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Article Open Access

Shox2 influences mesenchymal stem cell fate in a co-culture model in vitro

  • Authors:
    • Yuanyuan Feng
    • Pan Yang
    • Shouming Luo
    • Zhihui Zhang
    • Huakang Li
    • Ping Zhu
    • Zhiyuan Song
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Southwest Hospital, Third Military Medical University, Chongqing 400038, P.R. China
    Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 637-642
    |
    Published online on: May 18, 2016
       https://doi.org/10.3892/mmr.2016.5306
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Abstract

Sinoatrial node (SAN) dysfunction is a common cardiovascular problem, and the development of a cell sourced biological pacemaker has been the focus of cardiac electrophysiology research. The aim of biological pacemaker therapy is to produce SAN-like cells, which exhibit spontaneous activity characteristic of the SAN. Short stature homeobox 2 (Shox2) is an early cardiac transcription factor and is crucial in the formation and differentiation of the sinoatrial node (SAN). The present study aimed to improve pacemaker function by overexpression of Shox2 in canine mesenchymal stem cells (cMSCs) to induce a phenotype similar to native pacemaker cells. To achieve this objective, the cMSCs were transfected with lentiviral pLentis‑mShox2‑red fluorescent protein, and then co‑cultured with rat neonatal cardiomyocytes (RNCMs) in vitro for 5-7 days. The feasibility of regulating the differentiation of cMSCs into pacemaker‑like cells by Shox2 overexpression was investigated. Reverse transcription-quantitative polymerase chain reaction and western blotting showed that Shox2‑transfected cMSCs expressed high levels of T box 3, hyperpolarization-activated cyclic nucleotide‑gated cation channel and Connexin 45 genes, which participate in SAN development, and low levels of working myocardium genes, Nkx2.5 and Connexin 43. In addition, Shox2‑transfected cMSCs were able to pace RNCMs with a rate faster than the control cells. In conclusion, these data indicate that overexpression of Shox2 in cMSCs can greatly enhance the pacemaker phenotype in a co-culture model in vitro.
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View References

1 

Miake J, Marbán E and Nuss HB: Biological pacemaker created by gene transfer. Nature. 19:132–133. 2002. View Article : Google Scholar

2 

Tong S, Yao Q, Wan Y, Zhou J, Shu M, Zhong L, Li Y, Zhang Q, Yindai J and Song Z: Development of functional I f channels in mMSCs after transfection with mHCN4: Effects on cell morphology and mechanical activity in vitro. Cardiology. 112:114–121. 2009. View Article : Google Scholar

3 

Jun C, Zhihui Z, Lu W, Yaoming N, Lei W, Yao Q and Zhiyuan S: Canine bone marrow mesenchymal stromal cells with lentiviral mHCN4 gene transfer create cardiac pacemakers. Cytotherapy. 14:529–539. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Lu W, Yaoming N, Boli R, Jun C, Changhai Z, Yang Z and Zhiyuan S: mHCN4 genetically modified canine mesenchymal stem cells provide biological pacemaking function in complete dogs with atrioventricular block. Pacing Clin Electrophysiol. 36:1138–1149. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Cai J, Yi FF, Li YH, Yang XC, Song J, Jiang XJ, Jiang H, Lin GS and Wang W: Adenoviral gene transfer of HCN4 creates a genetic pacemaker in pigs with complete atrioventricular block. Life Sci. 80:1476–1753. 2007. View Article : Google Scholar

6 

Espinoza-Lewis RA, Yu L, He F, Liu H, Tang R, Shi J, Sun X, Martin JF, Wang D, Yang J and Chen Y: Shox2 is essential for the differentiation of cardiac pacemaker cells by repressing Nkx2-5. Dev Biol. 327:376–385. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Espinoza-Lewis RA, Liu H, Sun C, Chen C, Jiao K and Chen Y: Ectopic expression of Nkx2.5 suppresses the formation of the sinoatrial node in mice. Dev Biol. 356:359–369. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Chang MG, Tung L, Sekar RB, Chang CY, Cysyk J, Dong P, Marbán E and Abraham MR: Proarrhythmic potential of mesenchymal stem cell transplantation revealed in an in vitro coculture model. Circulation. 113:1832–1841. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Li Y, Li B, Zhang C, Zhang J, Zeng M and Zheng Z: Effect of NRG-1/ErbB signaling intervention on the differentiation of bone marrow stromal cells into sinus node-like cells. J Cardiovasc Pharmacol. 63:434–440. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Wang T, Xu Z, Jiang W and Ma A: Cell-to-cell contact induces mesenchymal stem cell to differentiate into cardiomyocyte and smooth muscle cell. Int J Cardiol. 109:74–81. 2006. View Article : Google Scholar

11 

Wen L, Zhang C, Nong Y, Yao Q and Song Z: Mild electrical pulse current stimulation upregulates S100A4 and promotes cardiogenesis in MSC and cardiac myocytes coculture monolayer. Cell Biochem Biophys. 65:43–55. 2013. View Article : Google Scholar

12 

Kim MO, Jung H, Kim SC, Park JK and Seo YK: Electromagnetic fields and nanomagnetic particles increase the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells. Int J Mol Med. 35:153–160. 2015.

