyltransferase 1 (ACAT1) catalyzes the conversion of free cholesterol (FC) to cholesterol ester. The human ACAT1 gene P1 promoter has been cloned. However, the activity and specificity of the ACAT1 gene P1 promoter in diverse cell types remains unclear. The P1 promoter fragment was digested with KpnI/XhoI from a P1 promoter cloning vector, and was subcloned into the multiple cloning site of the Firefly luciferase vector pGL3‑Enhancer to obtain the construct P1E‑1. According to the analysis of biological information, the P1E‑1 plasmid was used to generate deletions of the ACAT1 gene P1 promoter with varying 5' ends and an identical 3' end at +65 by polymerase chain reaction (PCR). All the 5'‑deletion constructs of the P1 promoter were identified by PCR, restriction enzyme digestion mapping and DNA sequencing. The transcriptional activity of each construct was detected after transient transfection into THP‑1, HepG2, HEK293 and Hela cells using DEAE‑dextran and Lipofectamine 2000 liposome transfection reagent. Results showed that the transcriptional activity of the ACAT1 gene P1 promoter and deletions of P1 promoter in THP‑1 and HepG2 cells was higher than that in HEK293 and HeLa cells. Moreover, the transcriptional activity of P1E‑9 was higher compared with those of other deletions in THP‑1, HepG2, HEK293 and HeLa cells. These findings indicate that the transcriptional activity of the P1 promoter and the effects of deletions vary with different cell lines. Thus, the P1 promoter may drive ACAT1 gene expression with cell‑type specificity. In addition, the core sequence of ACAT1 gene P1 promoter was suggested to be between -125 and +65 bp.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Tabas I: The stimulation of the cholesterol esterification pathway by atherogenic lipoproteins in macrophages. Curr Opin Lipidol. 6:260–268. 1995. View Article : Google Scholar : PubMed/NCBI

2 

Chang CC, Sakashita N, Ornvold K, Lee O, Chang ET, Dong R, Lin S, Lee CY, Strom SC, Kashyap R, et al: Immunological quantitation and localization of ACAT-1 and ACAT-2 in human liver and small intestine. J Biol Chem. 275:28083–28092. 2000.PubMed/NCBI

3 

Lee O, Chang CC, Lee W and Chang TY: Immunodepletion experiments suggest that acyl-coenzyme A: Cholesterol acyl-transferase-1 (ACAT-1) protein plays a major catalytic role in adult human liver, adrenal gland, macrophages, and kidney, but not in intestines. J Lipid Res. 39:1722–1727. 1998.PubMed/NCBI

4 

Sakashita N, Miyazaki A, Takeya M, Horiuchi S, Chang CC, Chang TY and Takahashi K: Localization of human acyl-coenzyme A: Cholesterol acyltransferase-1 (ACAT-1) in macrophages and in various tissues. Am J Pathol. 156:227–236. 2000. View Article : Google Scholar : PubMed/NCBI

5 

Saraon P, Trudel D, Kron K, Dmitromanolakis A, Trachtenberg J, Bapat B, van der Kwast T, Jarvi KA and Diamandis EP: Evaluation and prognostic significance of ACAT1 as a marker of prostate cancer progression. Prostate. 74:372–380. 2014. View Article : Google Scholar

6 

Murphy SR, Chang CC, Dogbevia G, Bryleva EY, Bowen Z, Hasan MT and Chang TY: Acat1 knockdown gene therapy decreases amyloid-β in a mouse model of Alzheimer's disease. Mol Ther. 21:1497–1506. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Dharuri H, 't Hoen PA, van Klinken JB, Henneman P, Laros JF, Lips MA, El Bouazzaoui F, van Ommen GJ, Janssen I, van Ramshorst B, et al: Downregulation of the acetyl-CoA metabolic network in adipose tissue of obese diabetic individuals and recovery after weight loss. Diabetologia. 57:2384–2392. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Li BL, Li XL, Duan ZJ, Lee O, Lin S, Ma ZM, Chang CC, Yang XY, Park JP, Mohandas TK, et al: Human acyl-coA: Cholesterol acyltransferase-1 (ACAT-1) gene organization and evidence that the 4.3-Kilobase ACAT-1 mRNA is produced from two different chromosomes. J Biol Chem. 274:11060–11071. 1999. View Article : Google Scholar : PubMed/NCBI

9 

Ge J, Zhai W, Cheng B, He P, Qi B, Lu H, Zeng Y and Chen X: Insulin induces human acyl-coenzyme A: Cholesterol acyl-transferase 1 gene expression via MAP kinases and CCAAT/enhancer-binding protein α. J Cell Biochem. 114:2188–2198. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Yang JB, Duan ZJ, Yao W, Lee O, Yang L, Yang XY, Sun X, Chang CC, Chang TY and Li BL: Synergistic transcriptional activation of human Acyl-coenzyme A: Cholesterol acyltransterase-1 gene by interferon-gamma and all-trans-retinoic acid THP-1 cells. J Biol Chem. 276:20989–20998. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Chang TY, Chang CC and Cheng D: Acyl-coenzyme A: Cholesterol acyltransferase. Annu Rev Biochem. 66:613–638. 1997. View Article : Google Scholar

12 

Yang L, Yang JB, Chen J, Yu GY, Zhou P, Lei L, Wang ZZ, Cy Chang C, Yang XY, Chang TY and Li BL: Enhancement of human ACAT1 gene expression to promote the macrophage-derived foam cell formation by dexamethasone. Cell Res. 14:315–323. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Maung K, Miyazaki A, Nomiyama H, Chang CC, Chang TY and Horiuchi S: Induction of acyl-coenzyme A: Cholesterol acyltransferase-1 by 1,25-dihydroxyvitamin D(3) or 9-cis-retinoic acid in undifferentiated THP-1 cells. J Lipid Res. 42:181–187. 2001.PubMed/NCBI

