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Article

SerpinE2 promotes multiple cell proliferation and drug resistance in osteosarcoma

  • Authors:
    • Minzhi Mao
    • Wanchun Wang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China
  • Pages: 881-887
    |
    Published online on: May 19, 2016
       https://doi.org/10.3892/mmr.2016.5316
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Abstract

SerpinE2 is a member of the Serpins family, which could inhibit serine protease and promote tumor progression, particularly in tumor metastasis. However, at present, its role in the progression of osteosarcoma has not been determined. The present study analyzed the expression profiles of SerpinE2 in cancer tissues, including tissues from osteosarcoma of different stages. Higher expression of SerpinE2 was shown in osteosarcoma tissues, particularly in tissue from patients with metastasis and a tumor-node-metastasis stage II‑III. Following chemotherapy, the SerpinE2 expression levels were shown to be higher than those at diagnosis. Cell proliferation and colony formation were increased after transfection with SerpinE2 over‑expression vector. Additionally, drug resistance to bortezomib and doxorubicin treatment following SerpinE2 transfection was analyzed. MG‑63 and SAOS‑2 cells showed less sensitivity following transfection with SerpinE2. The cell cycle‑related genes, cyclin‑dependent kinase (CDK)4 and cyclin D1 were positively correlated with SerpinE2 expression in patient‑derived tissue and in osteosarcoma cells. Finally, the high expression of SerpinE2 contributes to poor survival rates in patients with osteosarcoma. In conclusion, high expression of SerpinE2 in osteosarcoma stimulates cell proliferation, promotes drug‑resistance, and results in poor survival by regulating CDK4 and cyclin D1. Thus, SerpinE2 could be a potential target for treatment of patients with osteosarcoma.
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1 

Siegel R, Ma J, Zou Z and Jemal A: Cancer statistics, 2014. CA Cancer J Clin. 64:9–29. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Janeway KA and Grier HE: Sequelae of osteosarcoma medical therapy: A review of rare acute toxicities and late effects. Lancet Oncol. 11:670–678. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Yang J and Zhang W: New molecular insights into osteosarcoma targeted therapy. Curr Opin Oncol. 25:398–406. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Silverman GA, Bird PI, Carrell RW, Church FC, Coughlin PB, Gettins PG, Irving JA, Lomas DA, Luke CJ, Moyer RW, et al: The serpins are an expanding superfamily of structurally similar but funtionally diverse proteins: Evolution, mechanism of inhibition, novel functions, and a revised nomenclature. J Biol Chem. 276:33293–33296. 2001. View Article : Google Scholar : PubMed/NCBI

5 

Valiente M, Obenauf AC, Jin X, Chen Q, Zhang XH, Lee DJ, Chaft JE, Kris MG, Huse JT, Brogi E and Massagué J: Serpins promote cancer cell survival and vascular co-option in brain metastasis. Cell. 156:1002–1016. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Paik PK, Shen R, Won H, Rekhtman N, Wang L, Sima CS, Arora A, Seshan V, Ladanyi M, Berger MF and Kris MG: Next-Generation sequencing of stage IV squamous cell lung cancers reveals an association of PI3K aberrations and evidence of clonal heterogeneity in patients with brain metastases. Cancer Discov. 5:610–621. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Shiiba M, Nomura H, Shinozuka K, Saito K, Kouzu Y, Kasamatsu A, Sakamoto Y, Murano A, Ono K, Ogawara K, et al: Down-regulated expression of SERPIN genes located on chromosome 18q21 in oral squamous cell carcinomas. Oncol Rep. 24:241–249. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Li Z, Liu Q, Song M, Zheng Y, Nan P, Cao Y, Chen G, Li Y and Zhong Y: Detecting correlation between sequence and expression divergences in a comparative analysis of human serpin genes. Biosystems. 82:226–234. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Erler JT: Cancer: Disabling defences in the brain. Nature. 508:46–47. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Medress Z and Hayden Gephart M: Molecular and genetic predictors of breast-to-brain metastasis: Review and case presentation. Cureus. 7:e2462015.PubMed/NCBI

11 

Nagahara A, Nakayama M, Oka D, Tsuchiya M, Kawashima A, Mukai M, Nakai Y, Takayama H, Nishimura K, Jo Y, et al: SERPINE2 is a possible candidate promotor for lymph node metastasis in testicular cancer. Biochem Biophys Res Commun. 391:1641–1646. 2010. View Article : Google Scholar

12 

Lee RK, Fan CC, Hwu YM, Lu CH, Lin MH, Chen YJ and Li SH: SERPINE2, an inhibitor of plasminogen activators, is highly expressed in the human endometrium during the secretory phase. Reprod Biol Endocrinol. 9:382011. View Article : Google Scholar : PubMed/NCBI

13 

Demeo DL, Mariani TJ, Lange C, Srisuma S, Litonjua AA, Celedon JC, Lake SL, Reilly JJ, Chapman HA, Mecham BH, et al: The SERPINE2 gene is associated with chronic obstructive pulmonary disease. Am J Hum Genet. 78:253–264. 2006. View Article : Google Scholar :

14 

Buchholz M, Biebl A, Neesse A, Wagner M, Iwamura T, Leder G, Adler G and Gress TM: SERPINE2 (protease nexin I) promotes extracellular matrix production and local invasion of pancreatic tumors in vivo. Cancer Res. 63:4945–4951. 2003.PubMed/NCBI

