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Article

Astragalus polysaccharide protects human cardiac microvascular endothelial cells from hypoxia/reoxygenation injury: The role of PI3K/AKT, Bax/Bcl-2 and caspase-3

  • Authors:
    • Liandi Xie
    • Yang Wu
    • Zongjing Fan
    • Yang Liu
    • Jixiang Zeng
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, P.R. China, Department of Cardiology, Beijing University of Chinese Medicine, Beijing 100029, P.R. China
  • Pages: 904-910
    |
    Published online on: May 18, 2016
       https://doi.org/10.3892/mmr.2016.5296
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Abstract

In the present study, the mechanisms associated with the Astragalus polysaccharide (APS)-mediated protection of human cardiac microvascular endothelial cells (HCMEC) against hypoxia/reoxygenation (HR) injury were investigated. Pretreatment of HCMECs with APS at various concentrations was performed prior to Na2S2O4-induced HR injury. Subsequently, cell viability and apoptosis were measured by MTT and Hoechst assays, respectively. The viability of HCMECs was reduced by Na2S2O4 and apoptosis was enhanced; however, cell viability was observed to be increased by APS via inhibition of apoptosis. Additionally, intracellular reactive oxygen species (ROS), Ca2+, nitric oxide (NO), malondialdehyde (MDA), superoxide dismutase (SOD), phosphatidylinositol 3-kinase (PI3K)-protein kinase B (AKT), B‑cell lymphoma‑2 (Bcl‑2), Bcl‑2 associated X protein (Bax) and caspase‑3 were measured using detection kits or western blot analysis. In HCMECs with HR injury, the levels of ROS and Ca2+, MDA and Bax expression levels, and the activity of caspase‑3 were elevated. By contrast, the level of NO, the protein expression levels of SOD, Bcl‑2 and PI3K, and the phosphorylation of AKT were decreased. However, compared with the HR group, the effects of HR injury were significantly reduced by APS, with APS providing a protective effect on HCMECs, particularly at higher doses. The current study concluded that APS protects HCMECs from Na2S2O4‑induced HR injury by reducing the levels of ROS, Ca2+, MDA and Bax, inhibiting the activity of caspase‑3, and enhancing the levels of NO, SOD, Bcl‑2, PI3K and phosphorylated AKT. These results may provide an insight into the clinical application of APS and novel therapeutic strategies for HR injury.
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View References

1 

Mangge H, Becker K, Fuchs D and Gostner JM: Antioxidants, inflammation and cardiovascular disease. World J Cardiol. 6:462–477. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Cao G, Cai H, Cai B and Tu S: Effect of 5-hydroxymethylfurfural derived from processed Cornus officinalis on the prevention of high glucose-induced oxidative stress in human umbilical vein endothelial cells and its mechanism. Food Chem. 140:273–279. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Zhang J, Wang Z, Zuo G, Li B, Zhang J, Tian N and Chen S: Low shear stress induces human vascular endothelial cell apoptosis by activating Akt signal and increasing reactive oxygen species. Nan Fang Yi Ke Da Xue Xue Bao. 33:313–317. 2013.PubMed/NCBI

4 

Hu L, Sun Y and Hu J: Catalpol inhibits apoptosis in hydrogen peroxide-induced endothelium by activating the PI3K/Akt signaling pathway and modulating expression of Bcl-2 and Bax. Eur J Pharmacol. 628:155–163. 2010. View Article : Google Scholar

5 

Ginter E, Simko V and Panakova V: Antioxidants in health and disease. Bratisl Lek Listy. 115:603–606. 2014.

6 

Zhang X, Xu X and Wang N: Progress of studies on protective mechanism of Radix Astragali in vascular endothelial cells. Chinese Pharm J. 48:1526–1530. 2013.

7 

Hai-Yan Z, Yong-Hong G, Zhi-Yao W, Bing X, Ai-Ming W, Yan-Wei X, Bei L, Li-Xia L and Li-Xin C: Astragalus polysaccharide suppresses the expression of adhesion molecules through the regulation of the p38 MAPK signaling pathway in human cardiac microvascular endothelial cells after ischemia-reperfusion injury. Evid Based Complement Alternat Med. 2013:2804932013. View Article : Google Scholar : PubMed/NCBI

8 

Xu B, Zhu H, Gao Y, Liu B, Zhu L and Chen L: Influences of Astragalus polysaccharides on genetic transcription of P-selectin and E-selectin in human cardiac microvascular endothelial cells after ischemia-reperfusion injury. J Beijing Univ Tradit Chin Med. 34:177–180. 2011.