14 

Lei L, Xiong Y, Chen J, Yang JB, Wang Y, Yang XY, Chang CC, Song BL, Chang TY and Li BL: TNF-alpha stimulates the ACAT1 expression in differentiating monocytes to promote the CE-laden cell formation. J Lipid Res. 50:1057–1067. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Hori M, Miyazaki A, Tamagawa H, Satoh M, Furukawa K, Hakamata H, Sasaki Y and Horiuchi S: Up-regulation of acyl-coenzyme A: Cholesterol acyltransferase-1 by transforming growth factor-beta1 during differentiation of human monocytes into macrophages. Biochem Biophys Res Commun. 320:501–505. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Suguro T, Watanabe T, Kanome T, Kodate S, Hirano T, Miyazaki A and Adachi M: Serotonin acts as an up-regulator of acyl-coenzyme A: Cholesterol acyltransferase-1 in human monocyte-macrophages. Atherosclerosis. 186:275–281. 2009. View Article : Google Scholar

17 

Hongo S, Watanabe T, Arita S, Kanome T, Kageyama H, Shioda S and Miyazaki A: Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages. Am J Physiol Endocrinol Metab. 297:E474–E482. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Nagashima M, Watanabe T, Terasaki M, Tomoyasu M, Nohtomi K, Kim-Kaneyama J, Miyazaki A and Hirano T: Native incretins prevent the development of atherosclerotic lesions in apolipoprotein E knockout mice. Diabetologia. 54:2649–2459. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Zhao ZZ, Wang Z, Li GH, Wang R, Tan JM, Cao X, Suo R and Jiang ZS: Hydrogen sulfide inhibits macrophage-derived foam cell formation. Exp Biol Med (Maywood). 236:169–176. 2011. View Article : Google Scholar

20 

Furukawa K, Hori M, Ouchi N, Kihara S, Funahashi T, Matsuzawa Y, Miyazaki A, Nakayama H and Horiuchi S: Adiponectin down-regulates acyl-coenzyme A: Cholesterol acyltransferase-1 in cultured human monocyte-derived macrophages. Biochem Biophys Res Commun. 317:831–836. 2004. View Article : Google Scholar : PubMed/NCBI

21 

Wan JJ, Cheng B, Wang YF, Mei CL, Liu W, Ke L and He P: Ghrelin down-regulates ACAT-1 in THP-1 derived foam cells via growth hormone secretagogue receptor-dependent pathway. Zhonghua Xin Xue Guan Bing Za Zhi. 37:1030–1034. 2009.In Chinese.

22 

Smith JL, Rangaraj K, Simpson R, Maclean DJ, Nathanson LK, Stuart KA, Scott SP, Ramm GA and de Jersey J: Quantitative analysis of the expression of ACAT genes in human tissues by real-time PCR. J Lipid Res. 45:686–696. 2004. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Ge J, Cheng B, Qi B, Peng W, Wen H, Bai L, Liu Y and Zhai W: Cloning and functional analysis of human acyl coenzyme A: Cholesterol acyltransferase1 gene P1 promoter. Mol Med Rep 14: 831-838, 2016.
APA
Ge, J., Cheng, B., Qi, B., Peng, W., Wen, H., Bai, L. ... Zhai, W. (2016). Cloning and functional analysis of human acyl coenzyme A: Cholesterol acyltransferase1 gene P1 promoter. Molecular Medicine Reports, 14, 831-838. https://doi.org/10.3892/mmr.2016.5295
MLA
Ge, J., Cheng, B., Qi, B., Peng, W., Wen, H., Bai, L., Liu, Y., Zhai, W."Cloning and functional analysis of human acyl coenzyme A: Cholesterol acyltransferase1 gene P1 promoter". Molecular Medicine Reports 14.1 (2016): 831-838.
Chicago
Ge, J., Cheng, B., Qi, B., Peng, W., Wen, H., Bai, L., Liu, Y., Zhai, W."Cloning and functional analysis of human acyl coenzyme A: Cholesterol acyltransferase1 gene P1 promoter". Molecular Medicine Reports 14, no. 1 (2016): 831-838. https://doi.org/10.3892/mmr.2016.5295
Copy and paste a formatted citation
x
Spandidos Publications style
Ge J, Cheng B, Qi B, Peng W, Wen H, Bai L, Liu Y and Zhai W: Cloning and functional analysis of human acyl coenzyme A: Cholesterol acyltransferase1 gene P1 promoter. Mol Med Rep 14: 831-838, 2016.
APA
Ge, J., Cheng, B., Qi, B., Peng, W., Wen, H., Bai, L. ... Zhai, W. (2016). Cloning and functional analysis of human acyl coenzyme A: Cholesterol acyltransferase1 gene P1 promoter. Molecular Medicine Reports, 14, 831-838. https://doi.org/10.3892/mmr.2016.5295
MLA
Ge, J., Cheng, B., Qi, B., Peng, W., Wen, H., Bai, L., Liu, Y., Zhai, W."Cloning and functional analysis of human acyl coenzyme A: Cholesterol acyltransferase1 gene P1 promoter". Molecular Medicine Reports 14.1 (2016): 831-838.
Chicago
Ge, J., Cheng, B., Qi, B., Peng, W., Wen, H., Bai, L., Liu, Y., Zhai, W."Cloning and functional analysis of human acyl coenzyme A: Cholesterol acyltransferase1 gene P1 promoter". Molecular Medicine Reports 14, no. 1 (2016): 831-838. https://doi.org/10.3892/mmr.2016.5295
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team