13 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-timequantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar

14 

Liu H, Chen CH, Ye W, Espinoza-Lewis RA, Hu X, Zhang Y and Chen Y: Phosphorylation of Shox2 is required for its function to control sinoatrial node formation. J Am Heart Assoc. 3. pp. e0007962014, View Article : Google Scholar

15 

Valiunas V, Doronin S, Valiuniene L, Potapova I, Zuckerman J, Walcott B, Robinson RB, Rosen MR, Brink PR and Cohen IS: Human mesenchymal stem cells make cardiac connexins and form functional gap junctions. J Physiol. 555:617–623. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Hoogaars WM, Engel A, Brons JF, Verkerk AO, de Lange FJ, Wong LY, Bakker ML, Clout DE, Wakker V, Barnett P, et al: Tbx3 controls the sinoatrial node gene program and imposes pacemaker function on the atria. Genes Dev. 21:1098–1112. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Wiese C, Grieskamp T, Airik R, Mommersteeg MT, Gardiwal A, de Gier-de Vries C, Schuster-Gossler K, Moorman AF, Kispert A and Christoffels VM: Formation of the sinus node head and differentiation of sinus node myocardium are independently regulated by Tbx18 and Tbx3. Circ Res. 104:388–397. 2009. View Article : Google Scholar

18 

Frank DU, Carter KL, Thomas KR, Burr RM, Bakker ML, Coetzee WA, Tristani-Firouzi M, Bamshad MJ, Christoffels VM and Moon AM: Lethal arrhythmias in Tbx3-deficient mice reveal extreme dosage sensitivity of cardiac conduction system function and homeostasis. Proc Natl Acad Sci USA. 109:E154–E163. 2012. View Article : Google Scholar :

19 

Söhl G and Willecke K: Gap junctions and the connexin protein family. Cardiovasc Res. 62:228–232. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Desplantez T, Dupont E, Severs NJ and Weingart R: Gap junction channels and cardiac impulse propagation. J Membr Biol. 218:13–28. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Feng Y, Yang P, Luo S, Zhang Z, Li H, Zhu P and Song Z: Shox2 influences mesenchymal stem cell fate in a co-culture model in vitro. Mol Med Rep 14: 637-642, 2016.
APA
Feng, Y., Yang, P., Luo, S., Zhang, Z., Li, H., Zhu, P., & Song, Z. (2016). Shox2 influences mesenchymal stem cell fate in a co-culture model in vitro. Molecular Medicine Reports, 14, 637-642. https://doi.org/10.3892/mmr.2016.5306
MLA
Feng, Y., Yang, P., Luo, S., Zhang, Z., Li, H., Zhu, P., Song, Z."Shox2 influences mesenchymal stem cell fate in a co-culture model in vitro". Molecular Medicine Reports 14.1 (2016): 637-642.
Chicago
Feng, Y., Yang, P., Luo, S., Zhang, Z., Li, H., Zhu, P., Song, Z."Shox2 influences mesenchymal stem cell fate in a co-culture model in vitro". Molecular Medicine Reports 14, no. 1 (2016): 637-642. https://doi.org/10.3892/mmr.2016.5306
Copy and paste a formatted citation
x
Spandidos Publications style
Feng Y, Yang P, Luo S, Zhang Z, Li H, Zhu P and Song Z: Shox2 influences mesenchymal stem cell fate in a co-culture model in vitro. Mol Med Rep 14: 637-642, 2016.
APA
Feng, Y., Yang, P., Luo, S., Zhang, Z., Li, H., Zhu, P., & Song, Z. (2016). Shox2 influences mesenchymal stem cell fate in a co-culture model in vitro. Molecular Medicine Reports, 14, 637-642. https://doi.org/10.3892/mmr.2016.5306
MLA
Feng, Y., Yang, P., Luo, S., Zhang, Z., Li, H., Zhu, P., Song, Z."Shox2 influences mesenchymal stem cell fate in a co-culture model in vitro". Molecular Medicine Reports 14.1 (2016): 637-642.
Chicago
Feng, Y., Yang, P., Luo, S., Zhang, Z., Li, H., Zhu, P., Song, Z."Shox2 influences mesenchymal stem cell fate in a co-culture model in vitro". Molecular Medicine Reports 14, no. 1 (2016): 637-642. https://doi.org/10.3892/mmr.2016.5306
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