9 

Yin Y, Li P, Lu G, Liang J and Zhao F: Protective effects of APS-A3 on blood vessel endothelium function induced by paraoxon. Lishizhen Med Mater Med Res. 22:583–585. 2011.

10 

Li T, Chen L, Li Q, Zhou Y, Tang H and Cheng S: The protective effects of tetramethylpyrazine (TMP) combined with Astragalus polysaccharides (APS) on vascular endothelial cells (VECs). China J Tradit Chin Med Pharm. 26:2672–2675. 2011.

11 

Zhong Y, Cheng C, Huang H, Li A, Liu B and Liu S: Astragalus polysaccharides protects advanced glycation end-products induced endothelial cells apoptosis. Clin Med Eng. 21:18–20. 2014.

12 

Xu H, Wu Q, Xie X and Kong D: Effect of Astragalus polysaccharides on peripheral endothelial progenitor cells via PI3K/Akt/eNOS signal pathway in patients with type 2 diabetes. J Clin Rehabilitative Tissue Eng Res. 15:4272–4276. 2011.

13 

Wang S, Feng Y, Wang L, Wang Y, Xu D and Ruan K: The effect of polysaccharide from Radix Astragali on cells survival against oxygen glucose deprivation and phosphorylation of Akt. Pharm Biotechnol. 18:288–290. 2011.

14 

Zhu H, Chen L and Zhu L: Effect of astragalus polysaccharides on expression of ICAM-1 and VCAM-1 in human cardiac microvascular endothelial cells after hypoxia and reoxygenation. Liaoning J Tradit Chinese Med. 35:293–295. 2008.

15 

Suriyo T, Watcharasit P, Thiantanawat A and Satayavivad J: Arsenite promotes apoptosis and dysfunction in microvascular endothelial cells via an alteration of intracellular calcium homeostasis. Toxicol In Vitro. 26:386–395. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Mukai Y, Shimokawa H, Matoba T, Hiroki J, Kunihiro I, Fujiki T and Takeshita A: Acute vasodilator effects of HMG-CoA reductase inhibitors: Involvement of PI3-kinase/Akt pathway and Kv channels. J Cardiovasc Pharmacol. 42:118–124. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Zhu YP, Shen T, Lin YJ, Chen BD, Ruan Y, Cao Y, Qiao Y, Man Y, Wang S and Li J: Astragalus polysaccharides suppress ICAM-1 and VCAM-1 expression in TNF-α-treated human vascular endothelial cells by blocking NF-ĸB activation. Acta Pharmacol Sin. 34:1036–1042. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Liu M, Qin J, Hao Y, Liu M, Luo J, Luo T and Wei L: Astragalus polysaccharide suppresses skeletal muscle myostatin expression in diabetes: Involvement of ROS-ERK and NF-ĸB pathways. Oxid Med Cell Longev. 2013:7824972013. View Article : Google Scholar

19 

Huang WM, Liang YQ, Tang LJ, Ding Y and Wang XH: Antioxidant and anti-inflammatory effects of Astragalus polysaccharide on EA.hy926 cells. Exp Ther Med. 6:199–203. 2013.PubMed/NCBI

20 

Zuo H, Liao D, Lin L, Zhang R and Li X: Resveratrol attenuates hypoxia-reperfusion injury induced rat myocardium microvascular endothelial cell dysfunction through upregulating PI3K/Akt/SVV pathways. Zhonghua Xin Xue Guan Bing Za Zhi. 42:670–674. 2014.In Chinese. PubMed/NCBI

21 

Su C, Xia T, Ren S, Qing S, Jing D, Lian H, Bin Q, Yuan Z and Xiang Z: Effect of diazoxide preconditioning on cultured rat myocardium microvascular endothelial cells against apoptosis and relation of PI3K/Akt pathway. Balkan Med J. 31:83–87. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Cao Y, Ruan Y, Shen T, Huang X, Li M, Yu W, Zhu Y, Man Y, Wang S and Li J: Astragalus polysaccharide suppresses doxorubicin-induced cardiotoxicity by regulating the PI3k/Akt and p38MAPK pathways. Oxid Med Cell Longev. 2014:6742192014. View Article : Google Scholar : PubMed/NCBI

23 

Ye MN, Chen HF, Zhou RJ and Liao MJ: Effects of Astragalus polysaccharide on proliferation and Akt phosphorylation of the basal-like breast cancer cell line. Zhong Xi Yi Jie He Xue Bao. 9:1339–1346. 2011.In Chinese. View Article : Google Scholar : PubMed/NCBI

