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Iptakalim influences the proliferation and apoptosis of human pulmonary artery smooth muscle cells

  • Authors:
    • Qinglin Li
    • Xiaopei Yan
    • Hui Kong
    • Weiping Xie
    • Hong Wang
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, The First Affiliated Clinical Medical College of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 715-720
    |
    Published online on: May 24, 2016
       https://doi.org/10.3892/mmr.2016.5333
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Abstract

The aim of the present study was to determine the effect of an ATP-sensitive K+ (KATP) channel opener iptakalim (IPT) on the proliferation and apoptosis of human pulmonary artery smooth muscle cells (HPASMCs), and examine the potential value of IPT to hypoxic pulmonary hyper­tension (HPH) at a cellular level. HPASMCs were divided into the control, ET-1, ET-1+IPT and ET-1+IPT+glibenclamide (GLI) groups. GLI was administered 30 min prior to ET-1 and IPT. The 4 groups were incubated with corresponding reagents for 24 h. Cell viability was evaluated using a CCK-8 assay, cell proliferation by 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay, and cell apoptosis via the expression of apoptosis-related proteins, i.e., Bcl-2-associated X protein (Bax) and B-cell lymphoma 2 (Bcl-2) using western blotting. We incubated HPASMCs with varying concentrations of ET-1 for 24, 48 and 72 h, and found that cell survival rate was increased in a dose-dependent manner (P<0.05) rather than in a time-dependent manner (P>0.05). After co-incubation of HPASMCs with varying concentrations of IPT and ET-1 for 24 h, the cell survival rate was decreased in a dose-dependent manner. The cell survival rate in the IPT+ET-1 group was significantly lower than that in the ET-1 group (P<0.05). The cell viability (P<0.05) and proliferation (P<0.05) in the ET-1 group were higher than those in the control group, and the expression of Bax/Bcl-2 was lower than the control group (P<0.05). The cell viability (P<0.05) and proliferation (P<0.05) in the ET-1+IPT group were lower than those in the ET-1 group, and the expression of Bax/Bcl-2 was higher than that in the ET-1 group (P<0.05). The cell viability (P<0.05) and proliferation (P<0.05) in the ET-1+IPT+GLI group were higher than those in the ET-1+IPT group, and the expression of Bax/Bcl-2 was lower than that in the ET-1+IPT group (P<0.05). In conclusion, IPT inhibited ET-1‑induced HPASMC proliferation and promoted cell apoptosis. Thus, it may play an important role in the treatment of HPH.
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Copy and paste a formatted citation
Spandidos Publications style
Li Q, Yan X, Kong H, Xie W and Wang H: Iptakalim influences the proliferation and apoptosis of human pulmonary artery smooth muscle cells. Mol Med Rep 14: 715-720, 2016.
APA
Li, Q., Yan, X., Kong, H., Xie, W., & Wang, H. (2016). Iptakalim influences the proliferation and apoptosis of human pulmonary artery smooth muscle cells. Molecular Medicine Reports, 14, 715-720. https://doi.org/10.3892/mmr.2016.5333
MLA
Li, Q., Yan, X., Kong, H., Xie, W., Wang, H."Iptakalim influences the proliferation and apoptosis of human pulmonary artery smooth muscle cells". Molecular Medicine Reports 14.1 (2016): 715-720.
Chicago
Li, Q., Yan, X., Kong, H., Xie, W., Wang, H."Iptakalim influences the proliferation and apoptosis of human pulmonary artery smooth muscle cells". Molecular Medicine Reports 14, no. 1 (2016): 715-720. https://doi.org/10.3892/mmr.2016.5333
Copy and paste a formatted citation
x
Spandidos Publications style
Li Q, Yan X, Kong H, Xie W and Wang H: Iptakalim influences the proliferation and apoptosis of human pulmonary artery smooth muscle cells. Mol Med Rep 14: 715-720, 2016.
APA
Li, Q., Yan, X., Kong, H., Xie, W., & Wang, H. (2016). Iptakalim influences the proliferation and apoptosis of human pulmonary artery smooth muscle cells. Molecular Medicine Reports, 14, 715-720. https://doi.org/10.3892/mmr.2016.5333
MLA
Li, Q., Yan, X., Kong, H., Xie, W., Wang, H."Iptakalim influences the proliferation and apoptosis of human pulmonary artery smooth muscle cells". Molecular Medicine Reports 14.1 (2016): 715-720.
Chicago
Li, Q., Yan, X., Kong, H., Xie, W., Wang, H."Iptakalim influences the proliferation and apoptosis of human pulmonary artery smooth muscle cells". Molecular Medicine Reports 14, no. 1 (2016): 715-720. https://doi.org/10.3892/mmr.2016.5333
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