Yifuning postpones ovarian aging through antioxidant mechanisms and suppression of the Rb/p53 signal transduction pathway

Liang,Lei; Zhang,Xu‑Hui; Ji,Bo; Yao,Hui; Ling,Xiao‑Mei; Guo,Zhi‑Jian; Deng,Hong‑Zhu; Wu,Xin‑Rong

doi:10.3892/mmr.2016.5322

Yifuning postpones ovarian aging through antioxidant mechanisms and suppression of the Rb/p53 signal transduction pathway

Authors:
- Lei Liang
- Xu‑Hui Zhang
- Bo Ji
- Hui Yao
- Xiao‑Mei Ling
- Zhi‑Jian Guo
- Hong‑Zhu Deng
- Xin‑Rong Wu
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Affiliations: Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, Guangdong 510010, P.R. China, Department of Oncology, Guangdong No. 2 Provincial People's Hospital, Guangzhou, Guangdong 510317, P.R. China, Department of Pharmacy, The Second People's Hospital of Foshan, Foshan, Guangdong 528000, P.R. China, Department of Urology, Southern Hospital of Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
Published online on: May 23, 2016 https://doi.org/10.3892/mmr.2016.5322
Pages: 888-896

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Abstract

Yifuning is a traditional Chinese medicine recipe that has been used for many years in China for its effects on treating climacteric syndrome in women. The present study aimed to demonstrate the effects and underlying molecular mechanism of Yifuning on the ovaries of aging rats. Selected aging rats were administered different doses of Yifuning (1.0 or 2.0 g/kg by lavage), and after 6 weeks the rats were sacrificed. The activit of indicators of oxidative stress in the serum were measured. The expression levels of 8-oxo-2'-deoxyguanosine (8-OHDG) and p53 in the ovaries were examined using immunohistochemistry. The expression levels of the corresponding genes and proteins were detected by reverse transcription‑quantitative polymerase chain reaction and western blotting analyses, respectively. The results indicated that Yifuning significantly prevented ovarian failure, as indicated by improvements in estrous cycling, reproductive organ weights and sex hormone serum levels. Yifuning significantly increased the levels of superoxide dismutase, glutathione peroxidase, catalase and reduced malondialdehyde and hydrogen peroxide levels. Yifuning reduced DNA damage in the ovaries by reducing the expression of 8‑OHDG and p53. Treatment with Yifuning significantly reduced the age‑induced p19, p53, p21 and Rb activity in the ovaries. The present study demonstrates that Yifuning prevents ovarian failure and the mechanism involved is partly associated with antioxidants and suppression of the Rb/p53 signal transduction pathway.

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