15 

Sarrió D, Rodriguez-Pinilla SM, Hardisson D, Cano A, Moreno-Bueno G and Palacios J: Epithelial-mesenchymal transition in breast cancer relates to the basal-like phenotype. Cancer Res. 68:989–997. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Bergeron S, Lemieux E, Durand V, Cagnol S, Carrier JC, Lussier JG, Boucher MJ and Rivard N: The serine protease inhibitor serpinE2 is a novel target of ERK signaling involved in human colorectal tumorigenesis. Mol Cancer. 9:2712010. View Article : Google Scholar : PubMed/NCBI

17 

Gao S, Krogdahl A, Sørensen JA, Kousted TM, Dabelsteen E and Andreasen PA: Overexpression of protease nexin-1 mRNA and protein in oral squamous cell carcinomas. Oral Oncol. 44:309–313. 2008. View Article : Google Scholar

18 

Neesse A, Wagner M, Ellenrieder V, Bachem M, Gress TM and Buchholz M: Pancreatic stellate cells potentiate proinvasive effects of SERPINE2 expression in pancreatic cancer xenograft tumors. Pancreatology. 7:380–385. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Schajowicz F, Sissons HA and Sobin LH: The World Health Organization's histologic classification of bone tumors. Cancer. 75:1208–1214. 1995. View Article : Google Scholar : PubMed/NCBI

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar

21 

Watanabe R, Iida S, Shimizu Y, Nagata S and Fukunaga R: SEI family of nuclear factors regulates p53-dependent transcriptional activation. Genes Cells. 10:851–860. 2005. View Article : Google Scholar

22 

Kashkar H, Deggerich A, Seeger JM, Yazdanpanah B, Wiegmann K, Haubert D, Pongratz C and Krönke M: NF-kappaB-independent down-regulation of XIAP by bortezomib sensitizes HL B cells against cytotoxic drugs. Blood. 109:3982–3988. 2007. View Article : Google Scholar

23 

Wang K, Wang B, Xing AY, Xu KS, Li GX and Yu ZH: Prognostic significance of SERPINE2 in gastric cancer and its biological function in SGC7901 cells. J Cancer Res Clin Oncol. 141:805–812. 2015. View Article : Google Scholar

24 

Hannemann J, Oosterkamp HM, Bosch CA, Velds A, Wessels LF, Loo C, Rutgers EJ, Rodenhuis S and van de Vijver MJ: Changes in gene expression associated with response to neoadjuvant chemotherapy in breast cancer. J Clin Oncol. 23:3331–3342. 2005. View Article : Google Scholar : PubMed/NCBI

25 

van't Veer LJ, Dai H, Van de Vijver MJ, He YD, Hart AA, Mao M, Peterse HL, van der Kooy K, Marton MJ, Witteveen AT, et al: Gene expression profiling predicts clinical outcome of breast cancer. Nature. 415:530–536. 2002. View Article : Google Scholar

26 

Holleman A, Cheok MH, den Boer ML, Yang W, Veerman AJ, Kazemier KM, Pei D, Cheng C, Pui CH, Relling MV, et al: Gene-expression patterns in drug-resistant acute lymphoblastic leukemia cells and response to treatment. N Engl J Med. 351:533–542. 2004. View Article : Google Scholar : PubMed/NCBI

27 

Vaillant C, Valdivieso P, Nuciforo S, Kool M, Schwarzentruber-Schauerte A, Méreau H, Cabuy E, Lobrinus JA, Pfister S, Zuniga A, et al: Serpine2/PN-1 is required for proliferative expansion of pre-neoplastic lesions and malignant progression to medulloblastoma. PLoS One. 10:e01248702015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Mao M and Wang W: SerpinE2 promotes multiple cell proliferation and drug resistance in osteosarcoma. Mol Med Rep 14: 881-887, 2016.
APA
Mao, M., & Wang, W. (2016). SerpinE2 promotes multiple cell proliferation and drug resistance in osteosarcoma. Molecular Medicine Reports, 14, 881-887. https://doi.org/10.3892/mmr.2016.5316
MLA
Mao, M., Wang, W."SerpinE2 promotes multiple cell proliferation and drug resistance in osteosarcoma". Molecular Medicine Reports 14.1 (2016): 881-887.
Chicago
Mao, M., Wang, W."SerpinE2 promotes multiple cell proliferation and drug resistance in osteosarcoma". Molecular Medicine Reports 14, no. 1 (2016): 881-887. https://doi.org/10.3892/mmr.2016.5316
Copy and paste a formatted citation
x
Spandidos Publications style
Mao M and Wang W: SerpinE2 promotes multiple cell proliferation and drug resistance in osteosarcoma. Mol Med Rep 14: 881-887, 2016.
APA
Mao, M., & Wang, W. (2016). SerpinE2 promotes multiple cell proliferation and drug resistance in osteosarcoma. Molecular Medicine Reports, 14, 881-887. https://doi.org/10.3892/mmr.2016.5316
MLA
Mao, M., Wang, W."SerpinE2 promotes multiple cell proliferation and drug resistance in osteosarcoma". Molecular Medicine Reports 14.1 (2016): 881-887.
Chicago
Mao, M., Wang, W."SerpinE2 promotes multiple cell proliferation and drug resistance in osteosarcoma". Molecular Medicine Reports 14, no. 1 (2016): 881-887. https://doi.org/10.3892/mmr.2016.5316
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