24 

Kim BH, Oh I, Kim JH, Jeon JE, Jeon B, Shin J and Kim TY: Anti-inflammatory activity of compounds isolated from Astragalus sinicus L. in cytokine-induced keratinocytes and skin. Exp Mol Med. 46:e872014. View Article : Google Scholar : PubMed/NCBI

25 

Jia Y, Zuo D, Li Z, Liu H, Dai Z, Cai J, Pang L and Wu Y: Astragaloside IV inhibits doxorubicin-induced cardiomyocyte apoptosis mediated by mitochondrial apoptotic pathway via activating the PI3K/Akt pathway. Chem Pharm Bull (Tokyo). 62:45–53. 2014. View Article : Google Scholar

26 

Ge GH, Dou HJ, Yang SS, Ma JW, Cheng WB, Qiao ZY, Hou YM and Fang WY: Glucagon-like peptide-1 protects against cardiac microvascular endothelial cells injured by high glucose. Asian Pac J Trop Med. 8:73–78. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Velotta JB, Kimura N, Chang SH, Chung J, Itoh S, Rothbard J, Yang PC, Steinman L, Robbins RC and Fischbein MP: αB-crystallin improves murine cardiac function and attenuates apoptosis in human endothelial cells exposed to ischemia-reperfusion. Ann Thorac Surg. 91:1907–1913. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Xiao B, Xu Y, He H, Jiang QL, Li SY, Shu HY, Liang EY, Yi ZS, Ye JY, Huang LF, et al: Anti-apoptotic effect of Astragalus polysaccharide on myeloid cells. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 21:1243–1247. 2013.In Chinese. PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xie L, Wu Y, Fan Z, Liu Y and Zeng J: Astragalus polysaccharide protects human cardiac microvascular endothelial cells from hypoxia/reoxygenation injury: The role of PI3K/AKT, Bax/Bcl-2 and caspase-3. Mol Med Rep 14: 904-910, 2016.
APA
Xie, L., Wu, Y., Fan, Z., Liu, Y., & Zeng, J. (2016). Astragalus polysaccharide protects human cardiac microvascular endothelial cells from hypoxia/reoxygenation injury: The role of PI3K/AKT, Bax/Bcl-2 and caspase-3. Molecular Medicine Reports, 14, 904-910. https://doi.org/10.3892/mmr.2016.5296
MLA
Xie, L., Wu, Y., Fan, Z., Liu, Y., Zeng, J."Astragalus polysaccharide protects human cardiac microvascular endothelial cells from hypoxia/reoxygenation injury: The role of PI3K/AKT, Bax/Bcl-2 and caspase-3". Molecular Medicine Reports 14.1 (2016): 904-910.
Chicago
Xie, L., Wu, Y., Fan, Z., Liu, Y., Zeng, J."Astragalus polysaccharide protects human cardiac microvascular endothelial cells from hypoxia/reoxygenation injury: The role of PI3K/AKT, Bax/Bcl-2 and caspase-3". Molecular Medicine Reports 14, no. 1 (2016): 904-910. https://doi.org/10.3892/mmr.2016.5296
Copy and paste a formatted citation
x
Spandidos Publications style
Xie L, Wu Y, Fan Z, Liu Y and Zeng J: Astragalus polysaccharide protects human cardiac microvascular endothelial cells from hypoxia/reoxygenation injury: The role of PI3K/AKT, Bax/Bcl-2 and caspase-3. Mol Med Rep 14: 904-910, 2016.
APA
Xie, L., Wu, Y., Fan, Z., Liu, Y., & Zeng, J. (2016). Astragalus polysaccharide protects human cardiac microvascular endothelial cells from hypoxia/reoxygenation injury: The role of PI3K/AKT, Bax/Bcl-2 and caspase-3. Molecular Medicine Reports, 14, 904-910. https://doi.org/10.3892/mmr.2016.5296
MLA
Xie, L., Wu, Y., Fan, Z., Liu, Y., Zeng, J."Astragalus polysaccharide protects human cardiac microvascular endothelial cells from hypoxia/reoxygenation injury: The role of PI3K/AKT, Bax/Bcl-2 and caspase-3". Molecular Medicine Reports 14.1 (2016): 904-910.
Chicago
Xie, L., Wu, Y., Fan, Z., Liu, Y., Zeng, J."Astragalus polysaccharide protects human cardiac microvascular endothelial cells from hypoxia/reoxygenation injury: The role of PI3K/AKT, Bax/Bcl-2 and caspase-3". Molecular Medicine Reports 14, no. 1 (2016): 904-910. https://doi.org/10.3892/mmr.2016.5296
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